a
b
c
11.3 Supplementary Manual for Polarization Model 78904-33 (cont.)
Adjustments before observation
Centering the objectives
All 360° centering objectives of the MSU-600 Series material microscopes are pre-
centered in our factory. However, during transportation or after a long period of inactivity,
the centering of these objectives may have been shifted.
Please follow the steps below in order to re-center the objectives of a polarization
microscope:
1.
Remove one eyepiece from the microscope head
2.
Insert the widefield eyepiece with a crosshairs into the tube of the eyepiece you just
removed
3.
Position a microscope slide with a crosshairs reticule under the stage clamps
4.
Check if the 4x objective is positioned in the optical path
5.
Position the round stage with the vernier on its “0” position
6.
Position the middle of the crosshairs of the microscope slide on top of the cross-
hairs of the eyepiece (a)
7.
Rotate the stage by 180°. A displacement might be observed (b)
8.
Move by hand, the middle of the crosshairs of the microscope slide, approximately
half way to the eyepiece crosshairs (c)
9.
Rotate the stage back to its “0” position
10.
The 4x objective is equipped with two adjustable screws inside the revolving
nosepiece for centering the objective. Use the centering screws to move the center
of the eyepiece crosshairs towards the center of the crosshairs of the microscope
slide
11.
Repeat steps 7 to 10 until the objective is centered
12.
Repeat for the other objectives using same procedure
If the centering cannot be done correctly:
1.
Check if the 4x objective is set in the central position of the centering screw’s correction range. This means that
when the centration is changed by using the screws in the revolver, the cross of the slide should be able to move
to all directions in an equal amount
2.
Repeat this for the other objectives
3.
If the centering is still unsuccessful, please check if the mechanical stage is correctly centered. The stage is fixed
by four screws at the bottom of the stage. Untighten the screws so the stage can be moved and align the stage
around the lens of the condenser visually. Note that the condenser should first be aligned in the correct manner,
the procedure is described in the general MSU-600 Series microscope user manual
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