Fig. 5.2. Attachment of tubing fittings.
3.
Insert one end of the tubing into the tubing retaining bracket of the pump
head. Lightly pull the tubing around the rollers to remove slack. Attach
the other end of tubing into the tubing retaining bracket on the opposite
side of the pump head.
4.
Slide the platen back into the pump head frame assembly until it rests up
against the tubing. Press the cam lever in toward the pump head, lock-
i ng the platen up against the tubing and rollers. Note that the platen can
be inserted with the cam lever on the left or the right.
5.
Proper adjustment of platen pressure increases flow stability, minimizes
flow pulsation, and prolongs the life of the tubing.
6.
Attach system tubing to the pump tubing using Bio-Rad's luer-type fit-
tings or other suitable connectors. Lock-rings are not necessary for sys-
tem tubing.
5.3 Installation of Uncut Tubing
Tubing length and the amount of tubing prestretch have a significant
effect on both flow rate calibration and reproducibility of flow. The PharMed
tubing supplied with the Model EP-1 Econo Pump has been cut to a prede
termined length to accommodate tubing prestretch. When using any tubing
that is not properly sized, the tubing must be cut to accommodate prestretch.
Tubing should be cut as follows:
Note: Overtightening the platen adjustment screw will reduce flow rate
and shorten tubing life. If the platen is too loose, flow rate will decrease
as backpressure increases.
Tubing
Length
Tygon, PharMed
1 79 mm +/- 1.5 (7.04" +/- 0.05)
Silicone
171 mm +/- 1.5 (6.75" +/- 0.05)
Install tubing onto the pump head as described in Section 5.2.
Warning: If using tubing other than the type supplied by Bio-Rad, make
sure the wall thickness is not greater than 1.0 mm. Using tubing with a
greater wall thickness can damage the pump and void your warranty.
a.
Loosen the platen adjustment screw, located on the front of the plat-
en, counterclockwise until the stop is reached.
Section 6
Operation
b. Cut tubing to proper length and install fittings and lock-rings as
described in Section 5.2. Install tubing in pump as described in Figure
5.l. Start the pump and set flow rate to the value you expect to use for
your application.
c. Turn adjustment screw clockwise just until uniform flow is achieved.
This will be evident by observing the uniform motion of bubbles in the
line (if any) or a motion-free inlet line when operating with the expect-
ed backpressure.
Typically, one piece of silicone tubing will perform well with two turns
clockwise from stop. One piece of Tygon or PharMed tubing will perform
well between three and four turns clockwise from stop. When using two
pieces of tubing, approximately 50% more turn clockwise from stop will
be required. A clockwise stop will occur at eight turns clockwise.
6.1 Start-Up Procedure
1.
Plug the Model EP-1 Econo Pump into an appropriate
grounded power source. Turn on the power switch on
the bottom of the unit. The pump will power up in the
Stop mode, with the LED display showing 0% of maxi
mum speed.
2.
With tubing in place, set the desired pump speed by
pressing the Arrow keys.
3.
Press the Run/Stop key to initiate flow. Press once more
to stop the flow. Flow rate will be displayed in percent
of maximum speed. Change the flow to the desired
speed while the pump is running by pressing the Arrow
1 0
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