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OPERATION NOTES
Your microscope has a factory built-in
multi-mode universal turret condenser.
By simply rotating the condenser turret
left or right, you can change the
condenser mode from brightfield to
darkfield to phase contrast and even to
a 3D image perspective.
Condenser Modes
DF—
PH3—
PH2—
PH1—
BF—
2X—
FL —
Darkfield
Phase Contrast using 60x and 100x oil phase objectives.
Phase Contrast using a 40x phase objective
Phase Contrast using a 10x or 20x phase objective
Brightfield (note that this setting has an associated condenser
iris adjuster and set-screw to set image contrast/depth of field).
Generally for live blood viewing you would close the iris quite a
bit to give you more contrast and depth of field, and open it up
for a dry blood view to give you less contrast and depth of field
This is when using the 2X plan objective so you can see a wider
field of view when looking into the eyepieces.
This is for reflected light fluorescence viewing (Scope as shipped
is not equipped for fluorescence.)
The most popular Biomedx configuration is set up for non-oil microscopy using all
condenser modes and will have 2x, 4x, 10x, 20x, 40x objectives. The 2, 4 & 10x optics
are typically used for brightfield viewing (the 10x will give a very wide field darkfield
view) The 20x is a phase objective and you will find PH1 indicated on the lens barrel
telling you to use the PH1 setting on the turret. You can also use DF. The 40x is a
phase contrast objective and an everyday working optic for live cell work. It uses the
PH2 turret setting for phase contrast, DF will provide darkfield, and if you slightly shift
the turret out of the DF indent position, you will have a 3D view of your sample.
