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BIO RAD Bio-Plex Pro, Инструкция по эксплуатации

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Basic Operation and Maintenance 

Bio-Plex Pro and Bio-Plex Pro II Wash Stations                      

                 11 

Basic Operation and Maintenance 

This section gives you instructions to configure, install, prime, and use the Bio-Plex 
Pro™ and Bio-Plex Pro II  Wash Stations. You can perform your first plate wash 
simply by using these instructions and the onboard wash program. This section also 
contains references to more detailed information throughout this manual. 

Instrument Options 

The Bio-Plex Pro and Bio-Plex Pro II Wash Stations are designed for strip-wise 
washing of 96-well microplates. They are designed primarily for the processing of 
Bio-Plex and other xMAP assays.   

The wash station is available in two versions. 

 

Wash Configuration 

Bio-Plex Pro 

Bio-Plex Pro II 

Vacuum filtration 

No 

Yes 

Magnetic separation  

Yes 

Yes 

 

Both wash stations are delivered with the standard carrier installed and the 
appropriate programs for performing your assays. Replace the standard carrier with 
the appropriate carrier for your assays as outlined on page 17. 

 

Which Wash Program Do I Use? On page 23 describes the standard wash programs 
necessary for the different assays.  

 

To run standard ELISAs or other plate-based assays, you must modify programs and 
plate definitions, using the HydroControl™ software available on the CD that ships 
with both instruments. This software is not needed for Bio-Plex or Bio-Plex Pro assay 
processing. Follow the instructions in Defining New Programs on page 53.  

 

Содержание Bio-Plex Pro

Страница 1: ...Instructions for Use for Bio Plex Pro Bio Plex Pro II Wash Stations Revision B Attention Use only the magnetic carrier which arrived with your instrument See page 17 for more details...

Страница 2: ...ii Bio Plex Pro and Bio Plex Pro II Wash Stations...

Страница 3: ...performing hands free wash programs on Bio Plex and other xMAP assays It contains instruction for the use of the instrument and is intended as a reference for the user It contains information about th...

Страница 4: ...iv Bio Plex Pro and Bio Plex Pro II Wash Stations...

Страница 5: ...ng and Installing a Plate Carrier 17 Changing the Vacuum Configuration 18 INSTALLING THE MICROPLATE 19 TURNING ON THE POWER 19 KEYPAD BASICS 20 Primary Menu 20 Secondary Menu 20 PRIMING THE INSTRUMENT...

Страница 6: ...OMPLETE INSTRUMENT KEYPAD FUNCTIONS 43 Alphabetical list of LCD text with explanations 45 DEFINING CUSTOM WASH PROGRAMS 48 WASH MODES 48 Washing Positions 49 ASPIRATING MODES 50 Normal AspirationMode...

Страница 7: ...ESSAGES 75 ERRORS WITHOUT MESSAGES 75 ERROR MESSAGES 75 Plate Error 75 Program Too Big Error 75 Transport Error 75 Manifold Arm Error 76 Sensor Defect Error 76 RS485 Error 76 PC Process Control System...

Страница 8: ...ates the possible presence of biologically hazardous material Proper laboratory safety precautions must be observed Attention Negative environmental impacts associated with the treatment of waste z Do...

Страница 9: ...nal Electrotechnical Commission IFU Instructions for Use in Inch IVD In Vitro Diagnostics IVD D In Vitro Diagnostics Directive kg Kilogram l L Liter m Meter mBar Millibar ml Milliliter mm Millimeter m...

Страница 10: ...Basic Operation and Maintenance 10 Bio Plex Pro and Bio Plex Pro II Wash Stations...

Страница 11: ...nd other xMAP assays The wash station is available in two versions Wash Configuration Bio Plex Pro Bio Plex Pro II Vacuum filtration No Yes Magnetic separation Yes Yes Both wash stations are delivered...

Страница 12: ...pport Only these recommended filter plates should be used with the Bio Plex Pro II when configured for standard Bio Plex or other polystyrene bead based xMAP assays Recommended microplates for vacuum...

Страница 13: ...no possibility of condensation causing a short circuit 1 Ensure that the on off switch in the rear panel of the instrument is in the off position 2 Connect the solution tubes L1 and L2 to the inlet co...

