CURIOSIS FACSCOPE B Скачать руководство пользователя страница 38 | Manualshive

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B. View Result image

FACSCOPE B provide the result image after counting. FACSCOPE B take multiple image per a channel
depending on count mode and analyze those images. “Result image” menu shows the analyzed
images that labels appended to. Live cells are circled in green and dead cells are circled in red on the
screen.

STEP 1.

Press a

channel

which you want to check, and switch to the

Image

icon.

STEP 2.

Adjust Live / Dead cell definition.

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Содержание FACSCOPE B

Страница 1: ...Automatic Cell Counter Instruction manual...

Страница 2: ...IS Inc E mail info curiosis co kr Website http www curiosis com CURIOSIS Inc 10 Teheran ro 38 gil Gangnam gu Seoul Republic of Korea Tel 82 2 508 5236 Fax 82 2 508 5246 Copyright 2019 by CURIOSIS Inc...

Страница 3: ...ide for 200 tests Optional 50 ea per a box Keypad Optional 1 Barcode scanner Optional 1 Thermal printer Optional 1 When receiving the package Check that all items listed above are included in your pac...

Страница 4: ...sing personal injury or device damage Place the device around 10 cm away from the surroundings for proper air cooling Do not disassemble the device and contact an authorized service center if required...

Страница 5: ...ion range 1 x 104 1 x 107 cells mL Detectable cell diameter 5 60 m Measuring speed Quick mode 20s per test Normal mode 30s per test Precise mode 100s per test Counting area Quick mode 0 15 L Normal mo...

Страница 6: ...ew Slide holder door Slide holder is ejected from inserted into the device Touch LCD display Preview automatic cell counting processes and the results are displayed 3 control buttons Slide holder door...

Страница 7: ...these ports Ethernet port LAN cable is connected to this port for PC interface Power switch Main device power ON OFF control Power cable socket Power cable is connected to this socket USB Port Keypad...

Страница 8: ...tests per a box Cat No CRFCB CSD50 Getting started Pre requirements 12 Basic installation 13 Power up and initial display 14 General operation Sample preparation 15 Basic operation 16 Preview prior t...

Страница 9: ...mory 41 E Exporting Data all history into USB memory 43 F Showing ID 46 On the Setting screen 47 A Checking Firmware information and Update Firmware 48 B Bead Quality Control 50 C Setting Time 52 Main...

Страница 10: ...o distinguish between live and dead cells The stained sample is pipetted on the disposable plastic slide 4 tests per 1 slide and the slide is loaded into the FACSCOPETM B After loading the slide optic...

Страница 11: ...ng with automatic cell counter FACSCOPETM B as well as manual counting method Measuring range of cell concentration is 1 x 104 1 x 107 per mL when using with FACSCOPE B For cell counting prepare cell...

Страница 12: ...d to operate the device at low temperature condition below 10 C In those conditions warm up the device for over 10 minutes Relative humidity between 0 95 Place at corrosive gases free or other substan...

Страница 13: ...ce on a flat and dry surface 2 Plug accompanying power cable into the power cable socket 3 Connect optional peripherals keypad barcode scanner or thermal printer to the USB port if necessary 4 Switch...

Страница 14: ...booting is completed initializing process starts and internal motorized stages start moving 2 Initializing progress is displayed while processing 3 When initializing is finished the slide holder is e...

Страница 15: ...Before sampling the cell suspension gently resuspend the cells at least 6 times pay attention to avoid bubbles and check if there are any cell clumps or agglomerates The sampling should be in middle...

Страница 16: ...the sample onto the slide holder NOTE Make sure the arrow on the slide points toward the instrument STEP 2 Press Start button to start counting procedures The slide holder is inserted automatically a...

Страница 17: ...owing image For completion of each sample the count result unit x104 mL are displayed STEP 4 Once counting is complete the slide holder is ejected automatically Remove FACSCOPE Slide from the slide ho...

