Applied Photophysics Chirascan LD, Руководство пользователя

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Document 4207Q258 V3.00 January 2018 Page 28 of 30
Chirascan Couette Cell LD Accessory User Manual
3.7 Example: Calf thymus DNA
Linear dichroism spectra for calf thymus DNA, Sigma-Aldrich product D1501, at shear rates from 0 to 10,200
s
-1 , are shown in Figure 3.13 . The spectrum for a shear rate of 0 s -1 was used as the baseline. The conditions
used are shown in Table 3.1 .
Figure 3.13: LD spectra for calf thymus DNA at various shear rates
Sample Calf Thymus DNA
Concentration 0.13 mg/ml
Buffer Water
Wavelength range 180 to 360 nm
Wavelength step 1 nm
Bandwidth 1 nm
Time-per-point 0.5 s except shear rates 120 and 240 s which were 1 s
Pathlength 0.5 mm (total)
Temperature Ambient (

20.5

C)
Repeats 3 (shear rates 0 to 30 s
-1
)
2 (shear rates 60 to 10,200 s
-1
)
Shear rates 0 (baseline), 1, 2.4, 6, 12, 30, 60, 120, 240, 480, 960, 1920, 3840,
6000, 7800, 9000, 10,200 s
-1
Table 3.1: Conditions used for the LD spectra of DNA at various shear rates