•
Each time you place a new blank into SmartSpec 3000, press Read Blank. After
the absorbance reading is collected, the instrument is zeroed and the display will
show
You can check the absorbance reading at any time by pressing Abs.
•
Press the right arrow key to return to the assay.
After all the blanks are read, SmartSpec 3000 will prompt you for the number of
standards, the units of concentration for those standards, and then the number of
standard replicates. If you do have replicates, then it will ask you if you have the same
number of replicates for each standard. SmartSpec 3000 allows you to have differing
numbers of replicates for each standard.
•
Use the number keys to input the number of standards and press Enter. Next
SmartSpec 3000 will prompt for the units of concentration. Your standards must all
have the same units.
•
Press Select until you see the desired units of concentration and then press Enter. As
you press Select, other choices of units are displayed, each new choice representing
a reduction of one order of magnitude in concentration, or you can choose to have no
units.
Next SmartSpec 3000 will ask if any of the standards are to be read in replicate.
•
If you have prepared any of your standards in replicate, press Select to toggle from
No to Yes and press Enter. Skip ahead to ii. If none of the standards are prepared
in replicate, press Enter.
31
Blanking
Spectrophotometer
A750 = 0.000
>=Continue
Enter the number of
standards (2–9):
Select concentration
units: µg/ml
Select concentration
units: mg/ml
Select concentration
units: µg/ml
Select concentration
units: ng/ml
Select concentration
units: none
