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1200 Series Handheld Control Module Users’s Guide

115

Running an Isocratic Analysis

9

Preparing the LC System

Preparing the LC System

1

For the isocratic pump, fill the solvent bottle with the mixture of LC-grade 
bidistilled water (35 %) and acetonitrile (65 %). For the binary or 
quaternary pump, fill one solvent bottle with bidistilled water (channel A) 
and the other with acetonitrile (channel B).

2

Turn on the detector lamp and pump using the On/Off button [F7] in the 
Analysis screen. (Use Action keys then to select the module)

3

For the quaternary pump, turn on the degasser by pressing the line-power 
switch.

4

Purge the pump. For more information see 

Chapter 3

, “Using the Pump”.

5

Allow the detector at least 15 minutes to provide a stable baseline. 

6

Fill the contents of a Agilent Technologies isocratic standard sample 
ampoule into a vial and seal the vial with a cap. Place the vial in position 1 
of the autosampler tray.

7

Pump the water/acetonitrile (35/65 %) mobile phase through the column for 
10 minutes at a flow rate of 2 ml/min.

Содержание G1323A

Страница 1: ...A Agilent 1200 Series Handheld Control Module User s Guide ...

Страница 2: ...ses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as ...

Страница 3: ...e Users s Guide 3 In This Guide This book describes how to operate the Agilent 1200 Series modules and systems for HPLC using the control module Solvent cabinet Vacuum degasser Pump Column compartment Autosampler Detector Control module ...

Страница 4: ... Agilent 1200 Series Control Module This chapter gives an overview over the Agilent 1200 Series control module 2 Working with the Control Module This chapter describes how to use the Agilent 1200 Series control module Part 2 Using the Agilent 1200 Series Modules This part describes how to use the individual HPLC modules to run isocratic gradient and multiple vial analyses using a single method or ...

Страница 5: ...LC System With Control Module This part describes how to run isocratic gradient and multiple vial analyses using a single method or more than one method 9 Running an Isocratic Analysis This chapter describes how to analyze the Agilent Technologies isocratic standard sample using a single injection analysis 10 Running a Gradient Analysis This chapter describes how to analyze the Agilent Technologie...

Страница 6: ... Users s Guide A Appendix This chapter contains safety information Related Documents Each HPLC module is supplied with a Reference Manual or User Manual The control module is supplied with the following User s Guide Software Overview Guide ...

Страница 7: ...er key 21 Navigation keys 21 Action keys 21 Selection keys 21 Direction keys 21 Numeric Alphanumeric keys 22 Control Module Software 23 The Control Module User Interface 23 Analysis Screen 23 System Screen 27 Status Screen 29 Samples Screen 30 Plot Screen 31 Control Module Versions 32 2 Working with the Control Module 35 Installing the Control Module 36 General Functions 37 Turning On Off LC Syste...

Страница 8: ...ifying a Method Name 42 Protecting a Method 43 Saving a Method 44 Deleting a Method 45 Exchanging Methods With the PC Card 45 Transferring Methods Between LC Systems 46 Time Programming 47 Automating Analyses 48 Displaying Data Graphically 50 Selecting Signals 50 Rescaling the Plot Screen 51 Toggling Signals 52 Displaying Exact Signal Values 52 Print Graph 52 Logbooks 53 Maintenance Logbook 53 Err...

Страница 9: ...l RS 232 59 MIO 59 BCD 59 External Contacts 59 Firmware Updates 60 Coexecution with Agilent ChemStation 61 Features 61 Restrictions 62 3 Using the Pump 63 Turning the Pump On and Off 64 Entering Settings 64 Purging the Pump 64 Purging Procedure 65 Adjusting Compressibility 66 Adjusting Stroke Volume 68 Automatic Shut down 68 Troubleshooting the Pump 68 Tracking Pump History Pump EMF Limits 69 Rese...

Страница 10: ...Optimizing Autosampler Performance 78 Setting up the Thermostatted Autosampler 79 Aligning Transport Arm Gripper 80 Tracking Autosampler History EMF limits 81 Resetting the Autosampler 81 Reset to Default Settings 81 Troubleshooting the Autosampler 82 6 Using the Manual Injection Valve 83 About the Injection Valve 84 Making An Injection 85 Completely Filling the Sample Loop 85 Partially Filling th...

Страница 11: ...Detector 95 Tracking Detector History 96 Resetting the Detector 97 Lamp Ignition Routine VWD only 98 Configuring the Analog Channel Output 99 8 Using the Column Compartment 101 Turning the Column Compartment On and Off 102 Entering Settings 103 Configuring the Column Compartment 104 Configuring the Column ID Module 105 Selecting Separated or Combined Mode 106 Selecting the Column Switching Valve o...

Страница 12: ...g Settings in a Method 128 Observing the Chromatogram 129 Starting the Analysis 131 11 Running Multiple Vial Analyses 133 Analyzing Multiple Vials Using the Same Method 134 Analyzing Multiple Vials Using Different Methods 135 Single Level Calibration Sequences 137 Multiple Level Calibration Sequences 139 Recalibrating With the Same Group of Standards 139 Recalibrating With Multiple Groups of Stand...

Страница 13: ...njector Program 150 Entering Injector Program Settings 151 Injector Program Lines 152 Saving the Method 153 13 Maintaining the Control Module 155 Control Module Parts 156 A Appendix 157 Safety Information 158 General 158 Operation 158 Safety Symbols 159 Radio Interference 161 Test and Measurement 161 Agilent Technologies on Internet 161 Index 163 ...

Страница 14: ...14 1200 Series Handheld Control Module Users s Guide Contents ...

Страница 15: ...200 Series Handheld Control Module User s Guide Agilent Technologies 1 The Agilent 1200 Series Control Module Control Module Features 16 Control Module Keys 17 Control Module Software 23 Control Module Versions 32 ...

Страница 16: ... Define automated analyses including methods timetables injector programs method sequences and automated calibration settings using the control module Protect your method from any inadvertent keyboard changes by setting method protection Use PC cards to store and transfer methods and sequences between Agilent1200 systems Monitor all operations and error events using the self updating logbooks Use ...

Страница 17: ... values or alphabetic characters Direction keys move the cursor in the screen display Navigation keys access different software areas Esc key reverses current input entry or action Numeric keys enter numbers 0 to 9 Action keys execute specific operations and access Plot screen m menu key provides navigational shortcuts and actions Alphanumeric keys press numbers 1 to 9 multiple times e g 1 A B C 2...

Страница 18: ...er section or function buttons F6 F8 in the right hand section that can be accessed with the corresponding Navigation for menus and Action for functions keys NOTE In this context the expression button will always refer to a menu or function shown on the display whereas key refers to the actual keys on the keyboard The key corresponding to a certain button is shown in brackets F1 F8 ...