Страница 14: ...Basic Operation and Maintenance 14 Bio Plex Pro and Bio Plex Pro II Wash Stations Bio Plex Pro Installation Diagram...

Страница 15: ...Basic Operation and Maintenance Bio Plex Pro and Bio Plex Pro II Wash Stations 15 Bio Plex Pro II Installation Diagram...

Страница 16: ...II only Your Bio Plex Pro II is shipped with the liquid hoses attached Attach the tubing bracket to the left side of the instrument as shown in the pictures below Location of Tubing Bracket A tool is...

Страница 17: ...d the front of the instrument until it clears the guide bar Prime Tray Guide Bar Installing the plate carrier is the reverse of the procedure above 1 Carefully guide the plate carrier onto the guide b...

Страница 18: ...e of Enable The instrument must be turned off and then back on for the change to take effect OPTIONS EXIT OK EDIT LLD CHANNEL EXIT OK Select and press OK for OPTIONS System places you in the Options m...

Страница 19: ...ectly oriented position A1 of the plate corresponds to position A1 marked on the plate transport If using the vacuum plate carrier for polystyrene based assays see Changing the Plate Carrier on page 2...

Страница 20: ...ndary Menu If you choose OK when any of the primary menu options Programs Settings and Procedures are visible on the LED you will navigate to the secondary menus You may Select a choice from the secon...

Страница 21: ...as described above Alternately you may carefully use a syringe to inject 1 2 ml of water directly into the L1 port of the instrument reconnect the tubing and run a prime procedure PRIME OK CHANNEL 1...

Страница 22: ...are found in the Defining New Programs section on page 53 START OK SSSSSSSSSSSS OK RUN1 Program X EXIT OK PROGRAM OK Navigate to PROGRAM in the primary menu and press OK Use the keys under the and sym...

Страница 23: ...irst wash magnetic assay MAG x2 All other washes magnetic assay MAG x3 Prewash vacuum assay PREVAC First wash vacuum assay VAC x2 All other washes vacuum assay VAC x3 Third party kits which require 20...

Страница 24: ...pressure to the plate for the first vacuum cycle of the day Monitor your plate periodically to ensure complete removal of wash buffer before the next dispense cycle Blot the bottom of the filter plat...

Страница 25: ...isplayed RINSE DAY STOP END The manifold remains in the prime tray until END is pressed Press STOP to abort the procedure prime tray will not be aspirated Navigate to PROCEDURE in the primary menu and...

Страница 26: ...instrument on if you want to soak the manifold overnight Recommended during periods of heavy use Shut the instrument off if you do not wish to soak the manifold Rinse Night only Navigate to PROCEDURE...

Страница 27: ...ystem as outlined above When the rinsing procedure is completed the following message is displayed RINSE NIGHT STOP END The manifold remains in the prime tray until END is pressed Press STOP to abort...

Страница 28: ...id system if necessary Perform Rinse Day with wash buffer if the instrument is left to stand for a short time up to 2 hours Use distilled water if you anticipate longer intervals between uses Perform...

Страница 29: ...and clean each instrument needle by inserting the cleaning needle into the instrument needle and gently agitating to remove any possible debris Then rinse with water If you choose to remove the manifo...

Страница 30: ...are not answered in Basic Operation and Maintenance The reference section is provided for customers interested in more detail about the operation of the wash stations Provided you follow standard saf...

Страница 31: ...ty precautions such as wearing protective clothing and using approved laboratory safety procedures Note Suitable Compact Microplates The instrument supports the processing of flat round or v shaped bo...

Страница 32: ...ode 21640582 the recommended concentration is 1 ml of antifoaming agent for 1 liter of waste solution Use antifoaming agents and concentrations as recommended by corresponding manufacturers Intended U...

Страница 33: ...43 Use the appropriate standard programs Both instruments can also be used to process other plate based assays If you wish to do so you can leave the magnetic carrier in place If you have concerns abo...

Страница 34: ...hannel manifold USB Interface All connected devices must be approved and listed as per IEC 60950 1 Information Technology Equipment Safety or equivalent local standards Variable Volume of solution dis...

