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Optimizing Performance

Optimization for Lowest Carry-over

Optimization for Lowest Carry-over

Several parts of an injection system can contribute to carry-over:

•

needle outside

•

needle inside

•

needle seat

•

sample loop

•

seat capillary

•

injection valve

The autosampler continuous flow-through design ensures that sample loop,
needle inside, seat capillary, and the mainpass of the injection valve is always
in the flow line. These parts are continuously flushed during an isocratic and
also during a gradient analysis. The residual amount of sample remaining on
the outside of the needle after injection may contribute to carry-over in some
instances. When using small injection volumes or when injecting samples of
low concentration immediately after samples of high concentration,
carry-over may become noticeable. Using the automated needle wash
enables the carry-over to be minimized and prevents also contamination of
the needle seat.

Using the Automated Needle Wash

The automated needle wash can be programmed either as “injection with
needle wash” or the needle wash can be included into the injector program.
When the automated needle wash is used, the needle is moved into a wash
vial after the sample is drawn. By washing the needle after drawing a sample,
the sample is removed from the surface of the needle immediately.

Uncapped Wash Vial

For best results, the wash vial should contain solvent in which the sample
components are soluble, and the vial should not be capped. If the wash vial is
capped, small amounts of sample remain on the surface of the septum, which
may be carried on the needle to the next sample.

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Содержание G1313A

Страница 1: ...s1 Agilent 1100 Series Standard Micro and Preparative Autosamplers Reference Manual ...

Страница 2: ... warranty of any kind with regard to this material including but not limited to the implied warranties or merchantability and fitness for a particular purpose Agilent Technologies shall not be liable for errors contained herein or for incidental or consequential damages in connection with the furnishing performance or use of this material WARNING For details of safety see Safety Information on pag...

Страница 3: ...Reference Manual Agilent 1100 Series Standard Micro and Preparative Autosamplers ...

Страница 4: ...tive Autosamplers and their respective Thermostatted version The manual describes the following installing the autosampler optimizing performance troubleshooting and test functions repairing the autosampler parts and materials introduction to the autosampler theory of operation and control module screens ...

Страница 5: ... Flow Connections 31 Sample Trays 32 Choice of Vials and Caps 35 Transporting the Autosampler 38 2 Optimizing Performance How to optimize the autosamplers to achieve best results 39 Optimization for Lowest Carry over 41 Fast Injection Cycle and Low Delay Volume 44 Precise Injection Volume 46 Choice of Rotor Seal 48 3 Troubleshooting and Test Functions The modules built in troubleshooting and test ...

Страница 6: ...en Cover 64 Restart Without Cover 65 Arm Movement Failed 66 Valve to Bypass Failed 67 Valve to Mainpass Failed 68 Needle Up Failed 69 Needle Down Failed 70 Missing Vial 71 Initialization Failed 72 Metering Home Failed 73 Motor Temperature 74 Initialization with Vial 75 Safety Flap Missing 76 Vial in Gripper 77 Missing Wash Vial 78 Invalid Vial Position 79 Maintenance Functions 80 Change Needle 81 ...

Страница 7: ...oes through the gripper arm into the via 97 Poor alignment seen during vial pickup and vial replacement and or when the needle hits the gripper arm 99 4 Repairing the Autosampler Instructions on simple routine repair procedures as well as more extensive repairs requiring exchange of internal parts 101 Updating the Firmware 103 Cleaning the Autosampler 104 Using the ESD Strap 105 Overview of Main R...

Страница 8: ...ly 132 Sampling Unit 134 Injection Valve Assembly 140 Metering Drive Motor and Belt 142 Needle Drive Motor and Belt 144 Fan 146 ASM Board 148 SUD Board 152 Power Supply 154 Leak Sensor 157 5 Parts and Materials Detailed illustrations and lists for identification of parts and materials 159 Main Assemblies 160 Sampling Unit Assembly 162 Analytical Head Assembly 165 Injection Valve Assembly 168 Sheet...

Страница 9: ...ntenance Kit G1313 68709 181 Multi Draw Kit G1313 68711 182 900 µl Injection Upgrade Kit G1363A for G1313A 29A 183 External Tray G1313 60004 184 Cable Overview 185 Analog Cables 187 Remote Cables 189 BCD Cables 195 Auxiliary Cable 197 CAN Cable 197 External Contact Cable 198 RS 232 Cable Kit 199 LAN Cables 200 6 Introduction to the Autosampler An introduction to the standard micro and preparative ...

Страница 10: ...utosampler Main Board ASM 220 Firmware Description 226 Optional Interface Boards 228 Interfaces 231 Setting the 8 bit Configuration Switch 236 The Main Power Supply Assembly 241 8 Introduction to the Control Module Introduction to the screens available for operation of the Ag ilent 1100 Series autosamplers with the control module 245 Screens available from the Analysis screen 247 Screens available...

Страница 11: ... Micro and Preparative Autosamplers 269 Performance Specifications 270 10 Legal Safety and Warranty Information Warranty Statement 276 Safety Information 279 Lithium Batteries Information 282 Radio Interference 283 Sound Emission 283 Solvent Information 284 Agilent Technologies on Internet 285 ...

Страница 12: ...12 Contents ...

Страница 13: ...1 1 Installing the Autosampler Site requirements and installation of the autosampler and thermostatted autosampler ...

Страница 14: ... a power plug for the line connections The two modules are connected by a control cable and both are turned on by the autosampler module WARNING To disconnect the autosampler from line power unplug the power cord The power supply still uses some power even if the power switch on the front panel is turned off WARNING To disconnect the thermostatted autosampler from line power unplug the power cord ...

Страница 15: ... on either side and approximately 8 cm 3 1 inches at the rear for the circulation of air and room for electrical connections Ensure the autosampler is installed in a horizontal position The thermostatted autosampler dimensions and weight see allow the instrument to be placed on almost any laboratory bench The instrument requires an additional 25 cm 10 inches of space on either side for the circula...

Страница 16: ...than 50 C 122 F may cause the rear panel to become hot Table 1 Physical Specifications Autosampler G1313A G1329A G1389A G2260A 7 SH 6SHFLILFDWLRQ RPPHQWV Weight 14 2 kg 31 3 lbs Dimensions height width depth 200 345 435 mm 8 13 5 17 inches Line voltage 100 120 or 220 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption apparent power Power consumption active power 300 ...

Страница 17: ...een mechanically and electrically checked If the shipping container or cushioning material is damaged notify the carrier and save the shipping material for the carriers inspection CAUTION If there are signs of damage to the autosampler please do not attempt to install the autosampler Delivery Checklist Ensure all parts and materials have been delivered with the autosampler The instrument box conta...

Страница 18: ...00 pk 5182 0717 Label halftray 5989 3890 Vial instruction sheet no PN Wrenches 1 4 5 16 inch 8710 0510 Rheotool socket wrench 1 4 inch 8710 2391 Hex key 4 mm 15 cm long T handle 8710 2392 Hex key 9 64 mm 15 cm long T handle 8710 2394 Hex key 2 5 mm 15 cm long straight handle 8710 2412 ESD wrist strap 9300 1408 Finger caps x3 Reorder gives pack of 15 5063 6506 Tray for 40 x 2 ml vials G1313 44502 T...