Страница 18: ...creen where you can see the cells when tap the screen twice some icons disappear To get icons again tap the screen twice STEP 1 Load a slide and press Preview button STEP 2 Select a channel to preview...

Страница 19: ...TEP 4 See the cell image of the selected channel STEP 5 If press Mark the detection mark is displayed according to the selected preset Live Dead definition can be modified at this stage STEP 6 Countin...

Страница 20: ...while counting Press STOP button STEP 2 Confirmation message box is displayed as shown the following image Press Continue button STEP 3 Once making a stop is confirmed all remaining process are stoppe...

Страница 21: ...mal Precise Total counting area The number of snapshots is different from each count mode Quick mode 0 15 L 1 Frame Normal mode 0 9 L 6 Frames Precise mode 3 6 L 24 Frames Preset User changeable param...

Страница 22: ...d numerous results autosaved after counting The auto saved results review screen are accessible only to the user group that was active at the time the results were published Note Review and User prese...

Страница 23: ...table Count mode the number of frames captured per a channel Analyzed volume Counting time per a test In the case Quick mode 1 0 15 L 20s When you want to get a result quickly and make a rough estimat...

Страница 24: ...E The setting is applied to all enabled channels C Creating Preset Users can manage User Preset items 5 User presets per User group 3 fixed presets cannot be removed or edited STEP 1 To create your ow...

Страница 25: ...25 STEP 2 Press Plus button STEP 3 Select one of 3 fixed presets Universal Small Angular and press blank beside Index STEP 4 Type the names of Index and Preset ID primary T PT primary T...

Страница 26: ...26 STEP 5 Adjust 3 kinds of parameters Gating size Aggregation level Live Dead definition STEP 6 Ready to count with a customized preset PT...

Страница 27: ...27 D Editing Preset STEP 1 To edit your own preset press Preset button STEP 2 Press the preset button which you created by yourself STEP 3 Adjust the parameters of your own preset PT PT...

Страница 28: ...28 STEP 4 Press Save button to keep the changed parameters STEP 5 To delete your own preset press Delete button PT primaryT PT...

Страница 29: ...nel Four channels in the FACSCOPE Slide can be individually enabled or disabled STEP 1 Press the Channels to be disabled enabled Disabled Gray box Enabled White box STEP 2 Press the Start button to co...

Страница 30: ...les loaded onto the slide can be entered by Screen typing Keypad or barcode scanner The maximum of channel ID length is 20 characters in English Arabic numerals and some special characters Basically S...

Страница 31: ...31 STEP 3 Type the names of each Channel ID STEP 4 Press Back button STEP 5 Ready to count JurkatT JurkatT NIH Hela U937 Channel ID...

Страница 32: ...ce STEP 1 Press All button STEP 2 Enter the name of cell type and press OK button STEP 3 Check if 4 channel IDs are written then press Back button Channel ID JurkatT All JurkatT_1 JurkatT_2 JurkatT_3...

Страница 33: ...1 Enter a channel ID and press Enter key 2 Key cursor moves to the next channel ID form It is available to move to the other channel using direction key Barcode Scanner 1 Scan a barcode containing ch...

Страница 34: ...or barcode scanner refer to the above table The maximum of channel ID length is 20 characters in English Arabic numerals and some special characters STEP 3 Check if 4 channel IDs are written then pre...

Страница 35: ...l and combined histogram of all channels FACSCOPE B can detect 5 60 m objects in diameter However the gating system is set to count from 8 m by default because the common cell line size is higher than...

Страница 36: ...value is saved in the new preset Save in the current preset The changed value is saved in current preset This is not available in a fixed preset Apply all The changed value is applied to all channels...

Страница 37: ...37 STEP 3 Move both columns and adjust the cell size STEP 4 Check the results 19 12 26...

Страница 38: ...el depending on count mode and analyze those images Result image menu shows the analyzed images that labels appended to Live cells are circled in green and dead cells are circled in red on the screen...

