5. Set up fluorophore CH1(None/SYBR/FAM)
6. Set up fluorophore CH2(None/Texas Red/ROX)*
7. Click to save plate layout
8. Click to choose and use the saved plate
Click to enter the running interface, and click to
start the running.
Click to pause the running.
Click to stop the running.
Monitor Run
Cycle Numbers(You can increase and
reduce the cycle numbers during the cycling)
Data Collection Point
Melt Curve
Drag Bar Up or Down to Adjust
Temperature and Duration/
Click Temperature Plateau to Adjust
Temperature and Duration
Remaining Time
Warning
Do not open the lid while a run is in progress. It may allow extraneous
light enter the system so the data will be corrupted.
Note
If you do not set up the fluorophore channel when set the thermal profile,
there will be not any curve showing neither during nor after the process
of amplification, for the fluorescence will not be read.
When the running is over, click to save data(*.edt).
The Real time PCR curve will be showed during the amplification.
Channel switch
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