After the
installation
click Finish to
close the setup
program
Turn on the Mini8 Plus System
Turn on the Mini8 Plus instrument, then double-click the Mini8 Plus icon
to start the software.
*When the Power indicator lights on the front panel stop flashing and
remain solid, the instrument is ready.
Mini8 Plus System Workflow
1. Prepare the sample strip, load it into the Mini8 Plus, and close the lid.
2. Double-click the Mini8 Plus icon on desktop to open the
software.
3. Define and name the experiment, save the experiment.
Tip: to use a pre-defined thermal profile and plate layout for your
experiment, click and select one of the template experiments
saved in your computer.
4. Review the thermal profile and adapt it if needed.
5. Set up the plate layout by defining assays, samples, and standards and
assigning them to wells.
6. Start the run. The Monitor Run tab opens.(Do not open the lid while a
run is in progress.This will corrupt the data.)
7. When the run is completed, open the Mini8 Plus lid. Remove the strip
from the block. Dispose of any hazardous materials into appropricate
containers for biohazard, caustic materials, according to your local safety
regulations.
Load reaction tubes
1. Thaw all necessary reagents (templates, primers, probes, and master
mix).
2. Turn on the computer, then the Mini8 Plus, and wait until the Mini8 Plus
"Power" light is flashing (Standby mode)
3. Confirm that the block and optical path are clear of visible
contaminants and there is no physical damage to the system.
4. Pipette samples and qPCR reagents into the strip according to your
protocol. (Warning: Wear protective gloves and eyewear when operating
with any material that might be considered caustic or hazardous.)
5. Open the Mini8 Plus lid and place the strip on the dock.
6. Close the Mini8 Plus lid.
7. Proceed to define a new experiment.
Workflow
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