Azure™ cSeries User Manual
Page 10
3.2
B
Band Excision and Light Safety Switch
The light safety interlock switch is located on the front edge of the system and is visible when the door
is open. Normally, if UV light is being used, the cabinet automatically shuts off the UV light source when
the door is opened.
The
BAND EXCISION
button was created to override the safety switch for situations when a
user needs to cut bands out of their UV excitable gel.
Care must be taken to avoid eye and skin exposure to the UV radiation.
WARNING: ALWAYS WEAR APPROPRIATE PROTECTION WHEN EXPOSED TO UV LIGHT. The
use of protective eyeglasses, mask, and/or gloves is strongly recommended.
After selecting the Band Excision button, the user will be prompted to pull out the override safety
interlocked switched, pictured here:
Once the user pulls out the switch, and selects
OK
from the screen prompt, the light will remain on for
5 minutes, or until the band excision switch is pressed again. Capturing an image, or selecting another
light source will also turn the light off.
3.3 Chemiluminescent Applications (c300 systems and above)
SENSITIVITY
– Changing the sensitivity
settings changes both the sensitivity
and the resolution of the camera
and image. At the Lowest sensitivity
setting, you will be taking a full
resolution image with the camera.
And the Highest sensitivity setting, the
resulting image will be 0.3 MP image.
The resolution is displayed next to the
sensitivity settings.
CUMULATIVE
– Selecting Cumulative will tell the software to take up to 10 images at the
time interval you selected for exposure times. For example: if you set an exposure time
of 1 minute, it will display 10 images, every 1 minute. The first image displayed will be a 1
minute exposure. The second image displayed, at the 2 minute interval, will be a sum of
the first two images. The finally, tenth image, will be the sum of 10, 1 minute images. You
can view the images in the gallery tab while the next image is being acquired.
MARKER
– When taking a single channel image, if marker is selected, after capturing a
chemi image, the system will take a white light image of the blot. The resulting image
displayed in the gallery tab will show the chemi blot in the green channel, and the
white light image in the red channel. This will allow you to overlay your signal over the
image of your blot. Note: Marker feature works optimally when taken on the bottom, UV
transilluminator shelf position.
For all blots, use the blot tray which is stored in the door. The blot tray provides a dark background to
improve the contrast on the blot image.