Страница 19: ...her information By pressing the Enter key you can display screens providing more specific information about the underlined word Some words have a dotted underline which indicates there is an available definition of the word By pressing Enter you can display a small overlay screen with information You can remove this overlay screen by pressing Enter To exit from the online information system press ...

Страница 20: ...lanation purposes Press the Esc key to exit the menu See the Quick Reference Guide on the availability of context sensitive menus In Figure 3 Analysis Screen context menus are shown Depending on the active parameter entry field context sensitive menus provide different options The Esc key The Esc Escape key allows you to exit the current window or screen and leads you back to the last window or sc...

Страница 21: ...igation keys always correspond to a button displayed above them on the screen The menus accessed via the buttons vary according to the screen you are working with In some cases pressing a button will cause a list box to appear From there you must make a choice in order to proceed Action keys The 3 Action keys F6 F8 trigger a variety of functions The available functions depend on the screen you are...

Страница 22: ...llow you to enter numeric values in parameter entry fields In certain fields where alphabetical characters may be entered you can use the Numeric Alphanumeric keys to do so Pressing them several times in sequence changes the current value according to Figure 4 Figure 4 Keypad of the Control Module 7 STU 6 PQR 5 MNO 1 ABC 4 JKL 3 GHI 2 DEF 0 9 YZ_ 8 VWX ...

Страница 23: ...roceeding From there you have to make a choice in order to go on either by using the up down Selection keys or by pressing the corresponding number on the Numeric Alphanumeric keys The latter will give you quicker access to the choices in most cases Mostly these pop up menus will distinguish between the different modules in your system or in the case of the Views button F5 between screens Analysis...

Страница 24: ...ses and Views other screens Samples easy access on vials Status most important parameters during analysis and System control configuration tests and records Figure 6 Analysis Screen Information Autosampler parameter vial range setting number of injections setting screen name modification flag module status Pump parameter flow rate setting system pressure solvent composition Autosampler parameter i...

Страница 25: ...y in the Analysis screen prompts context sensitive menus depending on the active parameter entry field see Figure 3 on page 20 The Module Status on the top right hand side of the screen and on top of the individual module tabs indicates whether the system module is ready or not indicates the system is ready indicates the system module is not ready only applicable for individual modules indicates t...

Страница 26: ...Timetable Defaults Pump Flow Pressure Solvent Composition Autosampler Inj Program Inj Vol Draw Speed Optimization Column Comp Temperature Left Right Temp Range DA MW VW FL or RI Detector Wavelengths Peakwidth Auto Balance Spectrum Handle methods on modules or PC cards Save As Load Print User ID Comment Combine methods for automated analysis End Actions Calibration Manage PC card Sequence Logbook A...

Страница 27: ...book together with the LC system and module status The EMF Early Maintenance Feedback icon will flash to indicate when an EMF limit is exceeded The screen provides access to LC system and module controls for example pump on off lamp on off heater on off and injector reset LC system and module configuration module tests and LC system and module records Figure 8 System Screen Information System logb...

Страница 28: ...hermostatted column compartment Figure 9 Where to go from the System Screen Menu Structure Auto On On Error Printer Setup Display Settings User Settings Interfaces Column Identific Reset Balance Home Pressure Test Leak Test Temperature Calibration Change Arm Wavelength Calibr DAC Test System Log Error Log EMF Data Maintenance log Firmware Update Identify Print System Control Configure Tests Record...

Страница 29: ...ector wavelength elapsed time etc depending on the LC system configuration The screen content is updated in short intervals of roughly one second You can also monitor the LC system and module status using the Status bar at the top of each screen The content of the Status bar varies depending on the screen and is very limited The status for each module can also be displayed In the respective Settin...

Страница 30: ...ou can specify the vials you want to analyze and the desired method A graphical image of the tray offers a good view of the vials chosen The Sequence option gives you control over the execution of specified analysis sequence steps programmed before While the analysis is running you can view the processed samples on the tray image Figure 11 Samples Screen NOTE This screen is only available if an au...

Страница 31: ...ignal Directly from the Plot screen you can use a cursor to pinpoint information and you can observe information like run time status pressure ripple or composition Using the Direction and Selection keys of the control module you can change the scale of the plot window With the Rescale button F7 you can optimize the screen according to the selected signal and parameter ranges Figure 12 Plot Screen...

Страница 32: ...16B These require the Agilent 1200 Instant Pilot G4208A as controller or the Agilent ChemStation B 02 01 Table 1 Control Module Versions Version controls G1323B Firmware B 04 02 1100 1200 module firmware revision A 06 xx B 01 xx and above Agilent 1100 1200 Isocratic G1310A Quaternary G1311A and Binary G1312A pump Agilent 1100 1200 Autosampler G1313A and Thermostatted Autosampler G1330A Agilent 110...

Страница 33: ...lector G1364 Agilent 1100 Refractive Index Detector G1362A Agilent 1100 Multiple Wavelength Detector G1365A B Agilent 1100 Well plate Autosampler G1367A Agilent 1100 Capillary Pump G1376A Agilent 1100 Micro Well plate Autosampler G1377A G1368A Agilent 1100 Dual loop Autosampler G2258A Agilent 1100 Preparative Autosampler G2260A G2261A G1323A Firmware A 02 10 1100 module firmware revision up to A 0...

Страница 34: ...34 1200 Series Handheld Control Module Users s Guide 1 The Agilent 1200 Series Control Module Control Module Versions ...

Страница 35: ...ling the Control Module 36 General Functions 37 Troubleshooting 39 Inserting and Removing PC cards 40 Working with Methods 41 Time Programming 47 Automating Analyses 48 Displaying Data Graphically 50 Logbooks 53 Printing Screens 55 Connecting External Devices 57 Firmware Updates 60 Coexecution with Agilent ChemStation 61 ...

Страница 36: ...ler area network connector of the control module cable to one of the two CAN connectors on one of the Agilent 1200 Series modules WARNING The CAN connectors are similar to LAN adapter connectors Do not insert LAN connectors into the CAN or vice versa since the CAN uses 24 V and might blow up the LAN card Figure 13 Connecting the CAN Connector to Rear Panel CAN connector ...

Страница 37: ...atively choose Configure and select LC System Press Date Time F4 When date and time are changed in the control module the settings are automatically stored in all the connected modules The date and time are backed up by a battery in each module The control module does not have a clock of its own Setting Display Contrast From the System screen choose Configure and select LC System Press Display and...

Страница 38: ...ted Modules to the Available Modules list boxes and vice versa depending on which module is highlighted Configuring the LC System The LC system is self configuring to a large extent It recognizes automatically which modules are installed The layout of the Analysis screen changes according to the modules present You can use the Configure button F2 in the System screen to configure various features ...