Страница 19: ... instruction sheet no PN Wrenches 1 4 5 16 inch 8710 0510 Rheotool socket wrench 1 4 inch 8710 2391 Hex key 4 mm 15 cm long T handle 8710 2392 Hex key 9 64 mm 15 cm long T handle 8710 2394 Hex key 2 5 mm 15 cm long straight handle 8710 2412 ESD wrist strap 9300 1408 Finger caps x3 Reorder gives pack of 15 5063 6506 Front door cooled autosampler G1329 40301 Air channel adapter G1329 43200 Cover ins...

Страница 20: ...x Key 8710 0060 Wrench 4 mm both ends 8710 1534 Wrenches 1 4 5 16 inch 8710 0510 Rheotool socket wrench 1 4 inch 8710 2391 Hex key 4 mm 15 cm long T handle 8710 2392 Hex key 9 64 mm 15 cm long T handle 8710 2394 Hex key 2 5 mm 15 cm long straight handle 8710 2412 ESD wrist strap 9300 1408 Finger caps x3 Reorder gives pack of 15 5063 6506 Torque adapter G1315 45003 Front door cooled autosampler G13...

Страница 21: ...nches 1 4 5 16 inch 8710 0510 Rheotool socket wrench 1 4 inch 8710 2391 Hex key 4 mm 15 cm long T handle 8710 2392 Hex key 9 64 mm 15 cm long T handle 8710 2394 Hex key 2 5 mm 15 cm long straight handle 8710 2412 ESD wrist strap 9300 1408 Finger caps x3 reorder gives pack of 15 5063 6506 Front door cooled autosampler G1329 40301 Air channel adapter G1329 43200 Tray for 15 x 6 ml vials x2 G1313 445...

Страница 22: ...he position shown in Figure 1 and Figure 2 Figure 3 and Figure 4 show the configuration recommended for a thermostatted autosampler These configurations optimize the system flow path ensuring minimum delay volume Figure 1 Recommended Stack Configuration for an Autosampler Front View Solvent cabinet Vacuum degasser Pump Column compartment Autosampler or Micro Autosampler or Preparative Autosampler ...

Страница 23: ...r Optimizing the Stack Configuration Figure 2 Recommended Stack Configuration for an Autosampler Rear View GPIB or LAN to LC ChemStation Analog signal to recorder AC power Remote cable CAN Bus cable to control module CAN Bus cable ...

Страница 24: ...izing the Stack Configuration Figure 3 Recommended Stack Configuration for a Thermostatted ALS Front View Autosampler or Micro ALS or Preparative ALS Degasser ALS Thermostat Column Compartment Detector Control Module Pump Solvent Cabinet ...

Страница 25: ...Stack Configuration Figure 4 Recommended Stack Configuration for a Thermostatted ALS Rear View GPIB or LAN to LC ChemStation Analogsignalto recorder AC power Remote cable CAN bus cable Autosampler Thermostat cable AC power Analogsignalto recorder ...

Страница 26: ...l the LAN interface board in the sampler if required see Agilent 1100 Series Interface Board to general purposes on page 198 2 Remove the adhesive tape which covers the front door 3 Remove the front door and remove the transport protection foam 4 Place the Autosampler on the bench or in the stack as recommended in Recommended Stack Configuration for an Autosampler Front View on page 22 Preparation...

Страница 27: ...Turn ON power by pushing the button at the lower left hand side of the sampler Figure 6 Cable Connections NOTE If the front cover is not installed the autosampler is in a not ready condition and operation is inhibited NOTE The sampler is turned ON when the line power switch is pressed and the green indicator lamp is illuminated The detector is turned OFF when the line power switch is protruding an...

Страница 28: ... will then remain in the instrument This may damage the instruments electronics Figure 7 Condensation Leak outlet 3 Install the LAN interface board in the sampler if required see Agilent 1100 Series Interface Board to general purposes on page 198 4 Remove the adhesive tape which covers the front door 5 Remove the front door and remove the transport protection foam Preparation Locate bench space Pr...

Страница 29: ...the tray area of the Autosampler Figure 8 Air channel adapter 8 Re install the tray 9 Ensure the power switch on the front of the Autosampler is OFF and the power cables are disconnected 10 Connect the cable between the Autosampler and the Thermostat see Figure 9 WARNING Do not disconnect or reconnect the autosampler to ALS thermostat cable when the power cords are connected to either of the two m...

Страница 30: ...r switch is pressed and the green indicator lamp is illuminated The detector is turned OFF when the line power switch is protruding and the green light is OFF WARNING To disconnect the sampler from the line unplug the power cord The power supply still uses some power even if the power switch at the front of the panel is turned OFF WARNING To avoid personal injury keep fingers away from the needle ...

Страница 31: ...njection valve 2 Connect column compartment inlet capillary to port 6 of the injection valve 3 Connect the corrugated waste tube to the solvent waste from the leak plane 4 Ensure that the waste tube is positioned inside the leak channel NOTE Do not extend the waste capillary of the autosampler The siphoning effect might empty the complete seat capillary introducing air into the system Figure 10 Hy...

Страница 32: ...de the sample tray into the autosampler so that the rear of the sample tray is seated firmly against the rear of the sample tray area 4 Press the front of the sample tray down to secure the tray in the autosampler NOTE If the thermostatted autosampler tray pops out of position the air channel adapter is not correctly inserted Figure 11 Installing the Sample Tray ...

Страница 33: ...alf tray begin at position 101 as follows Left hand 40 position tray 1 40 Left hand 15 position tray 1 15 Right hand 40 position tray 101 140 Right hand 15 position tray 101 115 Table 6 Supported trays for the Autosampler G1313A HVFULSWLRQ 3DUW 1XPEHU Tray for 100 x 2 ml vials G1313 44500 Halftray for 15 x 6 ml vials G1313 44503 Halftray for 40 x 2 ml vials G1313 44502 Table 7 Supported tray for t...

Страница 34: ...34 Installing the Autosampler Sample Trays Figure 12 Numbering of Tray Positions Left hand half tray Right hand half tray Position 1 Position 101 ...

Страница 35: ...m of 15 000 injections with the Agilent 1100 Series autosampler Table 8 Crimp Top Vials Description Volume ml 100 Pack 1000 Pack 100 Pack silanized Clear glass 2 5181 3375 5183 4491 Clear glass write on spot 2 5182 0543 5183 4492 5183 4494 Amber glass write on spot 2 5182 3376 5183 4493 5183 4495 Polypropylene wide opening 1 5182 0567 5183 4496 Polypropylene wide opening 0 3 9301 0978 Table 9 Snap...

