Chapter 7 Creating Experiments and Workspaces
Note:
In high-throughput Acquisition mode, the Pause After, Volumetric Counting, and Return
Sample functions are disabled. High-throughput mode does not support event limits or gate limits,
only volume limits can be used. All samples in a high-throughput run must use the same target
flow rate.
Pausing after a Tube or Well
Note:
Pause After is disabled when high-throughput mode is activated.
Pause After can be useful when applying automatic compensation. Activate Pause After on the last
compensation control position in the experiment. Acquisition automatically stops after the file for that
control is recorded. You can then navigate to the Analysis module to perform automatic compensation
and then return to the experiment to acquire and record sample files along with the compensation
matrix, which are exported in the FCS file for analysis in third-party software.
To pause after a tube or well
1.
In the plate map, select the relevant positions.
2.
Click the Pause After button.
The run list pauses after the applicable position has been acquired. Positions for which pause
after is activated are marked with an image in the plate map. For more information, see
.
Setting Volumetric Counting on Samples
To verify the number of cells in a specified volume of each sample, activate Volumetric Counting.
Activating Volumetric Counting automatically activates Return Sample. Excess sample in the line
returns to the tube or well after target volume is acquired.
Note:
You can set Return Sample to OFF if necessary. However, when using Volumetric Counting
with Return Sample set to OFF, you might experience sample loss. If sample loss is a concern,
run Volumetric Counting with Return Sample set to ON.
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Страница 50: ...Chapter 2 Hardware Description 48 ZE5 Cell Analyzer and Everest Software ...
Страница 82: ...Chapter 4 Everest Software 80 ZE5 Cell Analyzer and Everest Software ...
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Страница 109: ...Managing Users 9 Click OK to save the new user account User Guide 107 ...
Страница 120: ...Chapter 5 Configuring the System 118 ZE5 Cell Analyzer and Everest Software ...
Страница 125: ...Working with Optical Filter Configurations User Guide 123 ...
Страница 126: ...Chapter 5 Configuring the System 124 ZE5 Cell Analyzer and Everest Software ...
Страница 173: ...Setting Up the Run List The plate setup displays a layout of the selected media User Guide 171 ...
Страница 177: ...Setting Up the Run List User Guide 175 ...
Страница 191: ...Setting Up the Run List User Guide 189 ...
Страница 276: ...Chapter 7 Creating Experiments and Workspaces 274 ZE5 Cell Analyzer and Everest Software ...
Страница 295: ...Setting Up the Experiment 6 Set up samples User Guide 293 ...
Страница 333: ...Decontaminating the System User Guide 331 ...
Страница 380: ...Appendix B Example 9 Color Immunophenotyping Experiment 378 ZE5 Cell Analyzer and Everest Software ...
Страница 384: ...Appendix C Optional External DI Water and Waste Ports 382 ZE5 Cell Analyzer and Everest Software ...
Страница 392: ...Appendix F References 390 ZE5 Cell Analyzer and Everest Software ...
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