- 12 -
- 13 -
Incident light illumination is optimised for the 4x objective. The
specimen is generally no longer fully illuminated if the 10x or
40x objective is used.
5. Viewed Object – condition and preparation
Both transparent and non-transparent specimens can be
examined with this microscope, which is a direct as well as
transmitted light model. If opaque specimens are examined
- such as small animals, plant parts, tissue, stone and so
on - the light is reflected from the specimen through the lens
and eyepiece, where it is magnified, to the eye (reflected
light principle, switch position I). If opaque specimens are
examined the light from below goes through the specimen,
lens and eyepiece to the eye and is magnified en route (direct
light principle, switch position II). Many small organisms of the
water, plant parts and finest animal components have now
from nature these transparent characteristic, other ones must
be accordingly prepared. Is it that we make it by means of a
pre-treatment or penetration with suitable materials (media)
transparent or thus that we cut finest wafers off of them (hand
cut, MicroCut) and these then examine. With these methods
will us the following part make familiar.
6. Creation of thin preparation cuts
Specimens should be sliced as thin as possible, as stated
before. A little wax or paraffin is needed to achieve the best
results. A candle can be used for the purpose. The wax is put
in a bowl and heated over a flame.
DANGER!
Be extremely careful when working with hot wax.
There is a risk of burns.
The specimen is then dipped several times in the liquid wax.
The wax is finally allowed to harden. Use a MicroCut or knife/
scalpel (carefully) to make very thin slices of the object in its
wax casing.
DANGER!
Be extremely careful when using the knives/
scalpels or the MicroCut. There is an increased risk
of injury due to the sharp edges!
These slices are then laid on a glass slide and covered with
another.
7. Creation of an own preparation
Put the object which shall be observed on a glass slide and
give with a pipette (Fig. B 1) a drop of distilled water on the
object (Fig. B 2).
Set a cover glass (in each well sorted hobby shop available)
perpendicularly at the edge of the water drop, so that the water
runs along the cover glass edge. Lower now the cover glass
slowly over the water drop.
8. Gum Media
Gum media can be used to embed or preserve specimens.
Warm the gum media slightly in a water bed to make it easier
to use. It is used similarly to how water is used when preparing
a specimen (image B1), except that a dissecting needle is used
in this case. Vaseline or a clear nail varnish, for example, can
also be used to preserve specimens.
9. Connection to a PC/laptop
Connect the USB cable to the socket (Fig. 1, image 13) on the
microscope and a free USB port on your PC.
Press „MSCD“ to transfer images directly from the SD card.
Press „PC camera“ to transmit the live image from the camera.
The resolution corresponds to the video resolution (Fig. 3,
1.2.2. Installation of the Image Editing Software Photomizer
The image editing software “Photomizer” is located on the
software CD. You can edit your pictures here.
1. To install the software, click once on the menu point
“Install Photomizer” with the left mouse button.
2.
The Photomizer Software requires Microsoft .NET
Framework 3.5, which it will install if it is not already on
your system. If it is already on your system, you may skip
to step 5.
3.
In the welcome window, please accept the Microsoft
license agreement, and then click “Install”. The installation
can take a few minutes.
4. As soon as everything is installed, click “Finish”.
5. Now you will be presented with a choice, in which you can
choose your language.
Make your selection and confirm it by clicking „OK“.
6. When you see “Welcome“, click on „Next“.
7. In the next window, you will be asked for the „Destination
Folder“. Here, just click on „Next“.
8. The „Ready to install Photomizer“ window appears.
Select whether a shortcut should be created on the
desktop or the quick start bar.
9. Now, the window with the Setup-Status will appear –
here, a progress bar will inform you about the ongoing
installation. This process can take a few minutes.
10.
The window “Photomizer is being installed” appears.
Click on “Finish”. The installation ends.
1.3. Troubleshooting: the device isn‘t recognised by the
computer (USB)
Please contact our Service: [email protected]
2. Electric LED illumination with dimmer
Transmitted light illumination (Fig. 1, image 6) is used for
transparent specimens (specimens on microscope slides).
Select incident light illumination (Fig. 2, image 15) to observe
opaque objects. The simultaneous use of transmitted
and incident light illumination is only advisable with semi-
transparent objects. This mode is not recommended for
transmitted light objects on slides since too much light may be
reflected on the slide.
3. Colour filter/aperture combi wheel
The colour filter/aperture combi wheel (Fig. 1, image 8) beneath
the microscope table can be used to influence the image
quality when observing transparent specimens. Fine details
are displayed better depending on the colour and object. A
coloured lower light in combination with a white upper light
can also optimise image details when observing objects under
incident light (e.g. transparent objects).
Depending on the aperture used, the corresponding light
bundling can also influence the sharpness, depth of field,
contrast and detail resolution during observation.
4. Observation
Turn the nosepiece (Fig. 1, image 3) to select the 4x objective
for observing objects. Depending on the object, select upper
or lower lighting for optimum illumination. Place a specimen
on the microscope table (Fig. 1, image 5) directly under the
objective and secure it using the slide clips (Fig. 2, image 16).
