411829-L, Operation
April 2012
Page 4-22
4
Replace and Allocate Racks During the Run
If more fractions are to be collected than can fit in the allocated rack(s), the system pauses, the
collection arm returns to the home position (the inner right corner), and the
Load New Racks
dialog opens. Replace the rack(s) according to the dialog and press the
Resume
button to resume
the run. The collection is resumed in the first vessel in the lowest lettered rack. For example, if
racks B and C are allocated for the run, the collection is resumed in rack B in vessel 1.
You can at any time allocate free rack positions by pressing
Edit...
at the
Status
tab. (This is not
possible if automatic rack allocation is enabled.)
NOTE
When using an Isolera Four system with automatic rack allocation enabled,
you have to replace all racks in the collection area at the same time when
asked to (even if some of the racks have been used by previous runs).
Auto-Extend Mode
If the system is collecting fractions and remaining light absorption is detected when the system
nears the end of a purification, the system enters the Auto-Extend mode (if enabled in the system
mode). This extends the gradient purification stage of the run with 25% of the total gradient length
using the final conditions in the method.
Line Flush, Decompression, and Detector Flush
After the gradient purification stage is finished, the system performs a line flush (if enabled), air
flush (if enabled), system decompression, and a detector flush (if enabled). To enable or disable
flushes, specify whether enabled flushes are collected or not, and specify which solvents to use for
the flushes, see “Specify How Flushes Are Performed” on page 3-10. Note that the Air Flush feature
is not available with Isolera Prime.
4.6 Change the Processing Order
When using an Isolera Four system, it is possible to change the processing order. To move up a
purification in the queue, press the button at the
Status
NOTE
Queued equilibrations take priority over queued gradient runs.
Figure 4-17. Change the Processing Order
If you press this
button, the run
on cartridge 3
will be performed
prior to the run
on cartridge 1
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