
55
Appendix A. Trouble Shooting
Problem
Cause
Solution
Device
Not powering on
Power switch is in off position.
Check the power switch on back of unit.
No power from outlet.
Check the power source.
Bad power cable.
Replace the cable.
Inaccurate result
Stain solution has expired or
been contaminated.
Use new stain solution or filter the solution.
Too many aggregated cells.
Try again, pipette the cell mixture gently to mix the
cells before adding to the slide chambers.
(check the cell image for excessive cell clumping or
agglomerates)
Sampling error
✓
Repeat steps of properly pipetting the cell
mixture for the staining process.
✓
Before sampling the cell suspension, gently
resuspend the cells at least 6 times by gently
pipetting up and down
✓
Sample from the middle of the cell suspension
tube, not near the surface or the bottom.
Bubbles in slide chambers
Pay careful attention to avoid bubbles when
pipetting and loading samples into the slide
low cell concentration
(
≤
5 x 10
4
)
Try again using the Precise mode.
Cell size is smaller than 10µm
or around 10µm.
Change the gating size parameter in the histogram.
The ratio of trypan blue in the
sample is too high or too low..
Mix cell suspension and trypan blue at a 1:1
volume ratio.
Too Bright or dark cell image
Mix cell suspension and trypan blue 1:1.
If the problem is not resolved, contact Accuris or
your local distributor.
The grid pattern or line is visible
in the result images.
Try again using another slide.
If the problem occurs frequently, contact your local
distributor.
Exported data or
Report is
corrupted
The USB memory was removed
before displaying the
notification message
Wait until after the notification message appears,
then remove the USB memory.
USB memory
Not Connected to
device
The USB memory is formatted
to ex-FAT or NTFS file system.
Use USB memory included with the QuadCount
package or another formatted to FAT32 file system
Содержание Accuris
Страница 2: ...2...
Страница 31: ...31 STEP 4 Press Back button STEP 5 Ready to count JurkatT JurkatT NIH Hela U937 Channel ID...
Страница 39: ...39 STEP 3 Press Data icon STEP 4 Review the number of Live cells and Viability 60...
Страница 47: ...47 Setting screen FN...
Страница 53: ...53 STEP 3 Press Set button to save the adjusted values STEP 4 Return to the Home screen 22...
Страница 59: ...59 Appendix D Example and explanation of PDF report...