Страница 39: ...errors occurred The logbook is self updating where the newest entry replaces the oldest entry If a printer is connected you can easily print out the logbook Troubleshooting the Control Module If your control module does not work correctly disconnect the module CAN connector from the rear of the Agilent 1200 Series module it is attached to and reconnect it If the problem still remains then power do...

Страница 40: ...ple screen press the m key and select Restart to restart the control module 3 To remove the PC card press the card eject button on the left hand side of the module NOTE The control module supports PCMCIA 2 0 type I and II memory cards In general only cards up to a capacity of 128 MB are supported Compatible cards are SanDisk CompactFlash Memory Cards SDCFBx yy with SanDisk CompactFlash PC Card Ada...

Страница 41: ...ansferred to another LC system Methods cannot be run directly from the PC card The method must first be loaded from the PC card before it can be run When the PC card method is loaded it becomes a module method Unless stated otherwise the following sections refer to module methods Loading a Method A method can be loaded using the Method button F3 in the Analysis or Samples screen 1 Enter the Analys...

Страница 42: ... eject speed can be modified using the Settings button F1 This button displays the Settings menu from which all LC system and module settings can be accessed If you change a method setting the value is immediately downloaded to the LC module An asterisk will appear in the right corner of the status line to indicate the current method has been modified The time programmable settings can be modified...

Страница 43: ...n F8 4 Select the Protected check box 5 Select the Done button F6 The method is now protected against inadvertent changes Any changes to the method will not be accepted until the method is unprotected by saving it again without protection For further protection remove the Control Module from the Instrument and store it in a secure place Any unauthorized method or instrument changes can be traced b...

Страница 44: ...t the Method button F3 in the Analysis screen 2 Select Save As using the Action keys 3 Enter a name as described in Specifying a Method Name on page 42 or simply continue with the next step if you want to keep the current method name 4 Press Enter 5 Select the Done button F6 to store the method in the modules Select Yes to confirm save in case you are overwriting an existing method The stored meth...

Страница 45: ...and from PC card 1 Insert the PC card into the control module as described in Inserting and Removing PC cards on page 40 2 Select the Method button F3 in the Analysis or Samples screen 3 Select the PC card button F2 4 Use the Selection and Direction keys to navigate within and between the method list boxes 5 Use the Copy and Copy buttons F7 F8 to copy methods from and to the PC card NOTE Methods f...

Страница 46: ...can be transferred from one LC system to another using a PC card 1 Save the method you want to transfer onto a PC card See section Exchanging Methods With the PC Card on page 45 2 Remove the PC card from the control module 3 Insert the PC card into the control module of the other LC system 4 Restart the control module using the Restart function via the m menu key available from the main screens 5 ...

Страница 47: ... and linear manner The timetable is accessed by selecting the Timetable button F2 in the Analysis screen A timetable line can be inserted by pressing the Insert button F7 and consists of the following Time Set the time span between the instant of injection and the desired parameter change Module Choose the module that controls the parameter you want to change Setting Select the parameter to be cha...

Страница 48: ...through the Logbook button F5 in the Sequence screen At the end of the sequence you can specify either to load a method e g to flush the LC system to remove buffer salts to avoid crystallization or to program a soft shut down method or to turn off the LC system using the End Actions button F1 If both options are selected the shut down method will be loaded to be available for the next user However...

Страница 49: ...he relevant parameters as well as timetable or injector program settings Wait Time Specify a wait time that creates a gap between method loading and execution This allows certain module parameters to stabilize before the next analysis is performed Calibration Settings For each sequence line you can define calibration settings by pressing the Calibration button F1 and choosing Edit Delete from the ...

Страница 50: ... the Direction and Selection keys to navigate within and between the Available Signals and Selected Signals list boxes 3 Exchange signals between the list boxes by pressing the Move button F8 or the Enter key On the right hand side from the Selected Signals list box you can see the legend to the signals You can also enter a time range X axis for the plot in this screen The different signals can be...

Страница 51: ...l unit axis there are several possibilities From the Plot Selection windows available from the Plot screen via the Select button F6 choose a signal from either list box and press the Setup button F7 You can specify a Y range separately for each signal This setting can also be made for signals not being part of the Selected Signals list box Rescaling directly from the Plot screen will overwrite the...

Страница 52: ...an be toggled by pressing the 1 2 3 keys on the numeric keypad Displaying Exact Signal Values Selecting the Cursor button F8 in the Plot screen displays the X and Y value of the current cursor position for the active signal In this mode using the Selection keys you can rescale the Y axis Using the Direction left right keys you can move the cursor along the graph in an X direction in order to find ...

Страница 53: ...y in the System screen and choosing the Setup Logbook option In the appearing logbook settings menu you can choose which events are to be displayed in the logbooks Choose a module from the list box and press the button F1 F4 corresponding to the type of logbook you want to open Maintenance Logbook On the screen you see the most recent maintenance work together with date and time Use the Add button...

Страница 54: ... messages are stored until the module is restarted or turned off EMF Early Maintenance Feedback In the Records screen the EMF indicator shows if a regular maintenance is needed The EMF limits can be scheduled by pressing the EMF button F1 in the Records screen and choosing Setup Limits Depending on the highlighted module you enter a window where the maintenance intervals can be set Consult the onl...

Страница 55: ...can be made individually for each module in the respective configuration settings see Connecting External Devices on page 57 If the setup is complete you may print a test page using the corresponding button Print Plot In the Plot screen you can create a screen hard copy using the m menu key and selecting Print Plot from the context menu The printout will also include a legend and the date and time...

Страница 56: ...d firmware version numbers together with the operation time This button is also available from the System Log Error Log Main Log and EMF windows Print Method The current method settings can be printed out using the Print button F6 in the Method screen The print dialog as described above will appear This will print all method settings excluding vial range information Print Timetable Pressing the m ...

Страница 57: ...rfaces see the corresponding sections in the modules reference manuals APG Remote Via the 9 pin APG remote connector included in all modules the system can communicate with external devices in order to synchronize the analyses This is necessary when an external device needs some time in order to get ready for a new analysis and thus transmitting of a start request is required see also Synchronizin...

Страница 58: ...est Prepare This causes the modules to get ready for the next analysis e g the detector will perform a balance Start Request This signal causes the modules to get ready for the analysis e q the autosampler will begin the injection cycle As soon as all conditions to start the analysis the injection needle is placed in the seat and the valve is in the proper position are fulfilled a Start signal is ...

Страница 59: ...onnect a printer to the serial interface included in all modules The module communication is enabled with the CAN cables Choose one module to connect the printer to MIO This interface enables the Agilent 1200 Series modules to communicate with PCs configured as Agilent ChemStations using a local area network LAN You can use the MIO interface if the respective extension board is installed in one of...

Страница 60: ...at holds the firmware files The installation of older firmware might be necessary to keep all systems on the same validated revision or if third party control software requires a special version To upgrade downgrade the firmware 1 Download the firmware and the documentation from the Agilent web http www chem agilent com scripts cag_firmware asp 2 Load the firmware into the module s as described in...