Страница 36: ...Description Septa 100 Pack Silver aluminum Clear PTFE red rubber 5181 1210 Silver aluminum Clear PTFE red rubber 5183 4498 1000 Pack Blue aluminum Clear PTFE red rubber 5181 1215 Green aluminum Clear PTFE red rubber 5181 1216 Red aluminum Clear PTFE red rubber 5181 1217 Table 12 Snap Caps Description Septa 100 Pack Clear polypropylene Clear PTFE red rubber 5182 0550 Blue polypropylene Clear PTFE r...

Страница 37: ...k Blue polypropylene Clear PTFE red rubber 5182 0717 Green polypropylene Clear PTFE red rubber 5182 0718 Red polypropylene Clear PTFE red rubber 5182 0719 Blue polypropylene Clear PTFE silicone 5182 0720 Green polypropylene Clear PTFE silicone 5182 0721 Red polypropylene Clear PTFE silicone 5182 0722 ...

Страница 38: ...rier ensure The transport assembly is parked see Park Arm Park Gripper on page 84 The vial tray is secured If the autosampler is to be shipped to another location the transport assembly must be moved to the park position to prevent mechanical damage should the shipping container be subjected to excessive shock Also ensure the vial tray is secured in place with suitable packaging otherwise the tray...

Страница 39: ...2 2 Optimizing Performance How to optimize the autosamplers to achieve best results ...

Страница 40: ...n the laboratories and the consistency and accuracy of analytical results The informations below will help you on how to optimize some parameters to achieve best results for lowest carry over for reliable quantitative data Fast injection cycles for high throughput Low delay volume for fast gradient Precise injection volume ...

Страница 41: ... of low concentration immediately after samples of high concentration carry over may become noticeable Using the automated needle wash enables the carry over to be minimized and prevents also contamination of the needle seat Using the Automated Needle Wash The automated needle wash can be programmed either as injection with needle wash or the needle wash can be included into the injector program W...

Страница 42: ...ain pass Using an Injector Program The process is based on a program that switches the bypass grove of the injection valve into the flow line for cleaning This switching event is performed at the end of the equilibration time to ensure that the bypass grove is filled with the start concentration of the mobile phase Otherwise the separation could be influenced especially if microbore columns are us...

Страница 43: ...d several wash vials can be used for cleaning In case the needle seat has got contaminated and carry over is significantly higher than expected the following procedure can be used to clean the needle seat Go to MORE INJECTOR and set needle to home position Pipette an appropriate solvent on to the needle seat The solvent should be able to dissolve the contamination If this is not known use 2 or 3 s...

Страница 44: ...duces the system delay volume the mobile phase is directed to the column without passing sample loop needle and needle seat capillary This can help to have faster cycle times especially if low flow rates have to be used like it is mandatory in narrow bore and micro bore HPLC NOTE Having the valve in bypass position can increase the carry over in the system The injection cycle times also depend on ...

Страница 45: ... to the sequence General Recommendations for Fast Injection Cycle Times As described in this section the first step to provide short cycle times are optimizing the chromatographic conditions If this is done the autosampler parameter should be set to Overlapped injection mode Increase of draw and eject speed for large injection volumes Add at last run a blank if overlapped injection is used To redu...

Страница 46: ...s sample out of the vial may have an influence on the injection volume precision when using viscous samples If the draw speed is too high air bubbles may form in the sample plug affecting precision The default draw speed is 200 µl min for the autosampler 4 µl min for the micro autosampler and 1000 µl min for the preparative autosampler This speed is suitable for the majority of applications howeve...

Страница 47: ...47 Optimizing Performance Precise Injection Volume the time required to run the injector program When using viscous samples a high eject speed should be avoided ...

Страница 48: ...the lifetime of the Vespel seal is drastically reduced a seal made of Tefzel is available see Injection Valve Assembly on page 168 Tefzel is more resistant than Vespel to extremes of pH however is a slightly softer material Under normal conditions the expected lifetime of the Tefzel seal is shorter than the Vespel seal however Tefzel may have the longer lifetime under more extreme mobile phase con...

Страница 49: ...3 3 Troubleshooting and Test Functions The modules built in troubleshooting and test functions ...

Страница 50: ...actions to fix the problem are provided see Error Messages on page 53 Maintenance Functions The maintenance functions position the needle arm gripper assembly and metering device for easy access when doing maintenance see Maintenance Functions on page 80 Tray Alignment Tray alignment is required after repair of internal components or after a firmware update The procedure aligns the gripper arm cor...

Страница 51: ...atus Indicators Two status indicators are located on the front of the autosampler The lower left indicates the power supply status the upper right indicates the autosampler status Figure 13 Location of Status Indicators Status indicator Power supply indicator ...

Страница 52: ... green status indicator indicates the instrument is performing an analysis run mode A yellow indicator indicates a not ready condition The instrument is in a not ready state when it is waiting for a specific condition to be reached or completed for example front cover not installed or while a self test procedure is running An error condition is indicated when the status indicator is red An error c...

Страница 53: ... before the analysis can be continued for example repair exchange of consumables is necessary In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the instrument log book This section describes the meaning of autosampler error messages and provides information on probable causes and suggested actions how to recover from erro...

Страница 54: ...eted successfully and the timeout function switched off the pump as requested A not ready condition was present during a sequence or multiple injection run for a period longer than the timeout threshold Suggested Actions Check the logbook for the occurrence and source of a not ready condition Restart the analysis where required ...

Страница 55: ... Probable Causes Leak detected in an external instrument with a remote connection to the system Shut down in an external instrument with a remote connection to the system The degasser failed to generate sufficient vacuum for solvent degassing Suggested Actions Fix the leak in the external instrument before restarting the autosampler Check external instruments for a shut down condition Check the de...

Страница 56: ...ot ready condition is still present on the remote line after one minute the error message is generated Probable Causes Not ready condition in one of the instruments connected to the remote line Defective remote cable Defective components in the instrument showing the not ready condition Suggested Actions Ensure the instrument showing the not ready condition is installed correctly and is set up cor...

Страница 57: ... configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable Causes CAN cable disconnected Defective CAN cable Defective main board in another module Suggested Actions Ensure all the CAN cables are connected correctly Switch off the system Restart the system and determine which module or modules are not recognized by ...

Страница 58: ...ther a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the ASM board Probable Causes Loose fittings Broken capillary Leaking rotor seal or needle seat Defective metering seal Suggested Actions Ensure all fittings are tight Exchange defective capillaries Exchange the rotor seal o...

Страница 59: ...t on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable Causes Leak sensor not connected to the ASM board Defective leak sensor Suggested Actions Ensure the leak sensor is connected correctly Exchange the leak sensor ...

Страница 60: ...e current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable Causes Defective leak sensor Suggested Actions Exchange the leak sensor ...

Страница 61: ...e resistance across the temperature compensation sensor NTC on the ASM board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable Causes Defective ASM board Suggested Actions Exchange the ASM board ...

Страница 62: ...t The resistance across the temperature compensation sensor NTC on the ASM board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable Causes Defective ASM board Suggested Actions Exchange the ASM board ...

Страница 63: ...fan shaft is used by the ASM board to monitor the fan speed If the fan speed falls below 2 revolutions second for longer than 5 seconds the error message is generated Probable Causes Fan cable disconnected Defective fan Defective ASM board Suggested Actions Ensure the fan is connected correctly Exchange fan Exchange the ASM board ...

Страница 64: ...n the ASM board detects when the top foam is in place If the foam is removed the fan is switched off and the error message is generated Probable Causes The top foam was removed during operation Foam not activating the sensor Sensor defective Suggested Actions Replace the top foam Exchange the ASM board ...

Страница 65: ... and foam open The sensor on the ASM board detects when the top foam is in place If the autosampler is restarted with the foam removed the autosampler switches off within 30 s and the error message is generated Probable Causes autosampler started with the top cover and foam removed Suggested Actions Replace the top cover and foam ...

Страница 66: ...rect position information from the encoders within the time window the error message is generated See figure Transport Assembly on page 210 for axes identification Arm Movement 0 Failed X axis Arm Movement 1 Failed Z axis Arm Movement 2 Failed Theta gripper rotation Arm Movement 3 Failed Gripper gripper fingers open close Probable Causes Mechanical obstruction High friction in transport assembly D...

Страница 67: ...valve is monitored by two microswitches on the valve assembly The switches detect the successful completion of the valve movement If the valve fails to reach the bypass position or if the microswitch does not close the error message is generated Probable Causes Defective injection valve Defective ASM board Suggested Actions Exchange the injection valve Exchange the ASM board ...

Страница 68: ...n valve is monitored by two microswitches on the valve assembly The switches detect the successful completion of the valve movement If the valve fails to reach the mainpass position or if the microswitch does not close the error message is generated Probable Causes Defective injection valve Defective ASM board Suggested Actions Exchange the injection valve Exchange the ASM board ...

Страница 69: ...etects the successful completion of the needle movement to the upper position If the needle fails to reach the end point or if the sensor fails to recognize the needle arm movement the error message is generated Probable Causes Defective or dirty position sensor Defective motor Sticking spindle assembly Defective ASM board Suggested Actions Exchange the sampling unit flex board Exchange the needle...

Страница 70: ...f the needle fails to reach the end point or if the sensor fails to recognize the needle arm movement the error message is generated Probable Causes Needle installed incorrectly or wrong needle type too long Defective or dirty position sensor Defective motor Sticking spindle assembly Defective ASM board Suggested Actions Ensure the correct needle type is used and installed correctly Exchange the s...

Страница 71: ...o vial is present the gripper fingers close too far This is sensed by the processor encoder position causing the error message to be generated Probable Causes No vial in the position defined in the method or sequence Incorrect gripper alignment Defective gripper assembly defective gripper fingers or belt Defective transport assembly flex board Suggested Actions Install the sample vial in the corre...

Страница 72: ...monitors the position sensors and motor encoders to check for correct movement If one or more of the movements is not successful or is not detected the error message is generated Probable Causes Mechanical obstruction Defective sampling unit flex board Defective transport assembly flex board Defective sampling unit motor Defective ASM board Suggested Actions Ensure unobstructed movement of the tra...

Страница 73: ... position of the plunger If the plunger fails to move to the home position or if the sensor fails to recognize the plunger position the error message is generated Probable Causes Dirty or defective sensor Broken plunger Defective metering drive motor Defective ASM board Suggested Actions Exchange the sampling unit flex board Exchange the metering plunger and seal Exchange the metering drive motor ...

Страница 74: ...r fingers The processor monitors the current drawn by each motor and the time the motor is drawing current The current drawn by the motors is dependent on the load on each motor friction mass of components etc If the current drawn is too high or the time the motor draws current is too long the error message is generated Probable Causes Mechanical obstruction High friction in the transport assembly...

Страница 75: ... correct operation of the gripper by closing and opening the gripper fingers while monitoring the motor encoder If a vial is still in the gripper when initialization is started the gripper fingers cannot close causing the error message to be generated Probable Causes Vial still in gripper Suggested Actions Remove the vial using the Release Vial function in the user interface Reinitialize the autos...

Страница 76: ...to inject sample the safety flap locks into position Next and the gripper checks the safety flap by trying to move the safety flap away from the needle If the gripper is able to move beyond the safety flap position safety flap not in position the error message is generated Probable Causes Safety flap missing or broken Suggested Actions Exchange the safety flap ...

Страница 77: ...uld be held by the gripper The autosampler checks if a sample vial is stuck in the gripper by closing and opening the gripper fingers while monitoring the motor encoder If the gripper fingers are unable to close the error message is generated Probable Causes Vial still in gripper Suggested Actions Remove the vial using the Release Vial function in the user interface Reinitialize the autosampler ...

Страница 78: ...ipper motor encoder If a vial is present the closing of the gripper fingers is limited by the vial However if no vial is present the gripper fingers close too far This is sensed by the processor encoder position causing the error message to be generated Probable Causes No wash vial in the position defined in the method Suggested Actions Install the wash vial in the correct position or edit the met...

Страница 79: ...sition does not exist in the current sample tray configuration the error message is generated Probable Causes Incorrect tray or trays installed Incorrect vial positions defined in the method or sequence Tray recognition defective dirty sample tray or defective transport assembly flex board Suggested Actions Install the correct trays or edit the method or sequence accordingly Ensure the coding surf...

Страница 80: ...he ChemStation and Control Module different names for functions in the Control Module are shown in brackets are Change Needle moves the safety flap away from the needle and positions the needle arm for easy access to the needle and needle seat Change Piston relieves the tension on the metering spring draws the piston to the outer position enabling easy disassembly of the metering head assembly Par...

Страница 81: ...e NOTE The autosampler front cover must be in place when Start and End are selected Start Change Moves the safety flap away from the needle and positions the needle approximately 15 mm above the needle seat Needle Up Up Arrow Press function key couple of times to move the needle arm up in 2 mm steps Needle Down Down Arrow Press function key couple of times to move the needle arm down in 2 mm steps...

Страница 82: ...over until the needle arm is in its maintenance position Removing the cover while the needle arm is activated may lock up the system 4 Exchange the needle or needle seat see Needle Seat Assembly on page 111 and Needle Assembly on page 108 5 Replace the front cover 6 Select End Done to complete the procedure ...

Страница 83: ...e commands for the ChemStation and Control Module different names for commands in the Control Module are shown in brackets are Start Change Draws the piston away from the home position relieving the tension on the spring End Done Repositions the plunger at the home position Using the Change Seal Function 1 Select Start Change to move the piston to the maintenance position 2 Exchange the metering s...

Страница 84: ...e parking the gripper arm ensure there is no vial in the gripper Use the Release Gripper function to remove the vial User Interface In the ChemStation the Park Arm command is part of the ALS maintenance positions that can be selected from the Maintenance menu in the Diagnosis display In the Control Module the Park Gripper command is located in the Control display of the autosampler The commands fo...

Страница 85: ... different names for commands in the Control Module are shown in brackets are Start Change Moves the transport assembly and gripper arm to the position required to change the gripper arm End Done Repositions the transport assembly and gripper arm to the home position Using the Change Seal Function 1 Select Start Change to move the gripper arm to the maintenance position 2 Exchange the gripper arm ...

Страница 86: ...tions Change Gripper Change Arm NOTE When the autosampler is switched on again the arm is initialized and moves out of the park position to the home position automatically No other action is required to prepare the autosampler for use ...

Страница 87: ... with firmware revision B 02 02 or higher The screen with the alignment dialog box can be found under the menus Views System Tests Autosampler The alignment procedure must be done with the standard 100 position vial tray installed WARNING The alignment procedure has to be performed in the correct order and without skipping parts to ensure a proper working Autosampler User Interface In the Control ...

Страница 88: ...nter of the gripper fingers and correct X and Theta position accordingly 9 Press the Enter key to hit Next Vial enter vial 95 and press Goto Vial 10 Repeat steps 4 to 8 to align the gripper at position 95 11 Press button Average F8 to balance the alignment 12 Press button Done F6 to store the alignment permanently in non volatile memory and to reset the module 13 To check the result go back to the...

Страница 89: ... specific failure mode or verify successful completion of a repair Each injector step command actually consists of a series of individual commands which move the autosampler components to predefined positions enabling the specific step to be done In the ChemStation the step commands can be selected from the Test Selection Box in the Diagnosis display In the Control Module the step commands can be ...

Страница 90: ...l at the seat and lifts the needle to the upper position Draw Metering device draws the defined injection volume Command also positions the vial at the seat lifts the needle and lowers the needle into vial Command can be done more than once maximum draw volume of 100µl cannot be exceeded Use Plunger Home to reset the metering device Needle Up Lifts the needle out of the vial Command also switches ...

Страница 91: ... position Command available fromControl Module only Table 14 Injector Step Commands continued Step Action Comments Table 15 Step Failures Step Function Probable Failure Modes Bypass Valve already in bypass Valve not connected Defective injection valve Plunger Home Defective or dirty sensor on the sampling unit flex board Defective metering drive motor Needle Up Needle already in the upper position...

Страница 92: ... not aligned see page 87 Needle Down Needle already in the lower position Defective or dirty sensor on the sampling unit flex board Sticking needle arm assembly Defective needle drive motor Mainpass Valve already in mainpass Valve not connected Defective injection valve Needle Up Mainpass Blockage in the sample loop or needle no solvent flow Needle already in the upper position Defective or dirty ...

Страница 93: ...93 Troubleshooting and Test Functions Step Commands ...

Страница 94: ...r fingers with error messages motor overtemp 0 or 1 or 2 or 3 movement failed 0 or 1 or 2 or 3 missing vial Many times the sampler is being used very heavily 2 Jittery shaky movement in X and or theta axes and or when the needle goes through the gripper arm into the vial with error messages motor overtemp 0 or 2 movement failed 0 or 2 3 Poor alignment seen during vial pickup and vial replacement a...

Страница 95: ...n vials used with the 1100 Autosampler must not have tapered shoulders or caps that are wider than the body of the vial For more details see the service note G1313 017 Step 3 Very heavy usage use a macro A pre sequence macro QMBUVHW_PDF will automatically reset the sampler at the start of a sequence ChemStation Step 4 Check if the INJECT line is used in the Injector Program Remove this line from t...

Страница 96: ...rt number G1313 60010 Step 8 Adjust the belt tension If the measured torque value is too low the belt needs to be tightened If the measured torque value is too high the belt needs to be loosened For this slide the motor X or theta on the holder bracket in the appropriate direction and test the tension with the torque2 macro Repeat this steps until the values are in the appropriate torque range Ste...

Страница 97: ... 03 61 resident A03 60 most of following errors movement failed motor over temp and initialization failed X axis are solved Step 2 Check the cleanliness of the transport rods X axis and clean them If the rod is dirty or sticky clean it with Isopropanol and wipe it with a lint free cloth The rod can be lubricated with the following synthetic oil part number 6040 0854 NOTE DO NOT use other lubricant...

Страница 98: ...gh the belt needs to be loosened For this slide the motor X or theta on the holder bracket in the appropriate direction and test the tension with the Torque2 mac macro Repeat this steps until the values are in the appropriate torque range Step 6 Reset the sampler alignments to default value Reset tray alignment and transport alignment is possible with the Control Module and the Chemstation To rese...

Страница 99: ...1 resident A03 60 most of following movement failed motor over temp and initialization failed X axis errors are solved Step 2 Reset the sampler alignment to default value Reset tray alignment and transport alignment is possible with the Control Module and the Chemstation To reset the transport alignment with the Chemstation enter following command in the command line Print sendmodule lals tray ali...

Страница 100: ...m exchange procedure is explained in the reference manual G1313 90004 section Repairing the Autosampler Step 6 Adjust the belts tension If the measured torque value is too low the belt needs to be tightened If the measured torque value is too high the belt needs to be loosened For this slide the motor on the holder bracket in the appropriate direction and test the tension with the Torque2 mac macr...

Страница 101: ...4 4 Repairing the Autosampler Instructions on simple routine repair procedures as well as more extensive repairs requiring exchange of internal parts ...

Страница 102: ... be removed from the instrument before opening the autosampler cover Do not connect the power cable to the autosampler while the covers are removed Safety Flap Flex Board It is strongly recommended that the exchange of the safety flap and flex board is done by Agilent trained service personnel Transport Assembly Parts The adjustment of the motors and the tension on the drive belts are important fo...

Страница 103: ...irmware The Agilent 1100 Series LC modules are fitted with FLASH EPROMS These EPROMS enable you to update the instrument firmware from the ChemStation PCMCIA card or through the RS232 interface The firmware update procedure is described in the on line user information ...

Страница 104: ...kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and a mild detergent Do not use an excessively damp cloth that liquid can drip into the autosampler WARNING Do not let liquid drip into the autosampler It could cause a shock hazard or damage to the autosampler ...

Страница 105: ...ap supplied in the accessory kit when handling electronic boards and components Using the ESD Strap 1 Unwrap the first two folds of the band and wrap the exposed adhesive side firmly around your wrist 2 Unroll the rest of the band and peel the liner from the copper foil at the opposite end 3 Attach the copper foil to a convenient and exposed electrical ground Figure 14 Using the ESD Strap ...

Страница 106: ... of Main Repair Procedures Overview of Main Repair Procedures Figure 15 Main Assemblies Needle page 108 Rotor seal page 111 Metering seal see page 123 Needle seat page 111 Transport assembly page 132 ASM board page 148 Power supply page 154 ...

Страница 107: ...15 minutes See Needle Assembly on page 108 Exchanging the seat assembly When the seat shows indication of damage or blockage 10 minutes See Needle Seat Assembly on page 111 Exchanging the rotor seal After approximately 30000 to 40000 injections or when the valve performance shows indication of leakage or wear 30 minutes See Rotor Seal on page 117 Exchanging the metering seal When autosampler repro...