Страница 35: ...20 240 V 50 60 Hz Consumption 65 VA Physical Outside dimensions without Vacuum Filtration Width 275 mm Depth 366 mm Height 180 5 mm Width 10 8 in Depth 14 4 in Height 7 1 in Greater depth and height i...

Страница 36: ...ro and Bio Plex Pro II Wash Stations Description The diagram below shows the main components of the instrument Keyboard and Display Unit Manifold Arm Manifold Prime Tray Plate Support Guide Bar Power...

Страница 37: ...About the Instrument Bio Plex Pro and Bio Plex Pro II Wash Stations 37 Bio Plex Pro Dimensions...

Страница 38: ...About the Instrument 38 Bio Plex Pro and Bio Plex Pro II Wash Stations Bio Plex Pro II Dimensions...

Страница 39: ...late is located under the main power socket The company information on the top differs depending upon where it is purchased The nameplate also shows the model name the model part number and the serial...

Страница 40: ...hey should not be placed more than 1 m below the level of the instrument since this may result in instrument malfunction For information regarding outer dimensions and weight of the instrument see the...

Страница 41: ...h lid 6 Bundle of 2 tubes for connecting to the liquid bottles 5mm outer diameter 3mm inner diameter 7 Main power cable 8 Instrument manual 9 CD with software and support material 10 10 Bio Plex Pro f...

Страница 42: ...message is displayed If a Rinse procedure was aborted before the instrument was switched off the following message is displayed after the instrument initializes Press OK and then select a Rinse proced...

Страница 43: ...m each processing step can be identical or different from the previous step Adjustable soak time 1 second to 60 minutes and 59 seconds Variable shaking settings o High Linear shaking with a shaking fr...

Страница 44: ......

Страница 45: ...Defining New Programs Bio Plex Pro and Bio Plex Pro II Wash Stations 45 Alphabetical list of LCD text with explanations...

Страница 46: ...Defining New Programs 46 Bio Plex Pro and Bio Plex Pro II Wash Stations...

Страница 47: ...Defining New Programs Bio Plex Pro and Bio Plex Pro II Wash Stations 47...

Страница 48: ...ted in the program otherwise spilling can occur and the instrument may become contaminated WARNING When the instrument is busy do not touch the manifold After the instrument has been used the manifold...

Страница 49: ...ow and bottom position Move Overflow If Move Overflow MOVE OVER is selected the manifold moves step wise from the bottom position to the Overflow position during dispensing It is recommended for cell...

Страница 50: ...d bottom or V shaped bottom wells the aspirating needles are placed in the middle of the wells Only one aspiration position can be selected Round Bottom Wells V Shaped Wells Normal Aspirating Position...

Страница 51: ...e detachment of cells and increases the remaining cell percentage at the bottom of the wells The following example shows a typical wash program for working with adherent cells as it would appear in th...

Страница 52: ...to the next parameter Select PROGRAM END and press OK Set the volume to 450 l and press OK to continue to the next parameter set the channel to 1 and press OK to continue to the next parameter Set th...

Страница 53: ...ciency SOAK During a soak step the liquid remains in the wells for the set time with or without shaking VAC For collecting desired substances by vacuum filtration using filtration plates USER PROMPT U...

Страница 54: ...repeated up to 9 times C1 P1 ASP EXIT OK Press or to select the process step ASP DISP WASH SOAK PROGRAM END CX CYCLE where X is the number of the next cycle Press OK to define the selected process ste...

Страница 55: ...spiration or select NO to perform normal aspiration Press or to select the aspiration position BOTTOM OVERFLOW or CUSTOM Select required aspirating rate 1 3 where 1 is the weakest and 3 the strongest...

Страница 56: ...Select the volume of solution to be used 50 400 l in increments of 50 l Select and confirm dispensing step DISP CX Cycle Number PX Program Step Number CX PX DISP EXIT OK VOLUME 300 l EXIT OK CHANNEL...

Страница 57: ...ispensing Position Diagrams Z Position Bottom Z Position Custom Z Position Overflow Position Diagrams MOVE OVER Overflow Position Movement of manifold MOVE CUST Custom Position Movement of manifold Bo...

Страница 58: ...of solution to be used 50 3000 l in increments of 50 l Select and confirm wash step WASH CX Cycle Number PX Program Step Number Select required flow rate drip to 500 l sec Select required aspirating...