Страница 61: ... The Agilent ChemStation can generate data files from a control module method or sequence In this case the pre fix and file name counter in the Single Sample Info section of the Agilent ChemStation must be enabled protocol mode only If the control module starts an analysis the Agilent ChemStation is the slave monitor system In general however it is not recommended to run both the Agilent ChemStati...

Страница 62: ...ion than the method on the control module e g additional DAD parameters that are only accessible from the Agilent ChemStation To have a method available on both controllers proceed as follows If the method is on the Agilent ChemStation and should be saved on the control module or PC card load the method on Agilent ChemStation and then save the method on the control module or PC card with Method Sa...

Страница 63: ...mp Turning the Pump On and Off 64 Entering Settings 64 Purging the Pump 64 Purging Procedure 65 Adjusting Compressibility 66 Adjusting Stroke Volume 68 Automatic Shut down 68 Troubleshooting the Pump 68 Tracking Pump History Pump EMF Limits 69 Resetting the Pump 70 Resetting the Pump Settings 70 ...

Страница 64: ...his function from the Settings button F1 available in the Analysis screen or the Control button F1 in the System screen Entering Settings All pump settings can be entered in the Pump Settings screen You can access this screen by selecting the Settings button F1 in the Analysis screen and then choosing the pump Commonly used settings such as flow and solvent composition can be set directly in the A...

Страница 65: ...ntinuous stream of solvent comes out of the outlet tube from the purge valve 8 Turn off the pump using the On Off button F7 in the Analysis screen and then close the purge valve 9 Repeat the procedure for the other channels you need to purge NOTE If a flow rate of more than 5 ml min is used on a quaternary pump redefine the upper pressure limit to 200 bar Accessible from the Analysis screen press ...

Страница 66: ...in a uniform and accurate flow regardless of the system pressure The compressibility setting can be accessed by using the Pump Settings button F1 in the Analysis screen Table 3 lists compressibility values for common solvents used in LC Table 3 Compressibility Values for Common LC Solvents Solvent Compressibility 10 6 per bar Acetone 126 Acetonitrile 115 Benzene 95 Carbon tetrachloride 110 Chlorof...

Страница 67: ...on for the compressibility of the mobile phase For each particular compressibility value the piston stroke distance piston moves and the speed at which the piston moves are adjusted accordingly compensating for the solvent compressibility For a mixture of solvents we recommend that you choose the compressibility value of the solvent that is present in the highest amount ...

Страница 68: ...he pressure pulsation is directly proportional to the stroke volume Decreasing the stroke volume decreases the pulsation amplitude giving a better signal to noise ratio with flow sensitive detectors To maintain the flow the pumping frequency is increased increasing the frequency of the pressure pulsation Increasing the pump frequency gives better peak area reproducibility at low flow rates Automat...

Страница 69: ...set up a preventive maintenance schedule for the pump For example make a note of the total number of liters pumped when you change the piston seals This volume is a benchmark reflecting the lifetime of the seals for your solvents and application When the limit is exceeded an early maintenance feedback message will appear indicating it is time to change the seals Changing the seals in advance will ...

Страница 70: ...em screen 2 Select Pump from the menu 3 Press Enter 4 Select the Reset button F7 to stop the pump operation and perform a hardware initialization Resetting the Pump Settings 1 Select the Settings button F1 in the Analysis screen 2 Select Pump from the menu 3 Press Enter 4 Select the Default button F7 to reset the pump settings to their default values ...

Страница 71: ...71 Agilent 1200 Series Handheld Control Module User s Guide Agilent Technologies 4 Using the Degasser Starting the Degasser 72 Removing Gas Bubbles 72 Changing Solvents 73 ...

Страница 72: ...s there is insufficient vacuum in the online degasser and the vacuum pump is on to create sufficient vacuum Removing Gas Bubbles If you see gas bubbles in the solvent tubing or inside the solvent filter 1 Disconnect the solvent tube of the first solvent channel from your pump 2 Connect the syringe adapter onto the syringe from degasser accessory kit 3 Pull the syringe plunger to draw solvent throu...

Страница 73: ...ing to a solvent that is immiscible with the solvent currently in the tubing 1 Replace the current solvent with iso propanol if current solvent is organic or with water if current solvent is an organic buffer or salt 2 Flush the online degasser and all tubing thoroughly 3 Replace the iso propanol or water with the new solvent ...

Страница 74: ...74 1200 Series Handheld Control Module Users s Guide 4 Using the Degasser Changing Solvents ...

Страница 75: ...ser s Guide Agilent Technologies 5 Using the Autosampler Configuring the Autosampler 76 Entering Settings 77 Aligning Transport Arm Gripper 80 Tracking Autosampler History EMF limits 81 Resetting the Autosampler 81 Troubleshooting the Autosampler 82 ...

Страница 76: ...r from the menu 3 Press Enter You can configure the syringe volume seat capillary volume action on missing vial trays interfaces etc Configuring Interfaces 1 Select the Configure button F2 in the System screen 2 Select Autosampler from the menu 3 Press Enter 4 Select the Interfaces button F1 5 Select the interfaces you want to operate see Connecting External Devices on page 57 6 Select the Done bu...

Страница 77: ... can be set directly in the Analysis screen More specific settings draw speed eject speed draw position offset can be entered using the More button F1 and choosing the Settings option Setting the Injection Mode There are two modes which can be set Standard without pre defined needle wash with pre defined needle wash wash vial number can be set If the pre defined needle wash function is enabled the...

Страница 78: ...apsed runtime The injection cycle will not be started This procedure reduces the total injection time without interfering with the current run By choosing the Overlap Injection Cycle option the next vial will be placed in the injection port after the selected elapsed runtime The following restrictions apply when using the Overlap option The elapsed runtime entry before the sample is processed must...

Страница 79: ...r This setting is available in the Analysis screen too Activate the check box to have the temperature controlled as soon as the cooled autosampler is turned on Using the More option in the Settings screen and selecting Signals you can choose among several thermostat parameters ambient temperature heat sink heat sink fan etc that will be referred to as Autosampler Auxiliary in the Plot Signals scre...

Страница 80: ...he desired values in the corresponding fields The field X Correction sets the default value for the axis parallel to the front panel left right motion Theta Correction describes the default angle of the transport arm towards the front back axis of the module rotation For detailed information on the axes consult your Autosampler reference manual 6 Select the Done button F6 to accept settings The ne...

Страница 81: ...ycles A limit can be specified for each function which you can use to schedule preventive maintenance Resetting the Autosampler 1 Select the Control button F1 in the System screen 2 Select Autosampler from the menu 3 Press Enter 4 Select the Reset button F7 to reset the autosampler hardware This resets the injection valve metering device and sampling unit Reset to Default Settings 1 Select the Set...

