Set Run Time, then press ENTER. (2:30 is recommended but may be adjusted).
1.1.7.
Set Hold Time, then press ENTER. (15:00 is recommended but may be adjusted.)
1.1.8.
Set Stirring to “OFF”, then press ENTER.
1.1.9.
Set Cooling to “OFF”, then press ENTER.
1.1.10.
Set “Next Stage = (N)”, then press ENTER.
1.1.11.
Set “Save Method = (Y)”, then press ENTER.
1.1.12.
Create a method name using the arrow keys, highlight “Exit”, and press ENTER. The
1.1.13.
method is now saved in the software.
To use an existing method:
1.2.
Press the Open Folder key.
1.2.1.
Use the right arrow key to scroll to the desired method name, then press ENTER.
1.2.2.
Set up the vessel.
2.
Slide the thermowell (closed end first) though the top of an open ferrule nut until the top of the
2.1.
thermowell is even with the top of the nut (Figure 2, center picture).
Pass the closed end of the thermowell through the center port of the 2-port vessel cap until
2.2.
the nut meets the threaded port. Tighten the nut finger tight onto the threaded port to create a
secure seal (Figure 2, right picture). Do not use a wrench, as this will damage the threads.
Add the protein solution to the microvials and dry using a vacuum centrifuge. Using forceps,
2.3.
insert the vials to the microvial insert tray.
Add 10 mL of 6N HCl with 1% Phenol to the vessel body.
2.4.
Place the insert tray into the vessel body. Fit the condensation shield over the vials (Figure 3).
2.5.
Figure 2. Thermowell Assembly.
Figure 3. Microvial Loading.
NOTE
Run Time is the time it takes for the Discover to reach the maximum temperature. The Hold Time is the
time the Discover maintains the maximum temperature.
