Apostle MagTouch 1000, Руководство пользователя

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Plate
Position
Plate
Type
Plate
ID
Content
Reagent volume for each
well
1
2
3
4
5
96 well
plate
96 well
plate
96 well
plate
96 well
plate
96 well
plate with
comb
Bind
EtOH
EtOH
Elution
Comb -
Elution Solution
80% Ethanol
80% Ethanol
Sample
Lysis/Binding Solution
(EtOH added)**
-
85
µ
L
500
µ
L
500
µ
L
200
µ
L
520
µ
L
*Check 96 well plate integrity before loading reagents, to avoid leakage of certain wells.
**Always mix the bottle of Lysis/Binding Solution well right before pouring into reagent reservoir, which is
used to load plates, as the magnetic beads can precipitate and compromise results. Also vortex the
reagent reservoir for Lysis/Binding Solution before adding more Lysis/Binding Solution.
C. Automated viral Total NA isolation on Apostle MagTouch 1000
Automation Platform
1. Initialize the instrument, then press the [Quick Start] button.
2. Select the built-in [viral RNA 2.0] program and place the plates into the Apostle
MagTouch 1000 as indicated on the instrument display. Make sure that the plate is
placed steadily in the correct orientation.
Ensure that the A1 well is in the upper
right corner of the plate holder on turntable.
3. After placing all the plates, press the icon on the interface to start the
program.
4. When the program is complete, collect the purified viral RNA from the elution
plate.
5. For long term storage, store the samples at -80 C.
o
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