Страница 82: ...e individual parts of the autosampler The injector step functions can be used in troubleshooting to check the single steps of the injection cycle For a complete description of each step see the Reference Manual for the Agilent 1200 Series autosampler If you have a thermostatted autosampler you can also test the auxiliary signals by pressing the Signal button in the Test screen The auxiliary signal...

Страница 83: ...logies 6 Using the Manual Injection Valve About the Injection Valve 84 Making An Injection 85 Completely Filling the Sample Loop 85 Partially Filling the Sample Loop 86 How Much Sample Is Actually Injected 86 How Much Sample Do I Need 87 An Alternative Way to Fill The Loop 87 ...

Страница 84: ... two positions In the LOAD position the mobile phase is led from the pump directly to the column allowing you to inject your sample into the sample loop In the INJECT position the mobile phase is led through the sample loop flushing the contents of the loop onto the column Valve Positions Figure 15 Valve Positions 1 to 6 valve ports 7 inlet from pump 8 outlet to column 9 needle port 10 vents 11 sa...

Страница 85: ...ely Filling the Sample Loop This is the conventional method in which an excess of sample is used to fill the sample loop completely The volume of the loop determines the injection volume 1 Fill syringe with sample 2 Move valve to LOAD position 3 Insert needle of syringe into needle port until needle touches stator face Do not press too hard 4 Slowly inject sample 5 Leave syringe in position and mo...

Страница 86: ...needle port until needle touches stator face Do not press too hard 5 Slowly inject sample 6 Leave syringe in position and move valve to INJECT position 7 Remove syringe How Much Sample Is Actually Injected When the sample loop is completely filled the amount of sample injected is equal to the volume of the sample loop plus the volume of the valve passages 1 in rotor and 2 in stator This means that...

Страница 87: ...ent already in the loop and some of the sample could be lost through port 6 if you try to inject too much An Alternative Way to Fill The Loop When the sample loop is completely filled you can either inject the sample into the loop or use the syringe to draw the sample through the loop 1 Move valve to LOAD position 2 Place vent tube from port 6 into sample vial 3 Insert needle of syringe into needl...

Страница 88: ...88 1200 Series Handheld Control Module Users s Guide 6 Using the Manual Injection Valve An Alternative Way to Fill The Loop ...

Страница 89: ...sing the Detectors Turning the Lamp On and Off 90 Entering Settings 91 Resetting the Baseline 93 Configuring the Detector 94 Troubleshooting the Detector 95 Tracking Detector History 96 Resetting the Detector 97 Lamp Ignition Routine VWD only 98 Configuring the Analog Channel Output 99 ...

Страница 90: ...in the Analysis screen 1 Enter the Analysis screen 2 Select the On Off button F7 3 Select the Lamp button F6 The same can be achieved by using the On Off button F8 in the Detector Settings or Control screen available through the Analysis or System screens NOTE When the lamp is turned on a lamp ignition routine is started For more information Lamp Ignition Routine VWD only on page 98 ...

Страница 91: ...pectrum settings range threshold are accessed by selecting the More button F1 under DA Detector Settings Variable Wavelength Detector Settings for this detector include the wavelength peakwidth and signal polarity Pressing the More button F1 and choosing Settings you can enter autobalance settings and the margin for negative absorbance The option Spectrum allows you to enter a wavelength range for...

Страница 92: ...l temperature in the Analysis screen Temperature Peakwidth polarity and automatic recycling can be set in the RI detector settings screen This screen is accessed by selecting the Settings button F1 in the Analysis screen Automatic zero and automatic purge can be set by selecting the More button F1 under RI Settings NOTE Only the G1323B version of the control module allows control of the Multiple W...

Страница 93: ...using the Refractive Index Detector in the Detector Control screen Please note that this procedure is not available for the Fluorescence Light Detector 1 Select the Control button F1 in the System screen 2 Select the Detector from the menu 3 Press Enter 4 Select the Balance button F1 You can achieve the same using the m menu key in the Detector Settings window available from the Analysis screen ...

Страница 94: ...ng the Detectors Configuring the Detector Configuring the Detector 1 Select the Configure button F2 in the System screen 2 Select the Detector from the list 3 Press Enter You can now configure the detector analog output s lamp on at power on and the interfaces ...

Страница 95: ...tor 1 Select the Tests button F3 in the System screen 2 Select the Detector from the menu 3 Press Enter 4 Select the test you require The selection of tests depends on the For information about each test see the Reference Manual for the Agilent 1200 detectors NOTE The full test capability is only available from the LC ChemStation ...

Страница 96: ...or History 1 Select the Records button F4 in the System screen 2 Select the Detector from the menu 3 Press Enter to display the logbook You can check change and reset the lamp burn time and number of ignitions For the FL detector the flash lamp lifetime and for the RI detector the time since last purge can be checked ...

Страница 97: ...ing the Detectors 7 Resetting the Detector Resetting the Detector 1 Select the Settings button F1 in the Analysis screen 2 Select the Detector from the menu 3 Press Enter 4 Select the Default button F7 to reset the detector settings to their default values ...

Страница 98: ...position is re initialized Check of wavelength setting at 656 nm emission must be within 1 nm at reference side only to have no influence from flow cell condition If not correct a message for calibration is displayed Perform a wavelength calibration available within VWD Tests Check of intensity at 250 nm without cut off filter inserted at 250 nm with cut off filter inserted If the intensity check ...

Страница 99: ...x Detector have one at the back side of the module To configure the analog outputs 1 Press the Settings button F1 in the Analysis screen 2 Choose the detector and press enter 3 Press the Analog button F3 4 Either select the signal you want to monitor from the signal source drop down list Or if you have a variable wavelength detector fluorescence light detector choose the signal s from the list s t...

Страница 100: ...100 1200 Series Handheld Control Module Users s Guide 7 Using the Detectors Configuring the Analog Channel Output ...

Страница 101: ...nd Off 102 Entering Settings 103 Configuring the Column Compartment 104 Configuring the Column ID Module 105 Selecting Separated or Combined Mode 106 Selecting the Column Switching Valve optional 107 Troubleshooting the Column Compartment 108 Tracking Column Compartment History 109 Tracking Column ID History 110 Resetting the Column Compartment 111 ...

Страница 102: ...urning the Column Compartment On and Off You can turn the column compartment on and off by using the On Off button F7 in the Analysis screen 1 Enter the Analysis screen 2 Select the On Off button F7 3 Select the Temp button F7 The same can be achieved through the Settings or Control buttons F1 in the Analysis or Systems screens ...

Страница 103: ...perature can be entered in the Analysis screen The temperature for left and or right heater can be entered in the Column Compartment Settings screen This screen is accessed by selecting the Settings button F1 in the Analysis screen Additional settings are accessed by selecting the More button F1 under Column Compartment Settings ...

