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Overview

Introduction

Setup

Unpack the Mini8 Plus System

Place Mini8 Plus on the Bench

Connect Mini8 Plus 

Install the Mini8 Plus Software

Turn on the Mini8 Plus System

Workflow

Mini8 Plus System Workflow

Load the reaction tubes

Define a New Experiment

Set Up the Thermal Profile

Define the Plate Layout

Monitor Run

Data Analysis

System Information

Lights

Specifications and Environmental Requirements

Electromagnetic Compatibility

Cleaning and Maintenance

Concepts

Chapter 1 

Chapter 2 

Chapter 3

Chapter 4 

Chapter 5 

Contents

1

Содержание Mini8 Plus

Страница 1: ...angdixinxilu 12 Haidian District Beijing China 100085 www coyotebio com Mini8 Plus Real Time PCR System Coyote highly specialized in research development offers integrate molecular diagnostic solutions by providing devices from sample pre to gene amplification detection Version EN 20160413 ...

Страница 2: ...ystem Workflow Mini8 Plus System Workflow Load the reaction tubes Define a New Experiment Set Up the Thermal Profile Define the Plate Layout Monitor Run Data Analysis System Information Lights Specifications and Environmental Requirements Electromagnetic Compatibility Cleaning and Maintenance Concepts Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Contents 1 ...

Страница 3: ...Registered Address 509 510 5th floor Chuangyezhonglu 36 Haidian Beijing ZIP 100085 TEL 010 64844237 FAX 010 64844237 8000 SALES sales coyotebio com Overview 2 Unpack the Mini8 Plus System 1 Lift the Mini8 Plus instrument out of the package Place it on a flat surface and remove the packaging materials Keep the box and packaging in case of a return 2 Check the packing list as follows to ensure that ...

Страница 4: ...arted running to set up the environment as follow Click Next PC port Mini8 Plus port A B C Power cable USB cable Power adapter A B C Select DriverInstaller Application NET Framework 4 0 NI LabVIEW Runtime 2014 SP1 f3 and Click next Click next per dialog box continuously to install DriverInstaller Application NET Framework 4 0 NI LabVIEW Runtime 2014 SP1 f3 Click Install Click OK Click Next 4 5 ...

Страница 5: ...rating environment and check the installation options If a project has been installed you can manually cancel Click next to start the installation Note During the installation the system may be requested to restart you can choose to restart later Click Next Click Next 6 7 ...

Страница 6: ...ation path and click Next to continue Click Next 2 The software installation will be boot up once the environment detection completed Click Install to start the installation The Mini8 Plus software is installing 8 9 ...

Страница 7: ... wells 6 Start the run The Monitor Run tab opens Do not open the lid while a run is in progress This will corrupt the data 7 When the run is completed open the Mini8 Plus lid Remove the strip from the block Dispose of any hazardous materials into appropricate containers for biohazard caustic materials according to your local safety regulations Load reaction tubes 1 Thaw all necessary reagents temp...

Страница 8: ...ick OK Warning Do not touch hot lid The hot lid temperature would be up to 105 C 221 F when the device is working 2 Fill in your Email when the running is over the result report Excel file will be sent to your email PS The computer need to be connected to the network 1 If you want to pause running when Rn reaches the setting value please input terminal Rn for each sample This function can help you...

Страница 9: ...ollowing steps 1 Click the Plate Setup window opens 2 Click to choose the sample 3 Set up sample name 4 Set up sample type unknown positive control negative control standard When the sample type is defined as Standard the concentration setting interface will be promoted for user to set the Standard concentration in the following interfaces Define the Plate Layout Define Sample Name Define Sample T...

Страница 10: ...rag Bar Up or Down to Adjust Temperature and Duration Click Temperature Plateau to Adjust Temperature and Duration Remaining Time Warning Do not open the lid while a run is in progress It may allow extraneous light enter the system so the data will be corrupted Note If you do not set up the fluorophore channel when set the thermal profile there will be not any curve showing neither during nor afte...

Страница 11: ...Data Analysis Click to show the saved data CH1 FAM CH2 ROX Thermal Profile PCR amplification curve PCR amplification curve Melt Peak Melt Curve 18 19 ...

Страница 12: ...strument Lights System Information Lights The Mini8 Plus System has four indicator lights on the bottom right corner of the top panel Power Error Status and Scaning The following table shows the meaning of each combination of off on and flashing lights Description Power off Standby mode Power on PCR is running PCR is running scanning Fatal Error instrument might be overheated 20 21 ...

Страница 13: ...nal RF sources as these may interfere with proper operation If you notice any interference discontinue using the system until all issues are resolved Resolution may include moving cords from other equipment away from the system plugging the system into an outlet on a different circuit from other equipment or moving the system away from other equipment If you still have difficulties contact COYOTE ...

Страница 14: ... 3x109 bp x 618g mol bp 6 02x1023 3 08x10 12 g One haploid cell sperm egg 3 08 pg of DNA One diploid cell 6 16 pg of DNA RNA concentration μg μl A260 40 D 1000 where D dilution factor and A260 absorbance at 260 nm DNA concentration μg μl A260 50 D 1000 where D dilution factor and A260 absorbance at 260 nm 24 ...

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