x.sight - software manual
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P a g e
•
Bright field:
shows the bright field channel only (like in c.sight)
•
Fluorescence:
shows the fluorescence channel only
•
BF + FL:
shows a combined image of both channels
4.2 Camera alignment mode
The two independent cameras have to be aligned by software before the system is used
to sort cells. This ensures that both channels (bright field and fluorescence) match with
each other. The alignment mode is automatically started with a dialog when either [
Collect
Sample]
or [
PLAY
] buttons are pressed.
It is recommended to run this alignment once for each new cartridge. Although the dialog
is always displayed, you can skip this step by pressing [
No
] every time. The algorithm will
then use the settings from the run before. Chose [
Yes
] to start the alignment process.
The algorithm will run collect samples in the background and perform the matching on the
acquired cell images from both channels. You will get the results displayed in the dialog:
