Azure™ cSeries User Manual
Page 9
3.2 Gel Applications
For applications that include EtBr, SYBR
®
Green, Coomassie
Blue or similar, select the Gel tab. You will see the following user
interface:
AUTOEXPOSE
– Selecting autoexposure as
the exposure time will allow the software
to capture the image for you, using the full
dynamic range of the camera.
CAPTURE
– when you are ready to capture
your image, the capture button tells the system to start acquiring an image.
3.2
A
Imaging Workflow
1. Select a light source. You can select from UV 302, UV 365, EPI Blue or Visible. The correct
emission filter will be selected automatically.
• UV 302 – For dyes that are UV excitable, UV 302 will be the brightest and best light source.
• UV 365 – For cutting bands out of a gel, UV 365 reduces the nicks that might be introduced
into the DNA by UV 302.
• EPI Blue- Select this light source for gels that are stained with dyes that excite in the blue
range.
• Visible – For dyes that are visible without additional excitation, use the Visible light source.
When selected, the software will prompt you to use the Orange Tray, which converts the UV
light into bright visible light to provide contrast to your image.
2. Place the sample on the UV transilluminator, or on the Orange Tray for Visible samples.
3. Select an imaging time.
a. Manual Imaging times are entered using our keypad. For gels,
we``` recommend setting exposure time to less than 1 minute.
• For 20 seconds, hit
2
then
0
then
S
. To select 1.5 seconds,
type
1
then
S
, and then
5 0 0
and
MS.
• To erase what you done, hit the
C
button, for clear.
b. Or, you can select
AUTOEXPOSE
which will calculate the best
imaging time for you image. Selecting autoexpose will grey out the keypad, enabling you to
simply press capture.
c. Maximum exposure time is 30 seconds.
4. Hit
CAPTURE.
The software will direct the camera to collect the image, and the final image will be
displayed in the Gallery tab.