24 | QX200 Droplet Reader and QuantaSoft Software Instruction Manual
24 | QX200 Droplet Reader and QuantaSoft Software Instruction Manual
Chapter 4 Specifications and Maintenance
4.2 Maintenance
4.2.1 General Maintenance Procedures
Surfaces of the instrument may require general cleaning. Use deionized/distilled water for general wipe down
with a slightly dampened cloth. For decontamination, 10% bleach followed by 70% ethanol and/or deionized/
distilled water may be used. Do not use acetone or tap water.
Inspect equipment regularly for damaged external components or wiring. Do not use if damaged.
Apply standard MSDS (Material Safety Data Sheet) and OSHA practices when handling and disposing of
generated waste. When disposing of the reader oil waste, consult with your local environmental health and
safety specialists for guidance appropriate to disposal at your locality. You can find more MSDS available
online. A typical waste profile should contain the following: fluorinated oils (95%), water (5%), bleach (<0.5%),
proteins, nucleic acids, fluorescent dye (<0.1%).
Bio-Rad droplet generation and reader fluids are based on fluorinated hydrocarbon chemistry and should be
disposed of in accordance with institutional, state, and local regulations. These nonflammable fluids are inert
and have low environmental impact and low toxicity. Collect waste in a polyethylene container and discard
within one month.
Droplets made with Bio-Rad supermix have antimicrobial properties, but microbial growth is possible.
The waste profile should contain the following: fluorinated hydrocarbons, water, fluorescent dye (from probes),
protein, and nucleic acids.
Do not replace detachable power cord with an uncertified or an inadequately rated cord.
Since the QX200 Reader contains moving parts, fluids, tubing, and pumps, we strongly recommend annual
preventive maintenance on the instrument.
4.2.2 Replacing Droplet Reader Oil and Removing Waste
Replace the droplet reader oil and waste receptacle as needed. Use the handle built into the side
compartment to slide the carriage out:
■
■
Use each empty oil supply bottle as a new waste bottle only once, then discard. Add 50 ml 10% bleach to
the waste bottle to prevent microbial growth and place a label on the waste bottle to identify it as waste
Discard the waste bottle after one time use. Reuse may lead to microbial growth, instrument
damage, or run failures.
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