Amersham Biosciences AKTA design P-960, Руководство пользователя, Страница: 30 / 40

5
Troubleshooting
30
Pump P-960 User Manual 18-1172-73 Edition AA
8 Switch sample valve V5 to the next sample inlet tubing to be filled.
Note: To empty the syringe, switch valve V5 before removing the
syringe to prevent sample from flowing back to the vessel.
9 Repeat step 6 and 7 for the remaining sample inlet tubings.
To fill the buffer inlet tubing in port 8:
1 Set the sample valve V5 to port 8.
2 Draw buffer with the syringe until the liquid level has passed
through the sample pump.
The check valves in the sample pump will prevent the liquid from
being withdrawn when removing the purge tubing.
3 Disconnect the purge tubing.
4 Fit the original connector to the port.
5 Flush the sample flow path tubing with buffer to remove any
trapped air bubbles according to the procedure in section 5.2.1.
Procedure for system not including sample valve V5
1 Put the sample inlet tubing in a buffer vessel.
2 Disconnect the connector fitted to the port that will be used for the
purge tubing (see Table 5-2).
3 Connect the purge tubing to the same port.
4 Set the injection valve V1 to LOAD.
5 Draw buffer with the syringe until the liquid level has passed
through the sample pump.
The check valves in the sample pump will prevent the liquid from
flowing back when removing the purge tubing.
6 Disconnect the purge tubing.
7 Fit the original connector to the port.
8 Flush the sample inlet tubing with buffer to remove any trapped air
bubbles according to the procedure in section 5.2.1.
9 Gently move the sample inlet tubing to the sample vessel.
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