Страница 108: ...it 2 5 mm Hex key supplied in accessory kit Parts required Needle assembly G1313 87201 for G1313 87101 or G1313 87103 needle seat Needle assembly G1329 80001 for G1329 87101 or G1329 87103 needle seat Needle assembly 900 µl loop cap G1313 87202 for G1313 87101 needle seat Needle assembly 900 µl loop cap G2260 87101 for G2260 87101 needle seat Before beginning this procedure Select Start in the mai...

Страница 109: ...ple loop fitting from the needle fitting 3 Loosen the fixing screw and lift out the needle 4 Select Needle Down Repeat the selection until the needle arm reaches its lowest position 5 Insert the new needle Align the needle in the seat then tighten the screw firmly ...

Страница 110: ...t the needle to a position approximately 2mm above the seat 8 Ensure the needle is aligned with the seat If required bend the needle slightly until the needle is aligned correctly On completion of this procedure Install the front cover Select End in the maintenance function Change Needle see Change Needle on page 81 CellFrame ...

Страница 111: ... mm i d 1 2 µl G1313 87103 for G1313 29A Needle seat assy 0 10 mm i d 1 2 µl G1329 87101 for G1389A Needle seat assy 0 05 mm i d 0 3 µl G1329 87103 for G1389A Needle seat assy 0 50 mm i d 20 µl G2260 87101 for G2260A Before beginning this procedure Select Start in the maintenance function Change Needle see Change Needle on page 81 Remove the front cover Use the Needle Up command in the Change Need...

Страница 112: ...ver to ease out the needle seat 3 Insert the new needle seat assembly Press the seat firmly into position 4 Connect the seat capillary fitting to port 5 of the injection valve 5 Use the Down button to position the needle approximately 2 mm above the seat CellFrame CellFrame CellFrame ...

Страница 113: ...e the needle is aligned with the seat If required bend the needle slightly until the needle is aligned correctly On completion of this procedure Install the front cover Select End in the maintenance function Change Needle see Change Needle on page 81 CellFrame ...

Страница 114: ...he valve Frequency Poor injection volume reproducibility Leaking injection valve Tools required 1 4 inch wrench supplied in accessory kit Hex key 9 64 inch supplied in accessory kit Parts required Stator face 0100 1851 for G1313A and G1329A Stator face 0101 1268 for G2260A Before beginning this procedure Remove the front cover 1 Remove all capillary fittings from the injection valve ports ...

Страница 115: ...bolts from the head 3 Remove the stator head and stator face 4 Place the stator face in place on the stator head Ensure the pins on the stator engage in the holes in the stator head 5 Install stator head and stator face Tighten the bolts alternately two turns at a time until the stator head is secure ...

Страница 116: ...ampler Stator Face 6 Reconnect the pump capillaries to the valve ports see Hydraulic Connections on page 31 7 Slide the waste tube into the waste holder in the leak tray On completion of this procedure Install the front cover ...

Страница 117: ...ility Leaking injection valve Tools required 1 4 inch wrench supplied in accessory kit Hex key 9 64 inch supplied in accessory kit Parts required Rotor seal 0100 1853 Vespel for G1313A and G1329A Rotor seal 0100 1849 Tefzel for G1313A and G1329A Rotor seal 0100 2088 Vespel for G1389A Rotor seal 0101 1268 PEEK for G2260A Before beginning this procedure Remove the front cover 1 Remove all capillary ...

Страница 118: ...ts from the head 3 Remove the stator head stator face and stator ring 4 Remove the rotor seal and isolation seal if required 5 Install the new rotor seal and isolation seal if required Ensure the metal spring inside the isolation seal faces towards the valve body CellFrame CellFrame CellFrame CellFrame ...

Страница 119: ...ce on the stator head 8 Install stator head and stator face Tighten the bolts alternately two turns at a time until the stator head is secure 9 Reconnect the pump capillaries to the valve ports see Hydraulic Connections on page 31 10 Slide the waste tube into the waste holder in the leak tray On completion of this procedure Install the front cover ...

Страница 120: ...0 µl analytical head Metering seal 5022 2175 pack of 1 for 40 µl analytical head Metering seal 0905 1294 pack of 1 for 900 µl analytical head Metering plunger 5063 6586 for 100 µl analytical head Metering plunger 5064 8293 for 40 µl analytical head Metering plunger 5062 8587 for 900 µl analytical head only if scratched or contaminated Before beginning this procedure Select Start in the maintenance...

Страница 121: ...g bolts from the base of the metering head assembly 4 Disassemble the metering head assembly 5 Use a small screwdriver to carefully remove the seal Clean the chamber with lint free cloth Ensure all particular matter is removed 6 Install the new seal Press the seal firmly into position 7 Place the piston guide on top of the seal CellFrame CellFrame CellFrame ...

Страница 122: ...to the seal 10 Install the fixing bolts Tighten the bolts securely 11 Install the metering head assembly in the autosampler Ensure the large hole in the metering head is facing downwards 12 Reinstall the capillaries On completion of this procedure Install the front cover Select End in the maintenance function Change piston see Change Piston on page 83 CellFrame ...

Страница 123: ...m on page 85 Turn off the power to the autosampler Remove the front cover 1 Identify the slit below the gripper motor and the gripper arm release button 2 Rotate the arm approximately 2 5cm 1 inch to the left and insert the straightened paper clip into the slit 3 Rotate the gripper arm slowly from left to right and apply a gentle pressure to the paper clip The clip will engage on an internal catch...

Страница 124: ... right 5 The gripper arm will come off 6 Replace the gripper arm by holding the paper clip in place pushing the gripper arm into the holder and rotating the gripper arm to the left On completion of this procedure Install the front cover Turn the power to the autosampler on CellFrame CellFrame CellFrame ...

Страница 125: ...er switch 2 Disconnect cables from the interface board connectors 3 Loosen the screws Slide out the interface board from the autosampler 4 Install the interface board Secure the screws 5 Reconnect the cables to the board connectors Figure 16 Exchanging the Interface Board Frequency At installation or when defective Tools required Flat head screwdriver Parts required Interface board see Optional In...

Страница 126: ...e observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used NOTE The electronics of the autosampler will not allow operation when the top cover and the top foam are removed A safety light switch on the main board will ...

Страница 127: ...once assembled incorrectly 1 Insert the Agilent Technologies 1100 Series nameplate into the recess in the top cover 2 Place the top cover on the bench 3 Press the side panels into the slots in the top cover Figure 17 Assembling the Main Cover Tools required None Parts required G1313 68703 Cover kit for G1313A G1329 68703 Cover kit for G1329A G1389A G2260A G1329 68706 Cabinet upgrade kit for G1313A...

Страница 128: ...hen handling electronic boards Frequency When accessing internal parts Tools required If interface board installed Flat head screwdriver Parts required Foam kit G1313 68702 1 Switch off the autosampler at the main power switch Remove the power cable 2 If installed remove the ECB board 3 Move the safety lever to the left Lift the clips on the top cover 4 Lift the cover away CellFrame safety lever c...

Страница 129: ...129 Repairing the Autosampler Top Cover and Foam 5 Unscrew the three screws on the top plate Remove the top plate 6 Carefully remove the top foam CellFrame CellFrame ...

