Introduction
Congratulations on the purchase of your new Reichert
r
2
i300
reflected light refractometer.
This manual describes the proper operation and care of this state of the art instrument. The
purpose of this instrument is to aid quality control personnel in food and beverage,
pharmaceutical, industrial fluids, chemical and petrochemical applications. The
r
2
i300
’s simple, yet advanced features, make it a well suited and widely used instrument.
The
r
2
i300
refractometer is a high precision optical instrument. To obtain the best possible
accuracy, it is absolutely necessary to maintain the instrument. The precision and accuracy of
a refractive index measurement is largely dependent upon operator skill, cleaning practices,
temperature control, accuracy of calibration standards, chemical stability and the physical char-
acteristics of the sample. Use of good laboratory practice is essential. Reichert recommends
that daily calibration with distilled water be performed as a minimum requirement. To assure the
longest life of your
r
2
i300
, always store the instrument with the prism surfaces and instrument
clean and dry to prevent premature failure of the prisms and seals due to any harmful effects
from sample residue.
The instrument is programmed to read out directly the refractive Index, percent solids and the
temperature of samples. The percent solids scale is based upon the ICUMSA conversion tables
relating refractive index to percent solids concentration for sucrose solutions. For non-sucrose
solutions, they may be read directly in the refractive index mode (non-temperature corrected), or
in the percent solids mode provided that the conversion value is known.
For product specific measurement, custom channels also may be programmed into the
r
2
i300
.
Custom channels allow the instrument to read out directly in refractive index or percent solids
temperature compensated values. For more information regarding custom channel programming,
contact Reichert Analytical Instruments Technical Service.
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