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CYTENA x.sight, Руководство пользователя программного обеспечения

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x.sight - software manual 

6 |

 

P a g e

 

2 

Technical information 

2.1  General information: 

 

Platform 

Microsoft Windows 

Framework 

.NET 4.6.x or higher 

DLLs 

64-bit 

Supported OS 

Windows 7 (x64) 

Windows 10 (x64) 

Automation interface 

API and DLL available 

Installation  

msi self-installer 

Space requirements for installation 

400 MB hard drive capacity on C:\ 

Updates  

Download (as installer) 

Internet connection 

Not required 

 

2.2  Embedded PC information: 

 

OS 

Win7 (x64) Professional  (until 2019) 

Win10 (x64) Professional  (from 2020) 

CPU 

Intel Core i7 6700 @ 3.4 GHz 

RAM 

8 GB DDR-4 

Hard drive 

240 GB SSD, SATA M2 

Partition C:\  (OS) 

100 GB 

Partition D:\  (data) 

130 GB 

External interfaces  

1x  DVI 

1x  Displayport (DP++) 

2x  GigE LAN 

4x  USB-3.0 

 

2.3  Data properties: 

 

Program log data format 

XML 

Image data format 

JPEG 

Image resolution 

1.3 Mpixels  (1280x1024) 

Image color format 

8-bit greyscale 

32-bit RGB   (f.sight) 

Images per single-cell 

5 

15   (f.sight) 

Data volume per single-cell 

1.2 MB 

5.0 MB   (f.sight) 

Data volume per 96-well plate 

115 MB 

480 MB   (f.sight) 

Содержание x.sight

Страница 1: ...x sight software manual cytena GmbH Supporting software versions 1 0 7 or higher...

Страница 2: ...mechanical or chemical processes It is possible that the present manual still contains typographical or printing errors The information in this manual however is regularly reviewed and corrections are...

Страница 3: ...ain screen DISPENSE 15 3 6 Cell Camera Region of Interest ROI 18 3 7 Results 21 3 8 Live log window 22 4 f sight special 24 4 1 Cell camera modes 24 4 2 Camera alignment mode 25 4 3 Collect sample mod...

Страница 4: ...x sight software manual 4 P a g e 6 2 Domain domain users 35 6 3 Connection to local network 35 6 4 Connection of additional drives 35...

Страница 5: ...tention These are color coded like shown below Example this is an example text Hint this is a note text Disclaimer of warranty The x sight device is designed for research and may not be used for diagn...

Страница 6: ...ired 2 2 Embedded PC information OS Win7 x64 Professional until 2019 Win10 x64 Professional from 2020 CPU Intel Core i7 6700 3 4 GHz RAM 8 GB DDR 4 Hard drive 240 GB SSD SATA M2 Partition C OS 100 GB...

Страница 7: ...al peripheral standards The following conditions shall be met Min monitor resolution 1920x1080 HD Compatible monitor interfaces DVI HDMI Displayport DP Compatible keyboard interface USB 2 Compatible m...

Страница 8: ...is depends on the configuration and device type you have For any question please contact technical support 3 1 General handling The software contains four main tabs LOAD SETTINGS DROPLET QC and DISPEN...

Страница 9: ...permanently deleted and has to be re added if required The Load Program button in the top right corner can be used to directly load an existing program This will not add a tile to the start screen but...

Страница 10: ...age path Drive D is recommended Make sure that the location has sufficient free space for saving data The section Dispensing represents the possibility to enable an automatic idle dispensing mode call...

Страница 11: ...ically taken over and is set permanently until changed even when software is restarted Hint The cell detection is sensitive against this value Too low values will generate noise and ghost objects that...

Страница 12: ...droplet positioning For example 384 well plates with conical wells for PCR or sequencing applications It can also be useful when targeting special plates or special applications where the position of...

Страница 13: ...osition you find three sliders that help to center the droplet in the cameras field of view and bring it in focus Vertical Delay time delay between drop generation and photo acquisition Horizontal hor...

