Biotage Isolera Four, Руководство пользователя

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411829-L, System Settings April 2012 Page 3-10
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6. In the Chemistry mode, enter the capacities and current fluid levels for the solvent and/or
waste reservoirs; see “Set the Reservoir Volumes” on page 4-32. (For the system to be able to
maintain a running calculation of fluid levels in each reservoir, the capacities and current fluid
levels for the reservoirs must be entered each time you empty a waste reservoir or replenish a
solvent.)
To disable solvent and/or waste monitoring:
1. Select the Runtime tab in the right-hand panel.
2. Select the Solvent Monitoring and/or Waste Monitoring text box and select No .
3.6.6 Enable or Disable Run Requirement Estimation
If you enable vessel, solvent, and waste estimation, you will be informed (when allocating the
cartridge and rack positions) if a sufficient quantity of vessels is allocated, if a sufficient quantity of
the correct solvent is present in each solvent reservoir, and if the waste reservoir has sufficient
capacity for the run.
To enable or disable vessel, solvent, and/or waste estimation, select the corresponding text box and
select
Yes or No . If using an Isolera Four system, ensure to select the number of waste reservoirs
to be used in the Waste Reservoir text box. The waste can either be pooled into a single waste
reservoir (insert all four waste outlet tubes into a single waste reservoir) or collected in four waste
reservoirs (insert each waste outlet tube into a single waste reservoir).
3.6.7 Specify How Flushes Are Performed
At the end of a purification, i.e. after the gradient purification stage is completed, the system
performs a:
• Line Flush : The system flushes the inlet line and the cartridge with 9 ml (when using an
Isolera Prime, Isolera One, or Isolera Four system) or 20 ml (when using an Isolera LS
system) of the specified solvent or solvent mix.
• Air Flush: The system flushes the inlet line and the cartridge with air and a small amount of
the specified solvent or solvent mix. The flush volume depends on the cartridge type.
Air Flush is optional. This feature is not available with Isolera Prime.
• Detector Flush : The system flushes the detector with 27 ml (when using an Isolera Prime,
Isolera One, or Isolera Four system) or 45 ml (when using an Isolera LS system) of the
specified solvent or solvent mix.
To change the flush settings:
1. Select the Runtime tab in the right-hand panel.
2. To enable or disable the line flush, select the Line Flush text box and select Collect (the line
flush will be collected on the collection tray), To Waste (the line flush will be sent to waste), or
Off (no line flush will be performed).
3. To enable or disable the detector flush, select the Detector Flush text box and select Collect
(the detector flush will be collected on the collection tray), To Waste (the detector flush will be
sent to waste), or Off (no detector flush will be performed).
NOTE
If the automatic line flush is disabled, ensure to prime the cartridge flow
path before each run.
We recommend that the detector flush is kept enabled. A dirty detector
flow cell has decreased transmissivity, which causes increased noise level,
decreased response, and difficulties performing UV Zero.