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23

Troubleshooting

Troubleshooting

Evaporation 

 

»

If the evaporation is uneven between wells: 

 

»

One or more nozzles may be clogged. Clean or replace 
the nozzle manifold, see page 11 and page 20.

 

»

One or more nozzles may be bent. Make sure the 
nozzles are aligned in straight rows. If the nozzles 
cannot be aligned, contact Biotage 

®

 1-Point Support 

.

 

»

The manifold and the well plate may be 
misaligned. To align the bottom heater to the 
manifold, contact Biotage 

®

 1-Point Support 

.

 

»

If the evaporation rate is too slow:

 

»

Check that the gas flow is not too low; see "Determine 
the Appropriate Gas Flow" on page 6.

 

»

Check there is no gap between the manifold and 
the gas heater. Check the screws are tightened.

 

»

Check that the gas inlet tube is not leaking. 

 

»

Check that the pressure regulator is set to the appropriate 
range. See the technical specification in TurboVap

®

 

96 Dual Installation and Safety, (P/N 418002).

 

»

If the evaporation rate is too rapid or splashing occurs:

 

»

Check that the gas flow is not too high, see 
"Determine the Appropriate Gas Flow" on page 
6 a
nd the technical specification in TurboVap

®

 

96 Dual Installation and Safety, (P/N 418002).

 

»

Check that the nozzles are not too close 
to the surface of the samples. 

 

»

If there is no gas flow:

 

»

Check that the gas supply is turned on and 
that the pressure is higher than the minimum 
pressure specified in TurboVap

®

 96 Dual 

Installation and Safety, (P/N 418002).

 

»

Check that the gas inlet tube is not 
obstructed or damaged.

 

»

If the recovery of sample is low:

 

»

Check that the plate heater temperature is not too high, 
thereby causing the solvent to boil; see "Determine the 
Appropriate Plate Heater Temperature" on page 7
.

 

»

Check that the sample is not left too long on the 
plate heater after the evaporation is completed.

 

»

Check that the gas flow is not too high and 
causing splashing; see "Determine the 
Appropriate Gas Flow" on page 6
.

 

»

Check that the gas heater temperature is not too high, 
thereby causing the solvent to boil; see "Determine the 
Appropriate Gas Heater Temperature" on page 7.

Gas Supply

 

»

If a gas leak has been detected at the gas inlet (the 

N2

 port):

 

»

Check that the gas inlet tube is securely attached.

 

»

Check that a gas inlet tube with the correct 
outer diameter is used, i.e. 6 mm. Always use 
tubing and adapters supplied by Biotage.

 

»

If the instrument warns for too low gas pressure or the 
flow rate does not reach the expected set value:

 

»

Check that the pressure at the inlet (the N2 port) 
meets the minimum pressure requirement specified in 
TurboVap

®

 96 Dual Installation and Safety, (P/N 418002). 

 

»

Check that the provided inlet tube is used. A 
longer tube may cause pressure drop.

Touch Screen

 

»

If the touch screen is not responding as 
expected, test its calibration:

 

»

Press 

More

 in the settings view and then 

Verify Screen

.

 

»

Draw or write on the touch screen to 
verify its function and calibration.

 

»

If the touch screen calibration is off, calibrate it:

 

»

Press 

More

 in the settings view and 

then 

Calibrate Screen

 

»

Follow the instructions on the screen and then test 
the calibration as described in the bullet above.

 

»

If the touch screen calibration wizard cannot be 
accessed due to a faulty calibration, restart the system 
and repeatedly open and close one of the doors during 
start-up. This will open the calibration wizard. 

Note:

 The door only needs to be opened approximately 20 

mm.

Lift

 

»

If there is an error message regarding a lift:

 

»

Restart the system. Should the error persist, 
contact Biotage 

®

 1-PointSupport 

.

Содержание TurboVap 96 Dual

Страница 1: ...Patent Pending Biotage 2021 Title Truncated if Neccessary Page 1 TurboVap 96 Dual User Manual...

Страница 2: ...8 Define System Settings 8 Common Settings 9 Evaporation Settings 10 Network and Firmware 10 System Information 10 Warnings in the Software 11 Prepare the System 11 Replace the Manifold 11 Set the Pr...

Страница 3: ...s manifolds with 24 48 or 96 nozzles to allow for processing of up to 96 samples in each cabinet The left and right cabinet can perform simultaneous evaporation runs with varying size well plates vary...

