ChemoMetec NucleoCounter NC-202, Компиляция документации

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Doc.No: 994-2026 v. 1.1 · Issue date: 02-Oct-2019
∙ Template: 880-0011-79A v.3
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ChemoMetec A/S · Gydevang 43 · 3450 Alleroed · Denmark · support@chemometec.com · www.chemometec.com ·
Troubleshooting
Inaccurate cell count (my cell count is either too high or low):
When analyzing a new cell line, it is important to verify that the cells are correctly identified. Cells identified by
NC-View
™ can be shown by clicking cell overlay, right panel (Figure 1). All cells should be highlighted, while cellular
debris should be excluded.
Imprecise cell count (I have a large variation between technical replicates):
The cell counting precision, often quantified as Coefficient of Variation from replicate counts, are affected by many
variables. 1) Cell concentration: A low cell sample concentration will negatively affect the precision. See Technical note:
Variation and Statistics . 2) Liquid handling: The cell suspension should be thoroughly mixed before the sample is
collected with the Via 2 -Cassette ™ . 3) The cassette can load from 200 µl sample in a 1.5 ml tube, however increasing the
sample volume improves the precision. 4) Consistent protocol execution. 5) Sample preparation: Ensure that cell
sampling and sample dilutions are made with wide orifice tips, to avoid 'bottleneck effects'.
Handling and storage
For handling and storage of ChemoMetec® instruments and
reagents, cassettes and NC-Slides refer to the corresponding
product documentation. For other reagents refer to the
material data sheet from the manufacturer of the reagents
and chemicals.
Warnings and precautions
For safe handling and disposal of the ChemoMetec®
reagents, cassettes and NC-slides refer to the corresponding
product documentation and the NucleoCounter® NC-202

user guide. For other reagents refer to the safety data sheet
from the manufacturer of the reagents and chemicals
required for this protocol. Wear suitable eye protection and
protective clothes and gloves when handling biologically
active materials.
Limitations
The NucleoCounter® NC-202

system is FOR RESEARCH USE
ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE. The
results presented by the NucleoCounter® NC-202

system
depend on correct use of the reagents, Cassettes and the
NucleoCounter® NC-202

instrument and might depend on
the type of cells being analyzed. Refer to the
NucleoCounter® NC-202

user’s guide for instructions and
limitations.
Liability disclaimer
This application note is for RESEARCH PURPOSES ONLY. It is
not intended for food, drug, household, or cosmetic use. Its
use must be supervised by a technically qualified individual
experienced in handling potentially hazardous chemicals.
The above information is correct to the best of our
knowledge. Users should make independent decisions
regarding completeness of the information based on all
sources available. ChemoMetec A/S shall not be held liable
for any damage resulting from handling or contact with the
above product.
Product disclaimer
ChemoMetec A/S reserves the right to introduce changes in
the product to incorporate new technology. This application
note is subject to change without notice.
Copyright
Copyright © ChemoMetec A/S 2019. All rights reserved. No
part of this publication and referred documents may be
reproduced, stored in a retrieval system, or transmitted in
any form or by any means, electronic, mechanical,
photocopying, recording or otherwise, without the prior
written consent of ChemoMetec A/S, Gydevang 43, DK-3450
Allerod, Denmark.
ChemoMetec and NucleoCounter are registered trademarks
owned by ChemoMetec A/S. Via2-Cassette and NC-View are
trademarks of ChemoMetec A/S. All other trademarks are
the property of their respective owners.
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