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Contents

Chapter 1: About this guide

Conventions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6

Technical support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Chapter 2: System overview

Product description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10

Principles of operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12

Control panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13

Sheath plenum enclosure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16

Specifications and requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

Chapter 3: Operation

Starting up the system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20

Shutting down the system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Responding to alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

Chapter 4: Maintenance

Replacing the sheath cubitainer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26

Replacing the waste cubitainer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27

Flushing the BD LSRFortessa system . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30

Flushing the BD LSRFortessa system with a BD HTS . . . . . . . . . . . . . . . . . . . . . 33

Replacing the air filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36

Changing the sheath filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

Sensors and tubing locations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41

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Содержание FACSFlow Supply System

Страница 1: ...d Company BD Biosciences 2350 Qume Drive San Jose CA 95131 USA bdbiosciences com ResearchApplications bd com BD Biosciences European Customer Support Tel 32 53 720 600 help biosciences bd com BD FACSF...

Страница 2: ...every precaution to ensure accuracy BD Biosciences assumes no liability for any errors or omissions nor for any damages resulting from the application or use of this information BD Biosciences welcome...

Страница 3: ...ations and requirements 17 Chapter 3 Operation 19 Starting up the system 20 Shutting down the system 22 Responding to alarms 22 Chapter 4 Maintenance 25 Replacing the sheath cubitainer 26 Replacing th...

Страница 4: ...BD FACSFlow Supply System User s Guide iv Chapter 5 Troubleshooting 43 Troubleshooting 44...

Страница 5: ...ACSFlow supply system The BD FACSFlow Supply System User s Guide is intended for laboratory personnel and flow cytometry operators Application specific instructions are not included in this guide This...

Страница 6: ...used in this guide to alert you to potential hazards Safety symbols Symbol Meaning Caution alert Identifies a hazard or unsafe practice that could result in data loss material damage minor injury seve...

Страница 7: ...cting technical support If additional assistance is required contact your local BD Biosciences technical support representative or supplier When contacting BD Biosciences have the following informatio...

Страница 8: ...This page intentionally left blank...

Страница 9: ...ystem overview This section includes these topics Product description page 10 Principles of operation page 12 Control panel page 13 Sheath plenum enclosure page 16 Specifications and requirements page...

Страница 10: ...y system to the sheath plenum The photo shows the BD FACSFlow supply system and its associated parts The system includes BD FACSFlow supply system unit Four liter sheath plenum for the cytometer store...

Страница 11: ...cription 1 Power switch 2 Cabinet base 3 Air filter 4 Waste cubitainer probe 5 Probe holder 6 Sheath cubitainer probe 7 Plenum Caution Do not move the BD FACSFlow supply system by pulling on the probe...

Страница 12: ...probes with a fluid level sensor that extends into the plenum When the probe in the sheath plenum detects a specified low level it signals the BD FACSFlow supply system to pump sheath fluid from the 2...

Страница 13: ...nction of the controls and indicators on the control panel You can access and monitor all of the BD FACSFlow supply system functions from the control panel Control buttons The following figure and tab...

Страница 14: ...s off the audible alarm The indicator light remains flashing until the situation is remedied 2 Prime Pumps sheath fluid to the sheath plenum until it reaches the default operational level 3 Restart Af...

Страница 15: ...4 Pump Fill Steady Yellow Sheath fluid is being pumped from the cubitainer to fill the cytometer sheath plenum 5 Drain Pump Steady Yellow The drain pump is in use 6 System On Steady Green The system i...

Страница 16: ...ntroduction This topic describes the sheath plenum enclosure Description The sheath plenum enclosure holds the sheath plenum and the sheath filter The following figure shows the enclosure Caution The...

Страница 17: ...page 39 Specifications and requirements Introduction This topic lists the specifications and requirements for the product Physical Height 57 cm 22 4 in Width 62 cm 24 4 in Depth 73 cm 28 7 in Weight...

Страница 18: ...BD FACSFlow Supply System User s Guide 18 Operating environment Temperature 16 C 29 C Humidity 10 90 relative humidity at 35 C non condensing Air filtering no excessive dust and smoke...

Страница 19: ...3 Operation This section includes these topics Starting up the system page 20 Shutting down the system page 22 Responding to alarms page 22...

Страница 20: ...an run dry Procedure It is important to perform the following steps in the order given This will allow the pump in the BD FACSFlow supply system to fill the sheath plenum without creating back pressur...

Страница 21: ...dicator light and then press and hold down the Prime button for one half second Use only the Prime button if there is air in the sheath fluid supply line If the Prime button does not activate the pump...

Страница 22: ...cytometer shutdown procedure 2 Shut off the BD FACSFlow supply system by toggling the power switch to the Off position after you have turned off the cytometer More information Starting up the system...

