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Cleaning Procedure – 2.4

Regular cleaning is very important to prevent the build-up of dirt and hence potentially
infectious material. Routine swabbing of work surfaces with 70% v/v IMS (ethanol) or IPA
(Isopropyl Alcohol) is recommended.

For cleaning the work surfaces, swabbing with a mild detergent in warm water is very
effective. Phenolic or Cresolic disinfectants should be avoided as they may stain the white
surfaces with a brownish colour. If they are used, any spillage should be quickly rinsed with
clean water and mopped up with an absorbent tissue. Most of the quaternary ammonium
compounds and the Glutaraldehyde based surface disinfectants are suitable.  

To facilitate cleaning of the work zone and the interior, the whole front screen / visor may be
opened and lowered from the top, supported by the hinges at the lower edge. It is good
practice to clean the inside of the viewing screen to ensure adequate visibility of the working
zone. Always consult the Laboratory Safety Officer before carrying out this procedure.

      

   Warning

If Hypochlorites are used to clean the stainless
steel interior of the safety cabinet they will
initially cause rust spots and over time may lead
to further damage.

Содержание BioMAT 1

Страница 1: ...BioMAT 1 Safety Cabinet ...

Страница 2: ...rt Up Procedure Shut Down Procedure Cleaning Procedure Fumigation Formalin Quantities Fumigation Procedure Airflow Measurements Ultraviolet Radiation Section 3 Cabinet Control Panel 3 1 3 2 3 3 3 4 3 5 Display Alarm Circuits Electrical Protection Fan Speed Control Engineers Menu Section 4 Filter Replacement 4 1 4 2 4 3 Re circ HEPA Filters Exhaust HEPA Filter General Notes Section 6 Service Spares...

Страница 3: ... filtration HEPA prior to being dispersed back into the laboratory or to atmosphere Operator protection is provided by a constant inflow of air through the working aperture of the front screen The Class 1 Microbiological Safety Cabinet can be supplied as standard in either of the following two modes Ø Re circulating Type Exhaust air from the safety cabinet is passed through two high efficiency fil...

Страница 4: ...ained personnel or approved CAS agents Ø Extension modification relocation repairs or other maintenance is carried out by CAS personnel or persons authorised by CAS or in the case of electrical work by qualified electricians Ø In the case of repair or maintenance replacement parts supplied by CAS must be used Ø The electrical installation surrounding the unit and to which it is connected comply wi...

Страница 5: ...ints are of particular relevance to class 2 safety cabinets The diagram shown below indicates some suggested locations for the correct siting of safety cabinets and highlights some situations which should be avoided 3 4 2 1 HORIZONTAL LAMINAR FLOW SUPPLY GRILLE Position 1 An acceptable site not affected by disruptive air currents Position 2 Well sited Position 3 Poorly sited if windows open If not...

Страница 6: ...d have a maximum velocity of no more than 0 30m sec Commissioning When any safety cabinet is installed it is necessary to carry out a number of commissioning checks in order to ensure it is fully operational and that the performance on site satisfies the current standard BS EN 12469 2000 This includes measuring the airflows testing the HEPA filters with a suitable challenge aerosol and a KI Discus...

Страница 7: ...et operator and safety cabinet performance 1000 Keep pedestrians away from the front of your safety cabinet Keep clear of adjacent wall 300 Position well away from the door openings 1500 1500 Position clear of bench opposite Keep clear of structural columns Keep well away from opposite wall 2000 Bench C 300 ...

Страница 8: ...ical Exhaust Clean Filters Pa 350 350 350 350 Exhaust Dirty Filters Pa 500 500 500 500 HEPA Filter Data HEPA Type H14 EN1822 H14 EN1822 H14 EN1822 H14 EN1822 Efficiency 0 3µ 99 999 99 999 99 999 99 999 Heat Gains Typical Exhaust Watts 150 150 220 250 Re circ Watts 150 150 220 250 Noise Typical Noise Level dB A 58 58 58 58 Light Typical Lighting Level White Lux 900 900 900 900 Lighting Level St St ...