Страница 59: ...select the next program step Program End or a new cycle Process Step User Prompt Select and confirm User Prompt strip on required position in current program CX PX USER PR EXIT OK Process Step Vacuum...

Страница 60: ...o return to the previous step Cycle 1 will be shown Press to show the program steps of this cycle Press OK to show the cycle number POS OVERFLOW EXIT OK VOLUME 300 l EXIT OK CHANNEL 1 EXIT OK H SPEED...

Страница 61: ...d the following message is displayed PROGRAM IS LOCKED The program can only be unlocked via the HydroControl software by users with the appropriate rights Settings Menu The Settings menu has the follo...

Страница 62: ...spiration can be defined The instrument moves to the predefined position Use the and keys to change the position Confirm with OK The next position can then be selected Press OK to save the defined pos...

Страница 63: ...edures Prime Start prime procedures Empty Prime Tray Aspirate liquid out of the prime tray Vacuum Filtration Prepare the vacuum pressure to prevent delays during the running of a wash program The Proc...

Страница 64: ......

Страница 65: ...instrument is to rinse the liquid system with distilled water before the instrument is left to stand or switched off at the end of each day The manifold should be removed and cleaned thoroughly at le...

Страница 66: ...be removed from the instrument Autoclaving max 130 C max five times manifold must be removed from the instrument Do not autoclave manifolds with the Process Control option Reinstall the manifold if ne...

Страница 67: ...quid or foam may occur when the Instrument is operated improperly such as Microplate used does not match installed manifold The strip plate positions do not match the positions defined in the program...

Страница 68: ...ifold WARNING After the instrument has been used the manifold may be infectious Before the manifold is removed it must be thoroughly disinfected It is advisable to adhere to applicable safety precauti...

Страница 69: ...at least once every 6 months or whenever needles become blocked 1 Lift the manifold arm and remove the black manifold arm guard plate by removing the three screws using the Allen key provided 2 Lift t...

Страница 70: ...es in the bracket 3 Tighten the manifold into place using the two screws using the Allen key provided 4 Fit the dispensing tubes marked blue onto the top connector on the rear of the manifold 5 Fit th...

Страница 71: ...t the instrument is thoroughly disinfected before it is removed from the laboratory or any servicing is performed on it Before the instrument is returned to the service center for service or repair it...

Страница 72: ...ing procedure should be used to disinfect the instrument 1 Prepare an autoclave bag for all disposables used during the disinfection procedure label it with autoclave tape and disinfect the material u...

Страница 73: ...e treatment of electrical and electronic equipment waste Do not treat electrical and electronic equipment as unsorted municipal waste Collect waste from electrical and electronic equipment separately...

Страница 74: ...on the applications parts of the Instrument may have been in contact with biohazardous material Make sure to treat this material according to the applicable safety standards and regulations Always cle...

Страница 75: ...s Plate Error If the plate sensor does not recognize the plate or no plate is inserted on the plate carrier the following message is displayed NO PLATE OK Press OK and insert the plate correctly on th...

Страница 76: ...that particular type of microplate Sensor Defect Error The following message error is displayed when the dispensing pump or the dispensing sensor is defective SENSOR DEFECT OK Press OK to remove the e...

Страница 77: ...Your system is not equipped for process control so it must be deactivated The following directions describe how to check and disable a PC setting Remember to exit to the Primary Menu before performing...

Страница 78: ...ur system This is an advanced function for which your system is not equipped All channels should be deactivated for LLD The following directions describe how to check and clear all channels of an LLD...

Страница 79: ...Bio Plex Pro and Bio Plex Pro II Wash Stations 79 Declaration of Conformity...

Страница 80: ...ad 20 Maintenance 28 Menus and the keypad 20 Navigating 20 Options 62 Overview 20 Primary 20 Procedures 63 Program 53 Quick Guide 24 Secondary 20 Settings 61 Microplates Installation 19 Recommended 12...

Страница 81: ...Bio Plex Pro and Bio Plex Pro II Wash Stations 81...

Страница 82: ...82 Bio Plex Pro and Bio Plex Pro II Wash Stations...

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