Страница 130: ...nternal parts Tools required If interface board installed Flat head screwdriver Parts required None 1 Install the top foam Press the foam firmly into place 2 Engage the slot on the top plate onto the side plate 3 Fix the top plate in place with the three screws 4 Engage the top cover into the bottom cover CellFrame CellFrame ...

Страница 131: ...1 Repairing the Autosampler Installing the Top Cover and Foam 5 Lower the cover into place Ensure the clips close firmly 6 Slide the power lock to the right and install the power cable CellFrame CellFrame ...

Страница 132: ...require a flat head screwdriver to separate the transport assembly from the sampling unit Figure 18 Removing the Transport Assembly Frequency Sticking or jammed transport assembly Defective flex board or sensors Tools required If interface board installed Flat head screwdriver Parts required Transport assembly G1313 60009 for G1313A Transport assembly G1329 60009 for G1329A 1389A G2260A ...

Страница 133: ...nto the autosampler 2 Ensure the slot on the top of the transport assembly engages with the stud on the sampling unit On completion of this procedure Ensure the transport assembly is seated firmly Install the top cover and foam page 128 Verify the transport assembly alignment page 99 Transport assembly Sampling unit ...

Страница 134: ...g unit G1329 60008 for G1329A Sampling unit G1329 60018 for G1389A Sampling unit G2260 60008 for G2260A The sampling units come without injection valve and analytical head assembly see Sampling Unit Assembly on page 162 Before beginning this procedure Remove the front cover Remove the vial tray Remove the top cover page 128 Remove the transport assembly page 132 1 Remove the pump and column thermo...

Страница 135: ...he holder in the leak tray 3 Slide the sampling unit and tray base back to disengage the sampling unit connector 4 Lift the sampling unit and tray base approximately 10 cm Slide the leak sensor out of the leak plane 5 Lift the sampling unit and tray base out of the autosampler ...

Страница 136: ...136 Repairing the Autosampler Sampling Unit 6 Turn the tray base locking screw turn anti clockwise 7 Slide the tray base back to disengage the tray base from the sampling unit ...

Страница 137: ...pling unit remove the injection valve and metering head from the defective sampling unit Install the valve and metering head in the new sampling unit See Injection Valve Assembly on page 140 and Gripper Arm on page 123 1 Mount the tray base onto the sampling unit Ensure the tray base is positioned flush against the sampling unit 2 Tighten the locking screw turn clockwise Correct positioning of the...

Страница 138: ...k sensor into the holder in the leak plane 4 Place the sampling unit and tray base into the autosampler 5 Slide the sampling unit and tray base forwards Ensure the sampling unit connector is seated correctly 6 Install the pump and column thermostat capillaries ports 1 and 6 in the injection valve Connector ...

Страница 139: ...ing Unit 7 Install the waste tube in the holder in the leak tray On completion of this procedure Install the transport assembly page 132 Install the top cover page 128 Install the vial tray Verify the transport assembly alignment page 99 ...

Страница 140: ...329A Injection valve 0101 1050 for G1389A Injection valve 0101 1267 for G2260A Before beginning this procedure Remove all capillaries from the injection valve page 31 Remove the top cover page 128 Remove the transport assembly page 132 Remove the sampling unit page 134 1 Disconnect the valve cable connector from the SUD board labelled Valve Assy 2 Remove the three fixing screws 3 Remove the inject...

Страница 141: ...le to the connector labelled Valve Assy 6 Fix the valve assembly in place securely with the three screws On completion of this procedure Install the sampling unit page 134 Install the transport assembly page 132 Install the top cover page 128 Replace the injection valve capillaries page 31 Verify the transport assembly alignment page 99 ...

Страница 142: ...Pozidrive No 1 screwdriver Parts required Metering drive motor 5062 8590 Belt 1500 0697 Before beginning this procedure Remove the top cover page 128 Remove the transport assembly page 132 Remove the sampling unit page 134 7 Disconnect the motor connector from the SUD board labelled Metering M 8 Remove the four fixing screws 9 Remove the motor ...

Страница 143: ... SUD board Connect the cable to labelled Metering M 13 Install the motor with the four fixing screws Ensure the belt is seated correctly over the gear and motor shaft On completion of this procedure Install the sampling unit page 134 Install the transport assembly page 132 Install the top cover page 128 Verify the transport assembly alignment page 99 ...

Страница 144: ...required Pozidrive No 1 screwdriver Parts required Needle drive motor 5062 8590 Belt 1500 0697 Before beginning this procedure Remove the top cover page 128 14 Lift away the drive belt 15Disconnect the motor connector from the SUD board labelled Needle M 16Remove the metering head assembly see Gripper Arm on page 123 ...

Страница 145: ...le through to the SUD board Connect the cable to the connector labelled Needle M 19 Secure the motor in place with the four fixing screws 20 Install the belt 21 Install the metering head assembly see Gripper Arm on page 123 On completion of this procedure Install the top cover page 128 Verify the transport assembly alignment page 99 ...

Страница 146: ...lectronic boards Frequency When defective Tools required None Parts required Fan 3160 1017 Before beginning this procedure Remove the top cover and foam page 128 Remove the transport assembly page 132 1Disconnect the fan connector from the ASM board 2 Lift the fan and carefully pull the fan out of the foam 3 Install the new fan Ensure the cable is installed as shown CellFrame ...

Страница 147: ...Autosampler Fan 4 Connect the fan connector to the ASM board On completion of this procedure Install the transport assembly page 132 Install the top cover and foam page 128 Verify the transport assembly alignment page 99 ...

Страница 148: ...of the instrument serial number and realignment of the gripper Frequency When defective Tools required 5 mm wrench for remote connector screws 7 mm wrench for GPIB connector screws Parts required ASM board G1313 69520 for G1313A ASM board G1329 69500 for G1329A G1389A G2260A Before beginning this procedure Remove the top cover and foam page 128 Remove the transport assembly page 132 1 Remove the c...

Страница 149: ...B and RS232 connector screws On completion of this procedure On the new board check the switch setting of address switch S1 see Table 53 on page 236 or Table 54 on page 237 Note An incorrect switch setting e g TEST BOOT may cause the autosampler to turn in a basic mode yellow or red flashing status light In such a case turn off the pump re set the address switches and turn on the pump again CellFr...

Страница 150: ...ntrol module press System F5 then Records F4 Using the up down arrows make sure that the autosampler is highlighted 3 Press FW Update F5 Now press the m key This will display a box which says Update Enter Serial 4 Press Enter This will display the box labeled Serial 5 Letters and numbers are created using the up and down arrows Into the box labeled Serial enter the 10 character serial number for t...

Страница 151: ... entered is different than the original module serial number you will be given the opportunity to edit the configure Agilent 1100 access screen during the re boot of the ChemStation 3 After boot up the serial number you have just entered can be seen under the Instrument menu of the main user interface screen The serial number of the autosampler can also be seen by typing the following command into...