Страница 14: ...ld disappear in the camera window This is the proof that the shutter system is working for the given piezo parameters and the used cartridge and sample Show the droplet not disappear the system is not...

Страница 15: ...te ID field you can enter your plate barcode or plate name Here you can use the included barcode scanner to read the barcode from your plate into the field The SAVE AS button let you save the current...

Страница 16: ...ontains one fluorescent cell1 Print NTC prints a void single droplet no cell inside Print every droplet prints a single droplet independent from its content Hint Besides printing single cells the soft...

Страница 17: ...or code red Sliders for size and roundness visible below plate Different configurations on different wells appear as different shades of color code Dispense NTC Color code grey Sliders invisible Diffe...

Страница 18: ...e according to its state There are basically three different states not processed The well is colored with respect to the chosen Action e g green for Print fluorescent cell in process When the system...

Страница 19: ...chematic below Only when a single cell is detected inside region A inner blue circle and no other object is inside region B between the two blue circles the single cell is printed to the substrate The...

Страница 20: ...e the zoom function to enlarge the view To do so press the Roi view button below the Cell Camera window The camera will now show the area around the ROI in higher magnification Click in the window to...

Страница 21: ...oints from the graph A counter Data Points right indicates the number of collected data points Example Typically cells e g CHO HEK etc range from 10 to 25 m in size and 0 5 to 0 9 in roundness Double...

Страница 22: ...dow The following parameters are measured for each object Is printed shows if an object was printed into the substrate Diameter m shows the average diameter of the object Area m shows the area of the...

Страница 23: ...of logged data are displayed in the window As soon as this number is exceeded the oldest entry is deleted and replaced by the latest The bottom line gives you more information about the current log bu...

Страница 24: ...d contour The following image shows the difference when Overlay is off left picture and on right picture The blue circles represent the ROI the green contour represents the cell Hint Using the overlay...

Страница 25: ...ence match with each other The alignment mode is automatically started with a dialog when either Collect Sample or PLAY buttons are pressed It is recommended to run this alignment once for each new ca...

Страница 26: ...ontour and the white object Make sure that contour and object superpose each other like shown in A below Cases where both do not superpose B C where only the blue contour is present but no object D or...

Страница 27: ...ain press Retry You can also exit the dialog and return to the main window by pressing Cancel 4 3 Collect sample modes The sample mode is extended to display two types of data dot plots One is the sta...

Страница 28: ...ike to use and click on the respective tile e g on 96 WELL PROGRAMS When choosing a favorite use the small tiles 5 3 Set up your experiment In the SETTINGS tab set the boundary conditions for your exp...

Страница 29: ...plate You can either select individual wells by left click and combine multiple wells by holding CTRL and left click select all wells by clicking in the top left corner of the interactive plate select...

Страница 30: ...only the specific options being available for this action The following example show how individual wells can be independently configured The displayed color code refers to the Action defined for thi...

Страница 31: ...Example 4 limiting dilution approach for rare samples Every droplet is delivered 5 5 Loading cartridge and well plate Before you can actually start your experiment you have to load a cartridge with yo...

Страница 32: ...ndow as three circles blue green blue 5 7 Performing Droplet QC Before starting an experiment with a new loaded cartridge you must perform droplet quality control DQC To enter DQC mode press the DROPL...

Страница 33: ...ngle stable droplet in the image window check the vacuum shutter To do so activate the Turn on check box under Vacuum Settings When being turned on the droplet should disappear in the image window If...

Страница 34: ...Refer to the OPTIONS menu to see where this is Every experiment is stored in a separate folder which is named with date and time All specific information from you such as Name Description etc is stor...

Страница 35: ...ers have to be set such that the cytena application can be executed by the logged in user and it can save data images log files at the specified hard drive paths Data path can be chosen by the operato...

Страница 36: ...ands and Trademarks The cytena logo the name cytena the product series name x sight and the product names c sight f sight and b sight are registered trademarks of cytena GmbH in Europe and or other re...

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