Страница 23: ...ensor probe into a 500 mL beaker filled with a fresh 10 bleach solution and let the probe soak for a few minutes For instructions on preparing the bleach solution see Replacing the waste cubitainer pa...

Страница 24: ...sciences Procedure Sheath Empty alarm The Sheath Empty alarm is triggered when the sensor probe detects that the sheath cubitainer is empty To respond to a Sheath Empty alarm 1 Press the Alarm button...

Страница 25: ...ath cubitainer page 26 Replacing the waste cubitainer page 27 Flushing the BD LSRFortessa system page 30 Flushing the BD LSRFortessa system with a BD HTS page 33 Replacing the air filter page 37 Chang...

Страница 26: ...from hitting the bottom of the cubitainer Procedure To replace the sheath cubitainer 1 Loosen the sheath sensor probe cap assembly and carefully remove the sensor probe from the sheath cubitainer keep...

Страница 27: ...eplace the waste cubitainer whenever it gets close to being full You can use BD FACSClean at full strength as a replacement for 10 bleach Preparing 10 bleach solution Prepare a 10 bleach solution usin...

Страница 28: ...r probe from the waste cubitainer 4 Place a cap set aside from a new sheath cubitainer installed in the system onto the waste cubitainer and tighten it Caution Biohazard All biological specimens and m...

Страница 29: ...label the cubitainer as WASTE and affix a biohazard label onto the cubitainer 8 Discard the waste according to federal state and local regulations Item Description 1 Neck support color 2 Waste cubita...

Страница 30: ...tart More information Flushing the BD LSRFortessa system page 30 Replacing the sheath cubitainer page 26 Flushing the BD LSRFortessa system Introduction This topic describes the procedure for flushing...

Страница 31: ...s steel container near the cytometer Note In the next two steps use the tubing lines that are dedicated to the stainless steel container 4 Connect the air line green from the stainless steel container...

Страница 32: ...e STNDBY button lights amber press the PRIME button again 6 Install a tube with 3 mL of 10 bleach or full strength BD BD FACSClean solution on the SIP and put the tube support arm underneath the tube...

Страница 33: ...rinse any remaining bleach out of the stainless steel container when the procedure is completed to avoid corrosion 16 Reconnect the air and fluid lines coming from the sheath plenum More information...

Страница 34: ...ntainer The cytometer system is shipped with a stainless steel container for sheath fluid This container is replaced by the sheath cubitainer when the BD FACSFlow supply system is installed Save the s...

Страница 35: ...ess steel container near the cytometer Note In the next two steps use the tubing lines that are dedicated to the stainless steel container 5 Connect the air line green from the stainless steel contain...

Страница 36: ...dialog is displayed while cleaning is in progress Cleaning can take up to 30 minutes 8 Remove the stainless steel container and fill it with DI water 9 Reinstall the stainless steel container and clic...

Страница 37: ...n This topic describes the procedure for replacing the air filters An air filter is located on the cap assemblies of the sheath and waste cubitainers Procedure To replace an air filter 1 Remove the ai...

Страница 38: ...nsert a new air filter into the silicone tubing 3 Verify that the cap assembly on each cubitainer is tightened Item Description 1 Waste sensor probe 2 Air filter 3 Silicone tubing 4 Cap assembly 5 She...

Страница 39: ...ilter Introduction This topic describes how to change the sheath filter The sheath filter is connected in line with the sheath line It filters the sheath fluid as it comes from the sheath container Wh...

Страница 40: ...quick disconnect on both sides of the filter assembly and pulling it out of the holder on the sheath plenum enclosure 3 Over a sink or beaker Remove the vent line from the filter and set it aside Remo...

Страница 41: ...th line to the filter assembly by squeezing the quick disconnect 4 Attach the cytometer fluid line to the filter assembly via the quick disconnect 5 Install the filter assembly into the holder on the...

Страница 42: ...m The tubing has color coded connectors that plug into the BD FACSFlow supply system Caution Biohazard Do not disconnect the tubing from the back of the BD FACSFlow supply system or loosen the caps fr...

Страница 43: ...n Color Function 1 black AC plug 2 black 1 A T fuse 3 silver Waste cubitainer sensor connector 4 blue Waste cubitainer tubing connector 5 red Waste container tubing connector 6 silver Waste container...

Страница 44: ...BD FACSFlow Supply System User s Guide 44...

Страница 45: ...5 Troubleshooting This section includes this topic Troubleshooting page 44...

Страница 46: ...osciences service representative Sheath plenum not refilling when it should The sheath probe in the sheath plenum is wet or otherwise not functioning correctly Remove the sheath probe and dry it off t...

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