Brooks CryoPod, Быстрый старт

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Step 2: Manual LN2 Filling
Steps LCD Display Photo
1.Ensure the unit is powered OFF and cryogenic
chamber basket is empty. N/A
2. Insert funnel into the front left corner of the unit.
Ensure funnel is fully inserted and firmly seated to
avoid tipping. N/A
3. Insert the fill sensor in the back right corner
diagonally opposite the funnel. The notched
underside should line up with chamber basket edge.
Sensor should be fully lowered. N/A
4. Fill a Dewar flask with liquid nitrogen.
Approximately 3L in total will be needed. N/A
5. Slowly pour ~2L LN2 into the funnel, ensuring that
the funnel is never more than half full at all times.
Stop pouring when the sensor rod starts to bob up
and down and the colored fill line is visible. N/A
6. Turn ON the unit by pressing the Power button (A).
7. Wait approximately 15 minutes until the
temperature reaches -150°C. LCD will reflect
cooling chamber
temperature
8. Slowly pour additional ~1L of LN2 into the funnel,
ensuring that the funnel is never more than half full
at all times. Stop pouring when the sensor rod stops
bouncing and the bottom of the colored fill bar is
visible.
9. Remove funnel and fill sensor using cryogenic
gloves. Place lid on unit. Allow 10-15 minutes for
chamber temperature to equilibrate. When the
CryoPod
™ Carrier reaches -170°C or below, the unit is
ready for use.
LCD will reflect
chamber temperature
< -170°C
General Safety
CryoPod
™
Carrier is intended for the exclusive use by trained and experienced laboratory
personnel. CryoPod
™
Carrier users are advised to comply with applicable organizational
standard operating procedures, industry safety guidelines, and local, regional, national and
international laws and regulations.
Please adhere to all safety guidelines listed in the CryoPod
™
Instructions for Use.
Troubleshooting: Service and Error Codes