Страница 9: ...instruction It is intended to supplement existing in house procedures and codes of practice and not to replace them If further advice is required on the use or maintenance of this equipment the staff of Contained Air Solutions Ltd will be pleased to assist wherever possible Switches Indicators 2 1 ...

Страница 10: ...ow Controlling power to lights C Gas Valve Optional Black To activate solenoid Gas Valve Will only function when cabinet is in safe working condition D Alarm Mute Red To mute audible alarm Fault indication on display will remain until fault is rectified E Fumigate Orange To activate fumigation cycle Will only operate when cabinet is switched OFF F UV Lights Optional Blue To activate power to UV Li...

Страница 11: ...ch A on the control panel this will energise the exhaust fan The main display will show AIRFLOW STABILISING for 60 seconds once airflows are settled the display will show AIRFLOW SAFE On start up the audible alarm will sound until airflows are safe this may be muted using the Alarm Mute switch D NOTE Should the airflows change significantly and fall out of the specifications laid down in EN12469 2...

Страница 12: ... cleaned in accordance with laboratory codes of practice The cabinet should be left running for a few minutes to clear any residual aerosols Ø Switch off the interior light using switch B Ø Switch off the cabinet using switch A Ø Place the closure panel Night door over the working aperture ...

Страница 13: ... with clean water and mopped up with an absorbent tissue Most of the quaternary ammonium compounds and the Glutaraldehyde based surface disinfectants are suitable To facilitate cleaning of the work zone and the interior the whole front screen visor may be opened and lowered from the top supported by the hinges at the lower edge It is good practice to clean the inside of the viewing screen to ensur...

Страница 14: ... These are available from CAS Formalin Quantities The recommended quantity stated in BS EN12469 2000 on Page 40 Annex J Part 2 is 60ml formaldehyde solution mixed with 60ml distilled water per cubic meter of cabinet volume However this quantity is now considered in excess of that required to achieve a satisfactory kill We have therefore produced the following table based on quantities employed by ...

Страница 15: ...ng a lightweight closure panel additional sealing tape will be required around the outside face to ensure no leakage during fumigation Now press the fumigation button E and the display will show FUMIGATION IN PROGRESS A countdown timer will be shown directly below in minutes e g 285 MINS Warning Ø The cabinet should be left for a minimum of 6 hours preferably overnight Note Once the fumigate butto...

Страница 16: ...eres to the surfaces of the cabinet and within the media of HEPA filters we recommend that the cabinet be run for between 4 and 6 hours before the cabinet is serviced or work re commences Ø Remove the optional fumigation kit from the cabinet discharge before commencing to use the cabinet Any poly formaldehyde residue in the vaporiser may be removed by heating with water containing a little mild de...

Страница 17: ... take 5 readings namely one in each corner and one in the centre on the work area aperture Readings should be not less than 0 70m sec or greater than 1 0m sec Readings above 1 0m sec may cause problems with containment this may be checked by conducting a KI test Anemometer positions for the 1200 1500 1800mm size of cabinets are illustrated below The results should be recorded to monitor cabinet pe...

Страница 18: ...bes is known to reduce therefore we would recommend UV tubes are replaced on an annual basis to ensure maximum efficiency Applications Many bacteria are quite resistant to UV Radiation and may require prolonged exposure for sterilisation Dry and or protein covered organisms may be protected against UV and may be only slightly affected if at all However moist vegetative cells without too much prote...

Страница 19: ...ion shown below AIRFLOW SAFE BioMAT 1 In the event of an alarm condition the display will clearly indicate the fault this will remain displayed until fault is rectified Typical display during alarm condition shown below LOW AIRFLOW Should the cabinet produce an alarm condition it may be necessary to arrange for a Service Engineer to attend site in such cases please contact our Service Department o...