Страница 104: ...guring the Column Compartment Configuring the Column Compartment 1 Select the Configure button F2 in the System screen 2 Select Column Comp from the list 3 PressEnter You can now configure the leak detection mode temperature on at power on the interfaces and the column identification module ...

Страница 105: ...Enter your column data into the fields as required Additional fields are available by pressing the More button F1 7 Pressing the Write button F7 will transfer the information into the column tag 8 Pressing the Right button F8 will show the information fields of the right column tag if column is installed NOTE If no column tag is sensed the record fields are not active and the left right tag sign i...

Страница 106: ...ned Mode This mode allows the temperature setting of both heaters independent from each other If not enabled both heaters are kept on the same temperature 1 Select the Settings button F1 in the Analysis screen 2 Select Column Comp from the menu 3 Enable the Separated mode by selecting the check box 4 To enable the Combined mode deselect the check box ...

Страница 107: ...mn Switching Valve optional Selecting the Column Switching Valve optional The column switching valve is optional 1 Select the Settings button F1 in the Analysis screen 2 Select Column Comp from the menu 3 Press the Column Switch button 4 Select mode 1 or 2 5 Press the Done button F6 and leave this screen ...

Страница 108: ...g the Column Compartment 1 Select the Tests button F3 in the System screen 2 Select Column Comp from the menu 3 Press Enter 4 Select the test you require You can select the Calibrate test to check the operation of the Column Compartment For information about the test see the Reference Manual for the Agilent 1200 Series column compartment ...

Страница 109: ...9 Using the Column Compartment 8 Tracking Column Compartment History Tracking Column Compartment History 1 Select the Records button F2 in the System screen 2 Select Col Comp from the list 3 Press the System or Maint Log button F4 to display the logbook ...

Страница 110: ...tion screen 4 Press the Column ID button F2 to enter the Column ID screen By selecting the More button F1 you can enter values and check a variety of features for the installed column For example the maximum pressure allowed and the maximum recommended temperature You can check the number of injections that have been made on the column NOTE If no column tag is sensed the parameter entry fields are...

Страница 111: ...n Compartment 8 Resetting the Column Compartment Resetting the Column Compartment 1 Select the Settings button F1 in the Analysis screen 2 Select Column Comp from the menu 3 PressEnter 4 Select the Default button F7 to reset the detector settings to their default values ...

Страница 112: ...112 1200 Series Handheld Control Module Users s Guide 8 Using the Column Compartment Resetting the Column Compartment ...

Страница 113: ...rol Module User s Guide Agilent Technologies 9 Running an Isocratic Analysis What You Will Need 114 Preparing the LC System 115 Entering Settings 116 Saving Settings in a Method 118 Observing the Chromatogram 119 Starting the Analysis 121 ...

Страница 114: ...r Column A 125 mm 4 0 mm Hypersil ODS 5 µm Agilent Technologies part number 7982618 564 Solvents For the isocratic pump a solvent mixture of LC grade bidistilled water 35 and acetonitrile 65 Sample The Agilent Technologies isocratic standard sample Agilent Technologies part number 01080 68704 This contains 0 15 wt dimethylphthalate 0 15 wt diethylphthalate 0 01 wt biphenyl and 0 03 wt o terphenyl ...

Страница 115: ...r lamp and pump using the On Off button F7 in the Analysis screen Use Action keys then to select the module 3 For the quaternary pump turn on the degasser by pressing the line power switch 4 Purge the pump For more information see Chapter 3 Using the Pump 5 Allow the detector at least 15 minutes to provide a stable baseline 6 Fill the contents of a Agilent Technologies isocratic standard sample am...

Страница 116: ...t the Settings button F1 6 Select LC System from the menu 7 Select the Default button F7 and select the Yes button to load defaults 8 Press Esc 9 Select the Settings button F1 10 Select the pump from the menu either ISO BIN or QUAT 11 Press the Pressure button F3 12 Enter 400 as the upper pressure limit 13 Select the Done button F6 14 Enter the following values B 65 C OFF D OFF for quarternary pum...

Страница 117: ... Guide 117 Running an Isocratic Analysis 9 Entering Settings NOTE The channels of a pumping system are named A B C and D depending on the pump type A is automatically calculated by 100 B C D If no values for B C and D are entered A is always 100 ...

Страница 118: ...d Saving Settings in a Method 1 Select the Method button F3 in the Analysis screen 2 Select the Save As button F8 3 Enter the method name as ISO using the selection keys also see Specifying a Method Name on page 42 4 Press the Done button F6 to save the method 5 Press the Esc key to return to the Analysis screen ...

Страница 119: ...Select the Done button F6 to display the chromatogram 6 Press the Esc key to go back to the Analysis screen 7 Press the Start button F8 8 Select OK to confirm the vial range and injection number and press Enter to start the analysis NOTE You can choose several signals at a time The plot function will display all signals that are shown in the Selected Signals list box Use the selection keys to navi...

Страница 120: ...mn you have used Differences in retention times and areas of the peaks in your chromatogram and the one shown in Figure 16 might be a result of variations in the concentration of the sample from batch to batch the quality of the solvents used and the column temperature Figure 16 Analysis of Isocratic Standard Sample min 1 2 3 4 5 6 mAU 0 50 100 150 200 250 300 350 NOTE You can rescale the plot usi...

Страница 121: ...e Analysis Starting the Analysis 1 Enter the Analysis screen 2 Select the Start button F8 3 Select OK to confirm the vial range and injection number and press the Enter key to start the analysis NOTE The analysis can be started from various other screens e g System Status Sampels or Sequence screen ...

Страница 122: ...122 1200 Series Handheld Control Module Users s Guide 9 Running an Isocratic Analysis Starting the Analysis ...

Страница 123: ...trol Module User s Guide Agilent Technologies 10 Running a Gradient Analysis What You Will Need 124 Preparing the LC System 125 Entering Settings 126 Saving Settings in a Method 128 Observing the Chromatogram 129 Starting the Analysis 131 ...

Страница 124: ...UV detector and degasser Column A 125 mm 4 0 mm Hypersil ODS 5 µm Agilent Technologies part number 7982618 564 Solvents LC grade bidistilled water and acetonitrile Sample The Agilent Technologies isocratic standard sample Agilent Technologies part number 01080 68704 This contains 0 15 wt dimethylphthalate 0 15 wt diethylphthalate 0 01 wt biphenyl and 0 03 wt o terphenyl dissolved in methanol ...

Страница 125: ...sis screen 3 For the quaternary pump turn on the degasser by pressing the line power switch 4 Purge the pump For more information see Chapter 3 Using the Pump 5 Allow the detector at least 15 minutes to provide a stable baseline 6 Fill the contents of a Agilent Technologies isocratic standard sample ampoule into a vial and seal the vial with a cap Place the vial in position 1 of the autosampler tr...

