Consort E3100, Руководство пользователя

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E3xxx E3xxx
7
Gel pouring Gel pouring using tape method using tape method
1
. Autoclave or plastic backed general tape should be used. A
length 5 cm longer than the width of each end of the tray
should be cut. One length should be placed over one end of
the tray folded and the edges sealed securely. Repeat for the
other end and place onto a level surface for gel pouring.
2 . Place the comb(s) in the grooves. Each tray has more than
one comb grove so that multiple combs can be used. Using
multiple combs increases sample number available per gel
but decreases run length and care must be taken to ensure
that samples from the first wells do not migrate into the lanes
of the second comb wells.
3 . Pour in the agarose carefully so as not to generate bubbles.
Any bubbles that do occur can be smoothed to the edge of the
gel and dispersed using a pipette tip.
4
. Allow the agarose to set, ensuring that the gel remains undis-
turbed.
5
. Carefully remove the gel casting gates and comb and transfer
the gel including tray to the main tank. the gel including tray to the main tank.
Gel pouring using the flexicaster Gel pouring using the flexicaster
1 . Level the fl exicaster base by adjusting the feet so that the
bubble is exactly central.
2 . Insert the desired length tray into the fl exicaster such that
one end of the tray is pushed up and seals against the silicone
mat of the permanent end of the fl exicaster.
3
. Position the movable end of the fl exicaster so that the sili-
cone mat is pushed against the other end of the tray.
4
. Turn the cam so that the silicone mat tightly seals against
the side of the tray. Pour in the agarose carefully so as not to
generate bubbles. Any bubbles that do occur can be smoothed
to the edge of the gel and dispersed using a pipette tip.
5 . Allow the agarose to set, ensuring that the gel remains undis-
turbed.
6 . Carefully remove the gel casting gates and comb and transfer
the gel including tray to the main tank.