Страница 20: ...it In the event the alarm is triggered the display will show HIGH AIRFLOW and the audible alarm will sound this can be muted using the alarm mute button 3 FRONT SCREEN Front screen position is monitored by a micro switch fitted to the front screen assembly When opened the display will show VISOR OPEN and an audible alarm will sound This can be muted using the alarm mute button The alarm circuit is...

Страница 21: ...nt control panel which is secured with two screws located across the bottom edge Once removed the control panel will hinge upwards to facilitate access to the main printed circuit board For full details on fuse ratings and the circuits they protect please see the wiring diagram attached to this manual Ensure the Safety Cabinet is isolated from the mains supply prior to opening access panels ...

Страница 22: ...lanned maintenance it may be necessary to increase cabinet fan speeds to overcome filter soiling This can be adjusted via the front control panel membrane using the Engineer s Menu Warning It is important any changes to fan speeds must be made by a CAS service engineer or alternative competent service provider failure to do this may result in the warranty being invalid ...

Страница 23: ...tched OFF STANDARD SWITCH PANEL ARRANGEMENT SOFT BUTTON ARRANGEMENT TO ENTER ENGINEERS MENU BUTTON 1 SHOULD HELD DOWN FOR 5 SECONDS USE BUTTON 2 TO START STOP THE FANS TO EXIT ENGINEERS MENU BUTTON 1 SHOULD HELD DOWN FOR 5 SECONDS It is important any changes within the Engineer s Menu be conducted by a CAS service engineer or alternative competent service provider failure to do so may result in th...

Страница 24: ...Sequence LOWALARM 40 Pa YES NO HIGH ALARM 80 Pa YES NO INFLOWFAN 65 YES NO U V LIGHTING 060 MINUTES YES NO TIME ADJUST 14 54 YES NO EXHAUSTSENSOR 20 Pa YES NO SERVICETIMER RESET YES NO INITIALISEVALUE INITIALISE 0 YES ...

Страница 25: ...o the supplier immediately Slide filters and spacer carefully into position Tighten filter bolts ensuring filters and spacer section are correctly positioned and gaskets are compressed to about half their original thickness The filters and their gaskets should be tested for leaks by challenging with DOP or equivalent aerosol and scanning the surfaces with the probe of a forward light scattering ph...

Страница 26: ... Slide filter carefully into position Tighten bolts ensuring filter is correctly positioned and gaskets are compressed to about half their original thickness The filter and its gaskets should be tested for leaks by challenging with DOP or equivalent aerosol and scanning the surface with the probe of a forward light scattering photometer Ensure the Safety Cabinet is isolated from the mains supply p...

Страница 27: ...tch on the control panel marked gas valve Ø As a safety feature the UV tubes If fitted are interlinked with the cabinet lights to prevent them being used when the cabinet is in use We recommend cabinet users to consult their own laboratory safety information Other publications available include Science Reviews Ltd Occupational Hygiene Monograph No 9 British Standard BS EN 12469 2000 5726 2000 Part...

Страница 28: ...and electronic controls as related to the airflow system 6 Check and adjust operation of exhaust system as required 7 Mechanically inspect cabinet components and duct connections if fitted 8 Change main filters as necessary Supply and fitting subject to additional charge 9 Prepare computer generated Service Report Above work to be carried out twice each service year Operator Protection Test K I Di...

Страница 29: ...61 655 8865 Fax 44 161 655 8865 ORDERING CODES Item BioMAT 1 1200mm BioMAT 1 1500mm BioMAT 1 1800mm Exhaust HEPA Filter x 1 off FIL 161 FIL 021 FIL 144 Recirc HEPA Filters x 2 off FIL 161 FIL 021 FIL 144 Exhaust Fan FAN 050 FAN 057 FAN 057 Main PCB ELC 325 ELC 325 ELC 325 Control Membrane LAB 114 LAB 114 LAB 114 Light Tube ELC 077 ELC 097 ELC 049 UV Light Tube ELC 061 ELC 061 ELC 061 Key Switch EL...

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