Страница 126: ...reen The example assumes that the Timetable is empty If the Timetable is not empty use the All Lines item accessed by pressing the Delete button 1 Enter the Analysis screen 2 Set the Vial Range as 1 to 1 3 Set the Number of Injections to 1 4 Skip the method name field 5 Select the Settings button F1 6 Select LC System from the menu 7 Press Enter 8 Select the Default button F7 and select the Yes bu...

Страница 127: ...mposition to 65 C and D to 0 00 If you have a binary pump set the B composition to 65 19 Select the Enter button to accept entries Action Key F7 20 Enter the following information for another two lines time 4 B 95 time 5 B 65 21 Press the Done button and verify your timetable entries 22 Press the Esc key until you are in the Analysis screen NOTE The channels of a pumping system are named A B C and...

Страница 128: ...thod Saving Settings in a Method 1 Select the Method button F3 in the Analysis screen 2 Select the Save As button F8 3 Enter the method name as GRAD using the selection keys also see Specifying a Method Name on page 42 4 Press the Done button F6 to save the method 5 Press ESC to return to the Analysis screen ...

Страница 129: ... 5 Select the Done button F6 to display the chromatogram 6 Press Esc to go back to the Analysis screen 7 Press the Start button F8 8 Select OK to confirm the vial range and injection number and press Enter to start the analysis NOTE You can choose several signals at a time The plot function will display all signals that are shown in the Selected Signals list box Use the selection keys to navigate ...

Страница 130: ...nd the one shown in Figure 17 might be a result of variations in the concentration of the sample from batch to batch the quality of the solvents used and the column temperature If you compare this chromatogram with the one from Chapter 9 Running an Isocratic Analysis you will notice the solvent gradient has reduced the elution time of the fourth peak in the chromatogram Figure 17 Gradient Analysis...

Страница 131: ...is Starting the Analysis 1 Enter the Analysis screen 2 Select the Start button F8 3 Select OK to confirm the vial range and injection number and press Enter to start the analysis NOTE The analysis can be started by pressing the Start button F8 from the Samples screen the System screen or the Status screen ...

Страница 132: ...132 1200 Series Handheld Control Module Users s Guide 10 Running a Gradient Analysis Starting the Analysis ...

Страница 133: ... Multiple Vials Using the Same Method 134 Analyzing Multiple Vials Using Different Methods 135 Single Level Calibration Sequences 137 Multiple Level Calibration Sequences 139 Recalibrating With the Same Group of Standards 139 Recalibrating With Multiple Groups of Standards 141 Synchronizing Analyses with External Devices 144 ...

Страница 134: ...on from each vial You will use a previously created method The samples are located in positions 1 to 25 of the autosampler tray For details Working with Methods on page 41 1 Enter the Analysis screen 2 Set the Vial Range from 1 to 25 3 Set the number of injections to 1 4 Select the method you want to use with the scroll buttons 5 Select the Start button F8 6 Select OK to confirm the vial range and...

Страница 135: ...ls are analyzed using METH1 with one injection per vial the next 20 vials are analyzed using METH2 with two injections per vial the last 10 vials are analyzed using METH3 with three injections per vial The vials are located in positions 1 to 50 of the autosampler tray The example assumes that the sequence table is empty If the sequence table is not empty use the All Lines item accessed by pressing...

Страница 136: ...ods 5 Select the Enter button to accept entries 6 For sequence line 3 enter 7 Select the Done button F6 to enter sequence line 3 settings and complete the sequence 8 Select the Start button F8 in the Sequence screen 9 Select the OK button to start the sequence Vial Range 41 to 50 Inj 3 Inj Volume Default Method METH3 Wait 30 minutes ...

Страница 137: ...e after every 2 samples C S S C S S C S S C S S C S C the calibration standard is located in position 90 and the 9 sample vials are in positions 1 to 9 of the autosampler tray and the method called METH1 is used for the samples and standards The example assumes that the sequence table is empty If the sequence table is not empty use the All Lines item accessed by pressing the Delete button F6 1 Sel...

Страница 138: ...ences 4 Enter the following information 5 Press the Done button F6 until the Sequence screen appears 6 Select the Start button F8 in the Sequence screen 7 Press Enter to start the sequence Calibration vial range 90 to 90 Number of injections 1 Calibration method METH1 Recalibrate every 2 vials multi Before On After On ...

Страница 139: ... once the calibration standards are analyzed twice before the samples and re analyzed twice after every 5 samples C1 C2 C3 S10 S14 C1 C2 C3 S15 S19 C1 C2 C3 S20 S24 C1 C2 C3 the calibration standards are located in positions 90 to 92 of the autosampler tray the 15 sample vials are located in positions 10 to 24 of the autosampler tray and the samples and standard are analyzed using a method called ...

Страница 140: ...ies 6 Select the Start button F8 in the Sequence screen 7 Press Enter start the sequence c The autosampler now analyzes the three calibration standards in duplicate sample vials 10 through 14 the three calibration standards in duplicate sample vials 15 through 19 the three calibration standards in duplicate sample vials 20 through 24 and the three calibration standards in duplicate Calibration via...

Страница 141: ... C3 S11 S12 C1 C2 C3 The calibration standards of type A are in positions 1 2 and 3 of the autosampler tray and the 6 sample vials are in positions 7 to 12 and the samples and the calibration standards use the same method called METH1 For sample type B there are 3 calibration standards of different concentrations and 9 samples each sample must be analyzed once the calibration standards must be ana...

Страница 142: ...the Calibration Settings screen for sequence line 1 and choose Edit 4 Enter the following information 5 Select the Done button F6 to accept entries 6 Move the highlighted bar to line 2 by pressing the Selection key down and press Enter 7 For sequence line 2 enter 8 Select the Calibration button F1 to display the Calibration Settings screen for sequence line 2 and choose Edit Vial Range 7 to 12 Inj...

Страница 143: ...dards in duplicate type A samples in vials 9 and 10 three type A calibration standards in duplicate type A samples in vials 11 to 12 three type A calibration standards in duplicate three type B calibration standards in duplicate type B samples in vials 13 14 and 15 three type B calibration standards in duplicate type B samples in vials 16 17 and 18 three type B calibration standards in duplicate t...

Страница 144: ...ll the analysis preparation using the control module Standard In the standard mode the analysis is under the command of the control module The control module will issue a Start Request command to the autosampler as soon as all modules are ready for the next analysis The autosampler issues the Start command at the point of injection With an Agilent 1200 Autosampler integrated in the system and no e...

Страница 145: ...ws to trigger the external device which will start each injection by sending a start signal The vial range or sequence will be started by the control module but for every new injection the external device must give the start command In this mode the control module will simply track the progress of the vial range or sequence It will indicate the proper sample change sequence lines load methods or o...