Страница 39: ...39 STEP 3 Press Data icon STEP 4 Check the number of Live cell and Viability 60...

Страница 40: ...se a thermal printer to printout the counting result Thermal printer is optional Contact your local distributor if required Step 1 Connect thermal printer to the USB port at the back side of the devic...

Страница 41: ...T File system formatted USB memory USB memory icon is present but error message Unsupported USB memory is displayed when trying to export data or report Please use USB memory included in FACSCOPE B pa...

Страница 42: ...s dialog box disappears and notification message Export success is displayed on status bar remove the USB memory from USB port NOTE If the USB memory is removed before the message disappear the result...

Страница 43: ...rmat which can be opened by Microsoft Excel USB memory formatted to ex FAT file system is unsupported In case of connecting ex FAT File system formatted USB memory USB memory icon is present but error...

Страница 44: ...ted user group Connect USB Memory to the USB port at the back side of the device Check if icon is present on the status bar Press Export CSV Step 4 A progress dialog box appears to inform that exporti...

Страница 45: ...s dialog box disappears and notification message Exported all data is displayed on status bar remove the USB memory from USB port NOTE If the USB memory is removed before the message disappear the res...

Страница 46: ...46 F Showing ID Step 1 To see each of Channel IDs press Channel ID To go back press Back...

Страница 47: ...47 On the Setting screen FN...

Страница 48: ...p 1 Press F W info Update and connect USB memory Step 2 Choose the category to update Main or Display If USB memory is disconnected or it doesn t have updated program the message will notice it Step 3...

Страница 49: ...49 Step 4 Updating Step 5 The device will start again automatically with updated version Check if the version is changed Step 6 After about 1 minute switch off and on for stable operation...

Страница 50: ...age Please wait appear on initialization screen after update F W please wait 2 3 minutes do not turn the device off immediately B Bead Quality Control Step 1 Press Bead QC button Step 2 Load a standar...

Страница 51: ...nting Step 4 Check the result amount Step 5 Check the Histogram and Bead image 200 12 Check the image STOP 12 34 12 Home 200 12 Check the image 320 15 Check the image 400 17 Check the image 350 19 Che...

Страница 52: ...52 Step 6 Return to Home screen C Setting Time STEP 1 Press Time button STEP 2 Adjust date and time...

Страница 53: ...CSCOPETM B does not require regular maintenance or replacement of consumable parts Clean the exposed surface of the device using a soft cloth Isopropyl alcohol or deionizes water can be used together...

Страница 54: ...w cell concentration 5 x 104 Try again using Precise mode Cell size is smaller than 10 m or around 10 m Change the gating size parameter in histogram The ratio of trypan blue in counting sample is les...

Страница 55: ...55 Appendix B Examples of error and inaccurate result 1 Too Low error 2 Too High error...

Страница 56: ...or The severely aggregated cell The sample loaded into the slide is dry out 4 the usage for contaminated stain solution The Cells with the contaminated trypan blue Comparative data The Cells with the...

Страница 57: ...nnel No Channel number Channel ID Channel ID Date Measurement date Time Measurement time Total cell x10 4 mL Total cell Count result x 1X104 cells mL Converted count result Live cell x10 4 mL Live cel...

Страница 58: ...58 Appendix D Example and explanation of PDF report...

Страница 59: ...ged without prior consent or notification Copyright 2019 by CURIOSIS Inc All rights reserved Published in Korea Revision History Date Revision Description 2019 03 05 V 0 0 Initially issue 2019 03 20 V...

Страница 60: ...il info curiosis co kr Website http www curiosis com CURIOSIS Inc 10 Teheran ro 38 gil Gangnam gu Seoul Republic of Korea Tel 82 2 508 5236 Fax 82 2 508 5246 Copyright 2019 by CURIOSIS Inc All rights...

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