Important: The object to be observed must lie precisely in the
beam path of the transmitted light illumination. Now slowly
turn the focusing wheel (Fig. 2, image 10) until the image on
the monitor is reproduced sharply. The dimmers (Fig. 2, image
17) and (Fig. 2, image 18) can be used to optimally adjust
the contrast and quality of the image of the object under
observation.
Turn the nosepiece (Fig. 1, image 3) to switch objectives if
increased magnification is desired. Incidentally: The higher
the level of magnification, the more light is required for good
image quality.
4. Cleaning optical parts:
In order to enhance the optical quality, the lenses of
eyepieces or objectives are coated. They should not be
wiped because dry dirt or dust may scratch the coating. It is
best to remove the parts to be cleaned from the frame prior
to cleaning. Always blow loose dust away first. Use lens
tissue of good quality or soft fabric moistened with a small
amount of pure alcohol (available in the chemist’s shop);
wipe the lens surface clean.
5. When not in use:
After usage, cover the microscope with the dust cap (made
of plastic) and place it where it is dry and mouldless. In
the case of non-use for a longer time, you should stow the
microscope and the accessories in their correct containers
again. We suggest the storage of all objectives and
eyepieces in a closed container with drying agent.
Remember: A well maintained microscope will keep its
optical quality for years and thus maintain its value.
DISpoSAl
Dispose of the packaging materials properly,
according to their type (paper, cardboard, etc).
Contact your local waste disposal service or environmental
authority for information on the proper disposal.
Do not dispose of electronic devices in the household
garbage! As per the Directive 2002/96/EC of the
European Parliament on waste electrical and electronic
equipment and its adaptation into national law, used electronic
devices must be collected separately and recycled in an
environmentally friendly manner.
Please take the current legal regulations into account when
disposing of your device. You can get more information on
the proper disposal from your local waste disposal service or
environmental authority.
image G). In order to transmit a live image, it is necessary to
install the drivers and software contained on the CD provided.
10. The lifecycle of the saltwater prawn
The saltwater prawn or artimia salina to scientists has an
unusual and interesting lifecycle. The female‘s eggs are
hatched without any male shrimp having to fertilise them.
The resultant baby prawns are all female. Under unusual
circumstances such as when a swamp is drained the eggs
may produce male prawns. These males fertilise the female‘s
eggs, resulting in a specific type of eggs. These are called
winter eggs and have a thick shell as protection. They’re pretty
rugged and can survive the swamp or lake drying out causing
the death of the entire prawn population for up to a decade in
a form of hibernation. The eggs hatch once the right ambient
conditions again obtain. The eggs supplied (Fig B 27) are of
this type.
11. Hatching of the salt water prawn
To hatch the prawn it is essential to first have a saline solution
suited to the prawn‘s needs. Fill half a litre of rain- or fresh-
water in a container. Let it stand for about thirty hours. As
water evaporates over time it‘s a good idea to have a second
container of such water left standing for thirty-six hours.
Once it‘s stood for this length of time pour half of the sea salt
supplied into one of the containers (Fig B 26) and stir until it has
dissolved. Then pour some of it into the prawn breeding plant
(Fig B 23). Add a few eggs and close the lid. Put it somewhere
with plenty of light but not in the direct sun. The temperature
should be approximately 25° C. The prawns will hatch in two
or three days at this temperature. Should any water evaporate
during this time replace it from the second container.
12. The saltwater prawn under the microscope
What comes out of the egg is known as a nauplius larva.
Use the pipette (Fig B 20) to put some of them on a slide for
examination. They will move in the solution using their hair like
limbs. Remove a few daily from the container for examination
under the microscope. If you do so and save the pictures made
with the MicrOcular you will then have a seamless record of the
prawn‘s lifecycle. You can remove the upper lid of the prawn
bleeding plant and put the whole thing under the microscope.
The larvae will mature in six to ten weeks depending on ambient
temperature. You will soon have bred an entire generation of
saltwater prawns that constantly reproduce.
13. Notes on cleaning
1. Avoid disassembling the microscope!
Because of being a precise optical instrument, do not
disassemble the microscope. That may cause serious
damage to its performance. Do not disassemble the
objectives.
2. Avoid contamination!
You should make sure that dust or moisture do not come
in contact with your microscope. Avoid putting fingerprints
on any optical surfaces! If dust or dirt nevertheless have
gotten onto your microscope, or the accessories, remove it
as described below.
3. Cleaning non-optical parts:
Disconnect the plug from mains socket before cleaning
non-optical parts of the microscope (e. g. the frame or the
stage). Firstly, remove dust or dirt from your microscope, or
accessories, using a soft brush. Then clean the soiled area
with a soft, lint-free cloth; the cloth may be moistened with
a mild detergent solution. Be sure that the microscope is
dry before using. For cleaning the painted parts or plastic
components, do NOT use organic solvents such as alcohol,
ether, acetone, xylene or other thinner etc.!
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