Cleaver Scientific CSL-CELLAS Скачать руководство пользователя страница 7

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27/05/2020

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Place the strip on top of the wicks and between the bars with the

sample end at the negative electrode. Make sure there is good con-

tact by pushing down on the ends of the strip. Glass slides can be

placed over each end to further ensure a good contact.

Apply the samples at the negative end of the cellulose acetate

plate. Usually a small amount of dye can also be loaded to indicate

sample migration distance. The samples should be kept as cold as

possible before application to prevent denaturation.

Place the safety lid onto the tank and connect the leads to a power

supply.

Electrophorese at 200 volts for 15

–

30 minutes or following cellulose

acetate strips set up.

10.

Once electrophoresis is complete, the strip can be stained.

Содержание CSL-CELLAS

Страница 1: ...CELLASMINI Record the following for your records Model _____________________ Catalogue No _____________________ Date of Delivery _____________________ Warranty Period _____________________ Serial No _...

Страница 2: ...ng List 4 Operating Instructions 5 Usage Guidance and restrictions 5 Setting up the Cellulose Acetate Tanks 5 Fitting Electrode cables 5 Preparation and Gel running 6 Example of Staining 8 Example of...

Страница 3: ...e complete manual thoroughly The unit must never be used without the safety lid correctly in po sition The unit should not be used if there is any sign of damage to the exter nal tank or lid These uni...

Страница 4: ...units are received to ensure that all components have been included The unit should be checked for damage when received Cleaver Scientific is liable for all missing or damaged parts accessories within...

Страница 5: ...owever a temporary conductivity caused by condensation must be expected Setting up the Cellulose Acetate Tanks Fitting Electrode cables Note the position of the lid on the unit This shows the correct...

Страница 6: ...sition the electrode wicks in the tank so that they overhang the wick carrying bars with the base sit ting in the buffer tanks see diagram below Filter paper or paper towels can be used as the wicks 3...

Страница 7: ...ply the samples at the negative end of the cellulose acetate plate Usually a small amount of dye can also be loaded to indicate sample migration distance The samples should be kept as cold as possible...

Страница 8: ...hly rinsed with warm water or distilled water to prevent build up of salts but care should be taken not to damage the en closed electrode and vigorous cleaning is not necessary or advised Air drying i...

Страница 9: ...ent as described above Wash with 3 hydrogen peroxide H2O2 for 10 minutes Rinsed with 0 1 DEPC diethyl pyro carbonate treated distilled water Caution DEPC is a suspected carcinogen Always take the nece...

Страница 10: ...ne other than CSL or an appointed distributor or representative are no longer under warranty from the time the unit was modified Units which have accessories or repaired parts not supplied by CSL or i...