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Trouble Shooting BMS INVERTED BIOLOGICAL

Under certain condition, some no-fault factors will bring a reversible influence to the instrument’s

performance. If the problem is happened, please take

proper measures according to the follow table. If

you can’t solve the trouble by the supplied methods, please contact with the sales department of our

company.

PROBLEM REASON

SOLUTION

PAGE

I、Optical Part:

The plug of the lamp holder is
not connected into the
illumination set

Connect them well

The bulb burnt out

Change a new lamp

The brightness is too low

Adjust to a proper position

The color filter is piled too
much

Minimize the number of the
filters

1. The illumination
is opening, but the
field of view is dark.

No use the appointed lamp
bulb

use the specified halogen Lamp
6V30W

The nosepiece is not in the
located position

Turn the nosepiece into the
position where you can hear
“clicked”

the color filter is stopped
midway

Insert deeply

2. The edge of the
field of view has
shadow or the
brightness is
asymmetry

The phase contrast slider is not
located in the proper position

Turn the slider into the
“clicked” position

There are stains on the

specimen

Change a clean specimen

Find dust and

stain in the field
of view

There are stains and dust

on the eyepiece

Clean the eyepiece

4. appear double
image

the size of the aperture
diaphragm is too small

Open up the aperture
diaphragm

The nosepiece is not in the
center of the light path

ensure the nosepiece is turned
into the “clicked” position

the aperture diaphragm in the
view of field is opened too
large or too small

adjust the aperture diaphragm
correctly

The lens (condenser, objective,
ocular or culture dish)
become dirty

Clean all

In the phase contrast
observation, the bottom
thickness of the culture dish is
more than 1.2mm.

Use a the culture dish whose
bottom thickness is less than
1.2mm

5. Resolution
problems

：

Image is not
sharp

；

The contrast is
not high

；

The detail is
not clear

；

Don’t obtain
the phase
contrast effect

Use a bright field objective

Change to the phase contrast
objective

Содержание 74575

Страница 1: ...AL For safety and for exerting the best performance making you familiar with the instrument entirely it is strongly recommended that you read this manual carefully before using the microscope For the further reference please place this manual in a position where nearby the worktable and fetched easily ...

Страница 2: ...ntering the inside 6 When not using remember to cover up the microscope with the dust casing And make sure the 1 Do not keep the instrument in a direct sunlight high temperature or humidity dusty and easy shaking environment Make sure the stage is plane horizontal and stable enough 2 When moving the microscope please hold up the instrument with one hand on the lower side of the eyepiece tube and t...

Страница 3: ... Safety Symbol Symbol Meaning The surface is very hot not touch by your hands Before using please read the instruction carefully improper operation will result in bodily injure or instruction malfunction The main switch on The main switch off ...

Страница 4: ... slider Objective Lamp house Microscope base Stage fixed Objective turret Nosepiece fixed z Stage lengthen splint z Glass stage Quintuple Trinocular viewing tube fixed z 4X z 10X PH z 20X PH z 40X Ocular eyepiece Condenser Stage inserted plate Illumination bracket ...

Страница 5: ...tion steps Before installing be sure every components is clean do not score any parts or glass surface Keep well with the supplied hexagon wrench When changing the components you will need it again 2 Figure 2 Lamp house Microscope base Stage lengthen splint Mechanical ruler Power cord Objectiv Glass stage Ocular eyepiece Filter holder Condenser illumination unit Color filter ...

Страница 6: ...iphery are all cool Then you can do your replacing Please insert the lamp gently or it will be damaged by excessive extrusion Do not touch the Halogen bulb with your hands It will shorten the service life or cause it to burst If you leave fingerprints on the surface carelessly clean it with a dry soft cloth 2 2 2 Installing the condenser illumination unit figure 4 1 Insert the condenser illuminati...

Страница 7: ...nstalling the lamp house figure 5 Keep the BNC connector plugs and the lamp house pin in line and keep the bolt and the condenser jack in line too Then push the lamp house into the illumination unit gently until they are against ...

Страница 8: ...cording the magnification sequence of low to high Mount objective like this way will make the change of magnification to be very easy in using It also can install the objective through the stage opening Clean the objective regularly the objective used in the inversed microscope is very sensitivity about dust Do cover all the unused holes with turret dust caps to prevent the dust and contamination ...

Страница 9: ...echanical ruler together Generally the mechanical ruler will be installed in the right side for comfortable adjustment 1 Installing the stage lengthen splint First Screw the fixed bolt on to the splint then mount it on to the stage from right or left below screwing down it until it stay hard 2 Installing the mechanical ruler Please install the ruler like the way of the stage splint Figure 8 1 5 ...

Страница 10: ...t the eyepiece into tube until they are against 2 2 8 Installing the color filters fig 11 Be sure the color filter cools down completely before you change them Take down the filter holder then install the color filters you need Mount the color filter downwards like shown keep it horizontal through the end not allow inclined If the color filter is inclined or not get to the end it will drop possibl...

Страница 11: ...upply receptacle Make sure the connection is well 4 Insert BNC connector plugs into BNC connector jack Do use the supplied power cord all the time If lost or damaged select the same standard cord please Connect the power cord correctly to ensure the instrument is earthen 2 2 10 Replacing the fuse fig 12and 13 Do remember to turn the main switch on the state of O off before replacing the fuse and u...

Страница 12: ...GICAL 9 Figure 15 Diopter ring Light path selector lever Light switch Tension adjustment collar Main switch Coarse focus knob Fine focus knob Phase contrast slider Color filter holder Aperture diaphragm adjust lever Phase center bolt ...

