9
6.
Interpretation of the
Measurement Results
6.1.
Accuracy.
Accuracy is determined for selected wavelengths at six absorbance
levels, each of which covers eight well positions in a row. Each of the
20 measurements of the OD plate is compared to the accuracy crite-
ria as follows:
(Test value
– Reference absorbance value) × 100 < Accuracy criteria.
-
The accuracy test will be passed only if all test values meets the
specifications
Accuracy
Criteria
0
– 2.3 OD ≤ (0.5 % ± 0.010 OD) typical
2.3
– 3 OD ≤ (1 % ± 0.010 OD) typical
3
– 4.3 OD ≤ (2 % ± 0.010 OD) typical
4.3
– 5 OD ≤ (15 % ± 0.010 OD) typical
6.2.
Precision
Precision here is the coefficient of variation (CV%), which is deter-
mined for any row after reading the OD Plate 20 times. The CV% is
calculated as follows:
𝐂 =
𝑺
𝑴
× 𝟏𝟎𝟎%
Where
C
is CV%,
S
is Standard deviation between and
M
is Mean.
The precision test will be passed only if the average precision at each
absorbance level and at each channel meets the specifications.
Precision Criteria
0
– 2.3 OD ≤ 0.5 %
2.3
– 3 OD ≤ 1.0 %
3
– 5 OD ≤ 2.0 %