Страница 4: ...he door is closed the evaporation run will resume To determine the correct plate height for an evaporation run see Determine Optimal Evaporation Conditions on page 6 Evaporation To provide consistency...

Страница 5: ...the Manifold on page 11 according to the chosen well plate Available manifolds TurboVap manifold with 24 gas nozzles TurboVap manifold with 48 gas nozzles TurboVap manifold with 96 gas nozzles Differe...

Страница 6: ...radation of analytes may be observed Do not allow samples or solvents to boil The evaporation time of samples decreases with increasing sample cleanliness The presence of sample detritus or particulat...

Страница 7: ...plate Usually setting the temperature of the plate heater 0 20 C above the gas temperature allows for enhanced evaporation uniformity Use of the plate heater minimizes cross contamination of samples w...

Страница 8: ...ifying and calibrating the touch screen 1 Mode Mode selection Press to select mode 2 Common Settings Press to change common settings 3 Evaporation Settings Press to change evaporation settings 4 Netwo...

Страница 9: ...as temperature Tick the box to wait for the gas temperature to reach the set value before starting an evaporation Wait for plate temperature Tick the box to wait for the plate temperature to reach the...

Страница 10: ...ress the Reset Settings button to reset all settings in Common settings Evaporation Settings and Network and Firmware to their default values Calibrate Touch Press the Calibrate Touch button to calibr...

Страница 11: ...ure 17 Ensure that the manifold is held in position while the last screw is removed 3 With a steady grip remove the manifold Note Do not hold the manifold by the nozzles as this might bend the nozzles...

Страница 12: ...Time Mode Evaporate for a set period of time 4 Method Mode Evaporate using a predefined method 5 Split to left and right OR Join left and right The left and right cabinets operate independently OR The...

Страница 13: ...splay shows that the left side is ready to start the orange Start button is active and the right side is running an evaporation the blue Pause button and the red Stop button are active To perform a Ti...

Страница 14: ...ode In the Join left and right mode the left and right cabinets operate the same evaporation run with the same parameters see Figure 23 Figure 23 Temp Step Method 96 run in Join left and right mode Du...

Страница 15: ...utton to enter the desired name see Evaporation Parameters on page 15 6 When done press Save Note Once a method has been saved its previous settings cannot be recovered 7 In the Methods view press OK...

Страница 16: ...ers view where the evaporation parameters or the method name can be edited or a method can be copied Figure 29 Press and hold the Plate height button then move the lift by pressing the Up and Down arr...

Страница 17: ...an alarm sounds If operating each cabinet separately then the LED lights in each cabinet will turn green independently The Evaporation Finished dialog appears on the screen see Figure 34 The alarm co...

Страница 18: ...l risk for burn damage Open the cabinet door The well plate will descend Carefully remove the well plate or rack Be careful not to come in contact with the nozzles See Figure 35 Note The lift will ret...

Страница 19: ...ly dampened with isopropanol or ethanol 3 When the system has been cleaned connect the power cord and turn on the system Clean the Interior of the System Warning Never clean the surface where the mani...

Страница 20: ...following is recommended a For polar analytes chose solvent combinations with a high aqueous content e g an 80 20 solution of water and methanol b For lipophilic analytes chose solvent combinations wi...

Страница 21: ...plied by Biotage Incorrect fuses will void the warranty of the system and creates a potential fire hazard and a risk of personal injury 1 Turn off the system and disconnect the power cord 2 Using a fl...

Страница 22: ...into Storage Warning Ensure not to bend any of the nozzles If a nozzle is bent it is recommended that the manifold is replaced 1 Remove and clean the manifolds as described on page 20 2 Place the man...

Страница 23: ...er Temperature on page 7 Check that the sample is not left too long on the plate heater after the evaporation is completed Check that the gas flow is not too high and causing splashing see Determine t...

Страница 24: ...crew 1 Consumables Part No Description Qty 121 5201 Collection Plate 350 uL Square 50 121 5202 Collection Plate 1 mL Square 50 121 5203 Collection Plate 2 mL Square 50 121 5213 Collection Plate 2 mL R...

Страница 25: ...25 Notes Notes...

Страница 26: ...art Number 418001 B 2021 Biotage All rights reserved No material may be reproduced or published without the written permission of Biotage Information in this document is subject to change without noti...

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