Consort E3100, Руководство пользователя

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E3xxx E3xxx
Gel pouring Gel pouring with casting dams with casting dams Gel pouring Gel pouring Gel pouring Gel pouring
1
. Fit the casting dams over each end of the tray and place onto
a level surface. The dams should be fitted so that there is no
gap between the sides of the tray and the groove in the dams.
This will ensure that there is no possibility of gel leakage.
2 . Place the comb(s) in the grooves. Each tray has more than
one comb grove so that multiple combs can be used. Using
multiple combs increases sample number available per gel
but decreases run length and care must be taken to ensure
that samples from the first wells do not migrate into the lanes
of the second comb wells.
3 . Pour in the agarose carefully so as not to generate bubbles.
Any bubbles that do occur can be smoothed to the edge of the
gel and dispersed using a pipette tip.
4
. Allow the agarose to set, ensuring that the gel remains undis-
turbed.
5
. Carefully remove the gel casting gates and comb and transfer
the gel including tray to the main tank. the gel including tray to the main tank.
Gel pouring Gel pouring with model E3100 with model E3100 Gel pouring Gel pouring Gel pouring Gel pouring
1. Fit the casting gates into the grooves in the unit which are 10
mm from the platinum wire. Ensure that these are seated as
deeply as possible. This will ensure that a good seal is formed
and that there is no possibility of gel leakage. A small amount
of vaseline or sealing grease on the bottom and side edges of of vaseline or sealing grease on the bottom and side edges of
the gates will give further leakage protection.
2 . Place the comb(s) in the grooves. Each tray has more than
one comb grove so that multiple combs can be used. Using
multiple combs increases sample number available per gel
but decreases run length and care must be taken to ensure
that samples from the first wells do not migrate into the lanes
of the second comb wells.
3
. Pour in the agarose carefully so as not to generate bubbles.
Any bubbles that do occur can be smoothed to the edge of the
gel and dispersed using a pipette tip.
4
. Allow the agarose to set, ensuring that the gel remains undis-
turbed.
5 . Carefully remove the gel casting gates and comb and transfer
the gel including tray to the main tank.