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Fumigation & Formalin Quantities – 2.5
When handling hazardous materials the air space inside the cabinet should be decontaminated
regularly and always before servicing and following any spillages. Fumigation by formaldehyde
gas is the recommended decontamination procedure for biological hazards although there are
alternative methods available including VHP decontamination and Ozone decontamination.
To facilitate the fumigation a sequence has been incorporated in the cabinet controls, this is
detailed under fumigation procedure.
A convenient way of generating sufficient formaldehyde is to boil off Formalin (40%
formaldehyde BP or equivalent) in a suitable vessel such as a formalin vaporiser. These are
available from CAS.
Formalin Quantities
The recommended quantity stated in BS EN12469:2000 on Page 40 Annex J, Part 2 is 60ml
formaldehyde solution mixed with 60ml distilled water per cubic meter of cabinet volume.
However this quantity is now considered in excess of that required to achieve a satisfactory kill.
We have therefore produced the following table based on quantities employed by users of
large numbers of Safety Cabinets.
If you still consider that the quantities recommended in the British Standard are to be used,
you may find on completion of the sterilisation cycle that high quantities of fluids containing
formaldehyde are present in the cabinet.
Cabinet Size 900mm 1200mm 1500mm 1800mm
Formalin 15ml @40% 20ml @ 40% 25ml @ 40% 30ml @ 40%
Distilled Water 15ml 20ml 25ml 25ml
Always consult your Laboratory Safety Officer
prior to fumigation of a safety cabinet.
** If in doubt ask **