20
TurboVap
®
96 Dual User Manual | © Biotage 2021
Maintenance
Clean the Manifolds
Warning
»
Ensure not to bend any of the nozzles. If a nozzle is bent, it is
recommended that the manifold is replaced.
»
The nozzles may have come into contact with hazardous
samples. Avoid contact.
Clean the manifolds regularly. The frequency of cleaning
should be determined by the needs of the laboratory, but it is
recommended that the manifolds should be cleaned when:
»
a manifold may have been contaminated,
»
a manifold has been in contact with
harsh solvents or additives,
»
a nozzle is obstructed,
»
before long term storage.
The most thorough way to clean the needle assemblies is to
remove the manifolds and clean them outside of the instrument.
However, in-situ rinsing can be performed as a quick
alternative.
The nozzles are made of 316 stainless steel (EN 1.4404), and the
head assembly is made of anodized aluminum (aluminum, EN
AW-6082). Always consider the material of the instrument parts
when selecting cleaning agent.
Clean a Manifold in Place
This method requires a suitable container that can be filled with
cleaning agent and that fits on the plate heater. A well collection
plate (2 mL or larger) is recommended, see "Consumables" on
page 24.
Note:
Ensure that the well collection plate fits the manifold, i.e.
for a 24-nozzle manifold use a 24-well collection plate.
1. Fill the container with an appropriate cleaning solvent. The
solvent is dictated by analyte solubility and the following is
recommended:
a. For polar analytes chose solvent combinations with a
high aqueous content, e.g., an 80/20 solution of water
and methanol.
b. For lipophilic analytes chose solvent combinations with
a high organic solvent content, e.g., an 80/20 solution of
methanol and water.
2. Place the container on the plate heater under the manifold.
3. Close the door and lift the container so that the nozzles are
submerged in the desired cleaning solvent. (Do not start an
evaporation run.)
4. Keep the nozzles submerged in the solvent for 5-10 minutes.
Note:
For polar analytes it is recommended to repeat step 1 to 4
with a high organic solvent combination after the high aq rinse.
5. Lower the container and remove it.
6. Ensure that the nozzles are dry before initiating an
evaporation run. Either wait until they are dry, or perform an
evaporation run with an empty cabinet and unheated gas.
(25 °C/77 °F)
Clean a Manifold Outside of the Instrument
This method requires the manifold to be removed from the
instrument and placed in a sonicator. Additionally, the method
requires a suitable container that can be filled with solvent.
A well collection plate (2 mL or larger) is recommended, see
"Consumables" on page 24 .
1. Remove the manifold as described in "Replace the Manifold"
2. Prepare a container as in "Clean a Manifold in Place" on
3. Place the container in a sonicator. Ensure that the water level
is below the edge of the container. Water in the container
will lead to contamination.
4. Carefully lower the manifold into the container and sonicate
for 5-10 minutes.
a. If contamination is present, the times and solvent
variants may need to be adjusted.