Страница 13: ...rvice life 4 1 3 Adjusting the Tension Adjustment Collar fig 18 The tight tension of the coarse focus knob had already adjusted before leaving factory How to adjust the tight tension Turning the tension adjustment collar while revolving at the direction which shown by the arrowhead on the figure the tight tension of the coarse focus knob is increasing and if at the contra direction the tight tensi...

Страница 14: ... bottom thickness is 1 2mm and the same thickness is also required by the object slide when it is laid the specimen using the Φ35mm culture dish You can lay aΦ35mm culture dish on the stage directly by using the standard center board of the stage using the mechanical ruler 1 When using the 96bit or 24bit micro titration board please fasten it tightly by the stage clips 2 When fastening other model...

Страница 15: ...nt ring The number which the reticle on the eyepiece holder pointed is your eye s diopter graduation 4 3 2 Adjusting the interpupillar distance fig 22 When observing with two eyes hold on the left and right prism holder turn around the axis adjusting the interpupillar distance until the left and right fields of view coincide completely The reticle on the interpupillar distance indicator pointed by...

Страница 16: ... pulling out the lever until it reached the clicked position Light path selector lever Brightness proportion application Pushing in the lever until it reached the limit position 100 used for binocular observation Binocular observation Pulling out the lever until it reached the limit position 20 used for binocular observation and 80 used for video or photography Binocular observation television and...

Страница 17: ... LBD color filter which can compensate more neutral colors You could pile up a group of color filters to the filter holder if you ensure they are level and the whole thickness is less than 11mm Color filter meaning IF550 Single contrast color filter green used for the phase contrast microscopy LBD Color temperature transit color filter blue used for bright field observation and microphotography 14...

Страница 18: ... if necessary you also could insert in the center telescope then looked into the viewing tube you might see a field of view like the figure shown The proportion could be changed by dialing the aperture adjustment lever according your need is the image of the aperture diaphragm is the edge of the objective Generally when observing the chromatic specimen you need to set the size of the condenser ape...

Страница 19: ... objective please see 2 2 4 You ought to mount it on the turret 5 1 2 Phase contrast slider fig 27 phase centering adjustable slider The light ring was centered beforehand so it needn t to adjust in the use process If the ring is not in the center you could adjust by the centering bolt The 10X 20X light ring is worked with the 10X 20X phase contrast objective while the opening is used for bright f...

Страница 20: ...e light path 3 When in the phase contrast observation do keep the aperture diaphragm adjustment lever on the position of O wide opening 5 2 2 The centering ring fig 29 and fig 30 Usually you needn t the operation of centering If necessary please accord to the following steps 1 Place the specimen on the stage and focus it 2 Take out the eyepiece replace it with the CT the centering telescope and in...

Страница 21: ...ing center and the phase contrast center when changing the objective If having departure you ought to center again If the light ring is centering incorrectly you will fail to obtain the best viewing effect of the microscopy After removing or replacing a thick specimen the light ring and the phase contrast ring are likely to deviate each other which will result in a decline of the image contrast So...

Страница 22: ...he locking bolt on the trinocular viewing tube and take out the dust cap 2 Remove the dust cover on the both ends of the video accessories and revolve the screw head end into the CCD CMOS port 3 Install the accessories into the tri through port and screw down the bolt 6 1 3 Focus fig 32 Doing a binocular observation at 20 brightness look the image on the video or the computer which connected with ...

Страница 23: ... the digital photo connected head into the photo tube then screw down the locking bolts Before connecting the camera and adapter remove the camera lens firstly then connect the lens port with the adapter Pay attention to the gate type please To avoid the disturbing from the ocular in the observation please place the viewer finder on the two sides of the microscope when installing the camera set Th...

Страница 24: ...en Please refer the user manual of the camera set to obtain the details 6 2 4 Adjusting the color temperature When shooting the chromophotograph please use the sunlight film 1 Mount the LBD temperature changed color filter on to the color filter bracket 2 Turn the brightness adjustment knob to the maximal position so you obtain a sunlight illumination 20 ...

Страница 25: ...wn 3mm Stage Area 160 width 250 Length mm Mechanical ruler Movement Range 120 width 78 Length mm Illumination Halogen Lamp 6V30W Preset Center Intensity continued Adjustable Condenser Long working Distance Condenser Numerical Aperture 0 3 Working Distance 72mm Operation environment z Use indoor z Altitude Maximum 2000 m z Temperature 5 40 41 F 109 F z Maximum Relative Humidity 80 at 31 88 F then F...

Страница 26: ...t deeply 5 2 The edge of the field of view has shadow or the brightness is asymmetry The phase contrast slider is not located in the proper position Turn the slider into the clicked position 13 There are stains on the specimen Change a clean specimen 3 Find dust and stain in the field of view There are stains and dust on the eyepiece Clean the eyepiece 4 appear double image the size of the apertur...

Страница 27: ...dish the phase contrast ring and the light ring is deviated each other Moving the culture dish until you obtain the phase contrast effect You also could demount the slider and dail the field diaphragm with the direction of 13 The nosepiece is not in the center of the light path Insure the nosepiece is in the clicked position 4 The specimen don t place properly Place the specimen on the stage corre...

Страница 28: ... to spoil Change the bulb 3 2 The light glimpse The power cord have a poor contact Check the power cord and connect them exactly 6 Ⅳ Viewing tube The interpupillar distance is not correct Adjust the interpupillar distance 10 The diopter is not right Adjust the diopter 10 The two eyes field of view is different Not adapte to the microscope observation When look into the objective do not stare at th...

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