Страница 146: ...he Start button F8 has been pressed a Start Request is issued before each run and the external device must give the Start command for the injection process Here too the module will simply track the progress of the analysis Wait for Single External Start Request After pressing the Start button F8 the autosampler will wait for a single external start request on the APG remote lines When the start re...

Страница 147: ...o get ready for the next analysis and thus has to be in charge of the start event Figure 21 Wait for repeated external start request NOTE An Agilent 1200 variable wavelength detector or diode array detector will perform a balance provided that Auto Balance is set to Prerun in the More Settings screen when receiving a start command from the control module This will only happen in the Standard and W...

Страница 148: ...148 1200 Series Handheld Control Module Users s Guide 11 Running Multiple Vial Analyses Synchronizing Analyses with External Devices ...

Страница 149: ...9 Agilent 1200 Series Handheld Control Module User s Guide Agilent Technologies 12 Running an Injector Program Creating an Injector Program 150 Entering Injector Program Settings 151 Saving the Method 153 ...

Страница 150: ...set the LC system to the default settings modify the injector program settings in the Inj Program screen the other settings will remain with their default values and save the method with the name DERIV When the method called DERIV is started the program is executed followed by the analytical run The first step of the program involves drawing derivatization reagent into the needle capillary followe...

Страница 151: ...ad defaults 5 Press Esc to go back to the Analysis menu 6 Select the Settings button F1 7 Select Autosampler from the menu 8 Select the Inj Program button F3 to display the Program screen 9 For program line 1 select the Insert button F7 10 Select the DRAW function and enter the draw settings as described in Table 6 on page 152 11 Select the Enter button F7 to accept entries 12 Repeat the procedure...

Страница 152: ... needle capillary 3 WASH in vial 2 VIAL 1 time Cycles Wash the needle tip in the wash vial 2 4 DRAW 2 µl AMOUNT from sample SOURCE Add 2 µl of the sample in the autosampler tray to the derivatization volume already in the needle capillary 5 WASH in vial 2 VIAL 1 time CYCLE Wash the needle tip in the wash vial 2 6 DRAW 1 µl AMOUNT from vial 1 SOURCE Add 1 µl of derivatization reagent from vial 1 in...

Страница 153: ...een 2 Select the Save As button F8 3 Enter the method name as DERIV using the selection keys also see Specifying a Method Name on page 42 4 Press the Done button F6 to save the method 5 Press the Esc key to return to the Analysis screen The injector program is now part of the method called DERIV and can be started by selecting the Start button F8 in the Analysis screen ...

Страница 154: ...154 1200 Series Handheld Control Module Users s Guide 12 Running an Injector Program Saving the Method ...

Страница 155: ...155 Agilent 1200 Series Handheld Control Module User s Guide Agilent Technologies 13 Maintaining the Control Module Control Module Parts 156 This chapter shows the repair items ...

Страница 156: ...dule Parts The Controll Module s repair level is shown below Table 7 Control Module Parts Item Description Part Number Control Module replacement part including cable G1323 67011 Plastic Housing Kit includes front back and a clamp 5065 9984 CAN cable Agilent module to control module G1323 81600 Figure 22 Control Module ...

Страница 157: ...157 Agilent 1200 Series Handheld Control Module User s Guide Agilent Technologies A Appendix Safety Information 158 Radio Interference 161 Agilent Technologies on Internet 161 ...

Страница 158: ...fety standards Operation Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective ...

Страница 159: ... of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument ...

Страница 160: ... could cause physical injury or damage to the equipment Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A caution alerts you to situations that could cause a possible loss of data Do not proceed beyond a caution until you have fully understood and met the indicated conditions ...

Страница 161: ...Test and Measurement If test and measurement equipment is operated with equipment unscreened cables and or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at htt...

Страница 162: ...162 1200 Series Handheld Control Module Users s Guide A Appendix Agilent Technologies on Internet ...

Страница 163: ...ary signals 79 configuration 76 defaults 81 draw position offset 77 draw speed 77 eject speed 77 entering settings 77 external contacts 76 injection modes 77 injection volume 77 injector program 77 interfaces 76 missing vial 76 needle seat volume 76 needle wash 77 optimization 78 overlapped injections 78 prefetch 78 resetting 81 runtimes 77 sample throughput 78 shortening analysis time 78 syringe ...

Страница 164: ... history 109 tracking ID history 110 troubleshooting 108 using 101 column ID button 105 column ID module 105 column switch button 107 column switching valve 107 compressibility 66 configure 38 context menu 20 contrast 37 control module Agilent ChemStation 61 available modules 38 coexecution 61 contrast 37 display 37 displayed modules 38 features 16 firmware update 61 general functions 37 installin...

Страница 165: ...ions 37 GLP compliance 16 GPIB interface 57 gradient analysis 123 126 130 graphical display 50 graphs 50 gripper 80 H help 19 history autosampler 81 column compartment 109 detector 96 pump 69 I i info key 19 icons EMF 27 53 ID tag 105 identify button 53 identify modules 53 initialize 45 injection mode 77 injection valve manual 83 injection volume 77 injector program 77 149 155 creating 150 156 ent...

Страница 166: ...ecalibration muliple standards 141 multiple vial analysis 133 N navigation key 21 needle seat volume 76 needle wash 77 numeric keypad 22 numeric keys 22 O on error 38 on off 65 column compartment 102 degasser 72 diode array detector 90 fluorescence light detector 90 pump 64 variable wavelength detector 90 on off button 25 37 65 90 102 online help 19 online information system 19 operation time 53 o...

Страница 167: ... 44 saving method 118 128 screens analysis 23 24 25 config 55 method 56 plot 23 31 50 program 56 records 53 samples 23 30 sequence 48 status 23 29 system 23 27 timetable 42 views 24 search button 19 select button 50 select signals 50 selection keys 21 sequence 24 48 135 137 139 calibration 48 137 139 end actions 48 logbook 48 printing 56 recalibration 48 sequence button 48 sequence modes 144 seria...

Страница 168: ...p 68 turning on off column compartment 102 U units 38 updating firmware 60 user ID 44 V valve column compartment 107 filling sample loop 85 87 filling sample loop partially 86 making an injection 85 manual 83 opening 64 positions 84 sample 86 87 variable wavelength detector 89 analog output 94 balance 93 interfaces 94 lamp ignition routine 98 lamp on off 90 98 resetting 97 resetting the baseline 9...

Страница 169: ......

Страница 170: ...le and how to operate the Agilent 1200 Series modules and system for HPLC The Agilent 1200 Series Control Module Working with the Control Module Using the Pump Using the Degasser Using the Autosampler Using the Manual Injection Valve Using the Detectors Using the Column Compartment Running an Isocratic Analysis Running a Gradient Analysis Running Multiple Vial Analyses Running an Injector Program ...

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