BIO RAD SmartSpec 3000, Инструкция по эксплуатации

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Section 5
Keypad Description
Print SmartSpec 3000 will print data on the current sample, a
user-selectable range of samples or all samples in the
current assay.
Abs SmartSpec 3000 will automatically display the
absorbance reading of the current sample and the
wavelength being read. If more than one wavelength is
being read, press Abs to toggle among the different
wavelengths. If you are reading one of a replicate group,
you can choose to see the absorbance of the current
replicate or you can see the mean and standard
deviation of all members of the replicate group by first
pressing Abs.
Conc The Conc key is used in the DNA Oligo and RNA Oligo
assays to toggle the display of concentration from units
of µg/ml to pmoles/µl. This key has no function for the
Scan, Kinetics and
λ
assays.
A260:A280 SmartSpec 3000 will calculate and display the ratio of
A260 to A280. This is a method of approximating the
purity of nucleic acid preparations; pure preparations of
DNA have ratios of about 1.8 and pure RNA preparations
have a ratio of about 2.0. If you are using background
compensation (in Setup), then the ratio is calculated after
the background reading of A320 is subtracted from the
A260 and A280.
Dilution Factor The dilution factor is used as a final multiplier in calcu-
lation of concentration from absorbance data. The
default dilution factor is 1.0 and the dilution factor is
reset to 1.0 each time that one of the Assay buttons is
pressed.
Setup SmartSpec 3000 will enter the setup routine where you
can specify the pathlength, defaults for certain options,
time and date convention and time and date. You exit
Setup either by proceeding all the way through the rou-
tine, or by pressing Cancel at any time. When you exit
the Setup routine, all changes are saved.
Assay Buttons The six assay buttons are listed below. Press one of these
to begin an assay with SmartSpec 3000. When any assay
button is pushed, SmartSpec 3000 resets the dilution fac-
tor to 1.0, the sample number to 1, and checks the data
buffer for unprinted data.
DNA/RNA SmartSpec 3000 will automatically collect absorbance
data at 260, 280 and 320 nm, and use those readings to
calculate nucleic acid concentration. The calculation of
concentration depends on the type of nucleic acid
specified.
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