Azure™ cSeries User Manual
Page 13
5. Select
CAPTURE
. The channels will be captured sequentially.
6. The resulting image will be displayed in the gallery tab.
3.6 Custom Applications
The custom tab allows the users full flexibility when acquiring
their image.
LOAD PROTOCOL
– Load Previously saved
protocols, for quick image capture
SAVE PROTOCOL
– If you have a custom
setting you plan to use multiple times, save
it, for quick recall in the future.
Imaging workflow:
1. Select the sensitivity/resolution for your setting. To use the full camera resolution, select
LOWEST
.
2. Select the light sources you wish to use.
a. If you wish to take a single channel image, select a light source next to
GRAYSCALE
.
b. If you wish to take a multichannel image, make sure the imaging time next to Grayscale is
set to
0
. Then, select the light sources you would like to image, next to the color channel
you would like them assigned to. Set the exposure time for each channel.
3. Select the aperture setting: the smaller the number, the wider the aperture. For
chemiluminescence, we recommend 0.95 for fastest light gathering. For all other applications,
2.8 is recommend for improved depth of field and focus.
4. Select the focus setting:
a. For chemi applications on the Blot Tray on the UV transilluminator, we suggest 2600.
b. For chemi applications on the Blot Tray on the chemi shelf, we suggest 8400.
c. For NIR applications, we suggest 3700-4200.
d. For RGB applications, we suggest 2400-3000.
e. For gel applications, we suggest 2400.
5. Select the number of frames.
6. Save your protocol if you think you will use it in the future.
7. Hit
CAPTURE
. The resulting imaging will be displayed in the gallery tab.
3.7 Save Acquired Image
Users are discouraged to store image files on the tablet since
its disk space and memory resources are limited. Please use
a USB storage device, or if the system is networked, save to
a network location. There are three USB port, one in the front
and two on the right side panel.