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QX200 Droplet Reader and

QuantaSoft Software

Instruction Manual

Catalog # : 1864001

1864003

Содержание QX200

Страница 1: ...QX200 Droplet Reader and QuantaSoft Software Instruction Manual Catalog 1864001 1864003...

Страница 2: ......

Страница 3: ...ive owner EvaGreen is a trademark of Biotium Inc Bio Rad Laboratories Inc is licensed by Biotium Inc to sell reagents containing EvaGreen dye for use in real time PCR for research purposes only The QX...

Страница 4: ...ruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required...

Страница 5: ...in the Code of Federal Regulations CFR at 29 CFR 1910 132 General requirements 29 CFR 1910 138 Hand protection 29 CFR 1926 95 Criteria for standard personal protective equipment Any gloves with impai...

Страница 6: ...iv QX200 Droplet Reader and QuantaSoft Software Instruction Manual...

Страница 7: ...2 Using the Experiment Editor 12 3 1 3 Using the Advanced Options 13 3 2 Run 13 3 3 Analyze 14 3 3 1 Viewing Channel Data 16 3 3 2 Viewing Scatter Plots 18 3 3 3 Viewing Concentration Data 19 3 3 4 V...

Страница 8: ...vi QX200 Droplet Reader and QuantaSoft Software Instruction Manual vi QX200 Droplet Reader and QuantaSoft Software Instruction Manual...

Страница 9: ...following PCR for downstream applications such as sequencing or cloning The ddPCR System lets you Detect rare DNA target copies with unmatched sensitivity Determine copy number variation with unrivale...

Страница 10: ...be different Component Description Catalog QX200 Droplet Generator QX200 Droplet Generator Instrument used for droplet generation 1864002 DG8 Droplet Generator Microfluidic cartridge used to mix sampl...

Страница 11: ...rol for Probes 1863052 Reagents for EvaGreen detection PCR supermix QX200 ddPCR EvaGreen Supermix 1864033 1864034 1864035 1864036 Droplet generation oil Droplet Generation Oil for EvaGreen 1864005 186...

Страница 12: ...ad after thermal cycling and four will not be read Analyze results the droplet reader connects to a laptop computer running QuantaSoft Software The software provides a complete set of tools for settin...

Страница 13: ...he lights are flashing amber the run cannot be started replace the oil or waste bottle see Section 4 2 2 Table 2 1 Status indicator lights on the QX200 Droplet Reader Solid green Power on Bottle level...

Страница 14: ...lid again to close the cover Confirm the first three indicator lights are green Table 2 1 Fig 2 Placing the 96 well plate into the plate holder Base of plate holder 96 well PCR plate Top of plate hol...

Страница 15: ...te holder lid is flat on top of the plate and the clamps are locked down Failure to do so could cause the sampling needle to run into the lid damaging the instrument Fig 4 Correct and incorrect placem...

Страница 16: ...8 QX200 Droplet Reader and QuantaSoft Software Instruction Manual 8 QX200 Droplet Reader and QuantaSoft Software Instruction Manual...

Страница 17: ...see Section 3 1 Run start the run and control the instrument if needed see Section 3 2 Analyze compute nucleic acid concentration see Section 3 3 QuantaSoft Software uses the following file types Tem...

Страница 18: ...te click Template New To overwrite the setup information for a plate that is open experiment type and name sample name etc click Template Load In the Load Template window click Overwrite Fig 5 QuantaS...

Страница 19: ...In the Sample panel enter the sample Name and select the Experiment from the dropdown menu All saved experiments appear in the dropdown menu along with the option to add experiment The sample name is...

Страница 20: ...n the experiment editor select Experiment add experiment in the well editor or select New or Edit double click on an experiment name in the Experiments window under Setup Three types of experiments ar...

Страница 21: ...ross the top navigation area Once the run is complete all data are reanalyzed for the final data file 3 2 Run 1 Click Run in the left navigation bar to start the run 2 In the Run Options window select...

Страница 22: ...election of wells for targeted analysis Processed data graphical display allows visualization of graphical data from selected wells Fig 13 Interface during an active run Data for both detector channel...

Страница 23: ...ual analysis tools To get more details of an error hover over the check tab with the mouse arrow Multi data automatically analyzed as part of a multi well selection Manual droplets analyzed manually F...

Страница 24: ...processed data plots show where droplet data from each well start and end and the assigned threshold appears as a horizontal pink line Use the multi well threshold tool to change the threshold in all...

Страница 25: ...nt tool single well Reset automatic threshold settings Y axis log scale toggle Threshold settings Fig 18 Viewing channel data for multiple wells Processed data from both channels of multiple wells are...

Страница 26: ...he whole plot the clustering tool is disabled when viewing the plot in heat map mode Use the ellipse rectangle or lasso threshold adjustment tool to classify a region of the plot Click the tool then c...

Страница 27: ...oisson confidence limits Solid data points shown indicate merged data open data points not shown indicate data from single wells Toggle y axis log scale Display data from single wells merged data or b...

Страница 28: ...reaction Chapter 3 Using QuantaSoft Software Toggle well name or label display Fig 21 Viewing copy number data Hover over data points to reveal well identity concentration and Poisson confidence limi...

Страница 29: ...the desktop Open this mode from the Start menu otherwise files automatically open in the regular version of QuantaSoft Simulation mode allows you to perform functions from the top right menu that are...

Страница 30: ...22 QX200 Droplet Reader and QuantaSoft Software Instruction Manual 22 QX200 Droplet Reader and QuantaSoft Software Instruction Manual...

Страница 31: ...ree 2 indoor use Installation category II external power supply plugs into standard AC receptacle Ventilation requirements 5 12 cm left and right of machine and 10 25 cm behind should be unobstructed...

Страница 32: ...ader fluids are based on fluorinated hydrocarbon chemistry and should be disposed of in accordance with institutional state and local regulations These nonflammable fluids are inert and have low envir...

Страница 33: ...roducts which will serve as a substrate for microbial growth mold and fungus To avoid instrument damage always be sure to add 50 ml of 10 bleach to the empty waste bottle to prevent microbial growth F...

Страница 34: ...ice You should contact Technical Support All other error codes Since the computer directly controls the instrument s fluidics problems with computer performance can trigger instrument errors The first...

Страница 35: ...for use in nucleic acid sample preparation with the QX200 Droplet Generator 1863024 ddPCR Supermix for Probes No dUTP 5 ml 5 x 1 ml 2x supermix for use in nucleic acid sample preparation with the QX2...

Страница 36: ...leic acid sample preparation with the QX200 Droplet Generator 1864035 QX200 ddPCR EvaGreen Supermix 25 ml 5 x 5 ml for use in nucleic acid sample preparation with the QX200 Droplet Generator 1864036 Q...

Страница 37: ...Pkg 1 000 rxns 12003805 ddPCR HDR Ref Assay Predesigned 100 rxns 12003806 ddPCR HDR Ref Assay Predesigned 500 rxns 12003793 ddPCR HDR Ref Pkg Predesigned 1 000 rxns 12002314 ddPCR NHEJ Gene Edit Assa...

Страница 38: ...30 QX200 Droplet Reader and QuantaSoft Software Instruction Manual...

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Страница 40: ...ungary 36 01 459 6190 India 91 124 4029300 Israel 972 03 963 6050 Italy 39 02 49486600 Japan 81 3 6361 7000 Korea 82 2 3473 4460 Mexico 52 555 488 7670 The Netherlands 310 318 540 666 New Zealand 64 9...

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