Continue by adding further steps and stages as required in the same way.
Note:
If a protocol has several stages it may appear to extend beyond the screen; simply swipe the screen
to the left or the right to view the whole protocol.
To adjust the number of cycles of a stage, tap on the cycle button at the bottom of each stage area; a
scrolling selector will appear. Select the desired number of cycles followed by
Change
.
As the program is written, the total duration of the protocol is displayed at the bottom of the programming
screen.
EDITING STEP PARAMETERS
To edit the parameters of a step e.g. the temperature, hold time, ramp rate etc. tap on the grey shaded
area of the step. The
Change step parameters
window (see below) will open. Make any changes to
the parameters then tap on
Change
to accept.
Note:
The ramp rate (heating/cooling rate from the previous step to the current step) can be defined
between 0.1 ºC/sec up to 3 ºC/sec or MAX.
ADDING A GRADIENT STEP
Select the step on which you wish to add a gradient and tap on the grey shaded area. The
Change
step parameters
window will open. Toggle
ON
the parameter
Gradient around step temperature
.
Next, define the range of temperature you wish to run the gradient over. Tap on
Change
to accept the
changes.
The gradient and range will then be displayed within the program.
35
Содержание PCRmax Alpha Cycler 1
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