Страница 152: ...1 screwdriver Parts required SUD board G1313 66503 Before beginning this procedure Remove the top cover page 128 Remove the transport assembly page 132 Remove the sampling unit page 134 1 Disconnect all connectors from the SUD board 2 Remove the two fixing screws and lift out the board 3 Install the new board Ensure the board is positioned between the board guide Fixing screws ...

Страница 153: ...all the connectors to the new board On completion of this procedure Install the sampling unit page 134 Install the transport assembly page 132 Install the top cover page 128 Verify the transport assembly alignment page 99 Fixing screws Leak sensor Metering drive Injection Valve Needle drive Flex board ...

Страница 154: ...Tools required Pozidrive No 1 screwdriver Parts required Power supply 0950 2528 Before beginning this procedure Remove the top cover page 128 Remove the transport assembly page 132 Remove the sampling unit page 134 Remove the ASM board page 148 Remove the fan page 146 1 Remove the bottom foam 2Remove the power switch light pipe from the coupler 3Remove the two screws from the rear panel CellFrame ...

Страница 155: ...e coupler from the of the power supply 6 Insert the coupler onto the switch of the new power supply 7 Install the power supply in the module 8 Connect the light pipe to the coupler 9 Install the bottom foam Ensure the cables are positioned as shown CellFrame CellFrame CellFrame CellFrame CellFrame ...

Страница 156: ...y On completion of this procedure Install the ASM board page 148 Install the fan page 146 Install the sampling unit page 134 Install the transport assembly page 132 Install the top cover page 128 Verify the transport assembly alignment page 99 ...

Страница 157: ...his procedure Remove the top cover page 128 Remove the transport assembly page 132 Remove the sampling unit page 134 1 Disconnect the leak sensor from the SUD board labelled leak sensor Remove the sensor 2 Feed the cable of the new sensor through the base of the sampling unit to the SUD board 3 Connect the connector to the connector labelled leak sensor ...

Страница 158: ... the Autosampler Leak Sensor On completion of this procedure Install the sampling unit page 134 Install the transport assembly page 132 Install the top cover page 128 Verify the transport assembly alignment page 99 ...

Страница 159: ...5 5 Parts and Materials Detailed illustrations and lists for identification of parts and materials ...

Страница 160: ...160 Parts and Materials Main Assemblies Main Assemblies Figure 19 Autosampler Main Assemblies 5 3 4 2 1 9 8 7 6 ...

Страница 161: ... 29A Injection valve assembly for G1389A Injection valve assembly for G2260A 0101 0921 0101 1050 0101 1267 5 Vial tray thermostatted see page 175 G1329 60001 6 Gripper assembly G1313 60010 7 Autosampler Main Board ASM for G1313 Autosampler Main Board ASM for G1329A 1389A 2260A G1313 69520 G1329 69500 Standoff GPIB connector part not shown 0380 0643 Standoff remote connector part not shown 1251 778...

Страница 162: ...162 Parts and Materials Sampling Unit Assembly Sampling Unit Assembly Figure 20 Autosampler Sampling Unit Assembly 1 2 15 14 13 12 11 8 7 10 6 4 3 9 5 ...

Страница 163: ...ry 01078 87302 G1329 87302 G1375 87303 G1313 87303 5022 2133 5 Analytical head assembly 100 µl for G1313 29A Analytical head assembly 40 µl for G1389A Preparative head assembly 900 µl for G1313 29A P 200Bar Preparative head assembly 900 µl for G2260A P 400Bar 01078 60003 G1377 60013 G1313 60007 G2260 60007 6 Inj valve Anal head cap 160 mm 0 25 mm for G1313 29A Inj valve Anal head cap 200 mm 0 05 m...

Страница 164: ...01 G1329 87103 G2260 87101 12 Seat adapter G1313 43204 13 Safety flap G1313 44106 14 Flex board G1313 68715 15 Needle assembly for G1313 87101 or G1313 87103 needle seat Needle assembly for G1329 87101 or G1329 87103 needle seat Needle assembly 900 µl loop capillary for G1313 87101 needle seat Needle assembly 900 µl loop capillary for G2260 87101 needle seat G1313 87201 G1329 80001 G1313 87202 G22...

Страница 165: ...embly 5001 3739 5 Metering seal pack of 2 5063 6589 6 Head body 01078 27710 Screw M5 60 mm lg for mounting of assembly 0515 2118 Table 20 Analytical Head Assembly 40 µl for G1389A WHP HVFULSWLRQ 3DUW 1XPEHU Micro Analytical head assembly 40 µl includes items 1 6 G1377 60013 1 Micro Plunger assembly 5064 8293 2 Screws 0515 0850 3 Adapter 01078 23202 4 Micro seal support assembly G1377 60002 5 Meter...

Страница 166: ...166 Parts and Materials Analytical Head Assembly Figure 21 Analytical Head Assembly 2 3 4 5 6 1 ...

Страница 167: ...y 900 µl 5001 3764 5 Metering seal 900 µl 0905 1294 6 Head body 900 µl G1313 27700 Screw M5 60 mm lg for mounting of assembly 0515 2118 WHP HVFULSWLRQ 3DUW 1XPEHU Analytical head assembly 900 µl includes items 1 6 This head is limited to 400 Bars It can only be assembled on a sampling unit with the description supports 900 µl at 400 Bar G2260 60007 1 Plunger assembly 900 µl 5062 8587 2 Screws 0515...

Страница 168: ...or seal Vespel 0100 1853 3 Rotor seal Tefzel 0100 1849 4 Stator face 0100 1851 5 Stator head 0100 1850 6 Stator screws 1535 4857 Table 24 Micro Injection Valve Assembly for G1389A The micro injection valve assembly has no ceramic stator face WHP HVFULSWLRQ 3DUW 1XPEHU 1 Micro Inj valve assy incl items 1 2 3 5 6 0101 1050 2 Isolation seal 0100 1852 3 Micro rotor seal Vespel 0100 2088 5 Micro Stator...

Страница 169: ...igure 22 Injection Valve Assembly WHP HVFULSWLRQ 3DUW 1XPEHU 1 MBB Injection valve assembly includes items1 6 MBB Make Before Brake is a trademark by Rheodyne 0101 1267 2 Isolation seal 0100 1852 3 4 Rotor seal PEEK Stator face PEEK kit 0101 1268 5 Stator head 0100 2195 6 Stator screws 1535 4857 1 2 6 3 5 4 ...

Страница 170: ...al Kit Figure 23 Sheet Metal Kit Table 26 Sheet Metal WHP HVFULSWLRQ 3DUW 1XPEHU 1 Slot cover 5001 3772 2 Screw cover 5022 2112 3 Autosampler Sheet metal kit for G1313A Autosampler Sheet metal kit for G1329A G1389A G2260A G1313 68701 G1329 68701 2 3 1 ...

Страница 171: ...G1313 68703 G1329 68703 2 Name plate for Agilent 1100 Series 5042 1381 3 Transparent front cover G1313 68704 4 Door repair kit includes transparent side and front door G1329 68707 5 Light protection kit includes opaque side and front door opaque front cover G1329 68708 Cabinet upgrade kit includes side panels top cover transparent side and front door front cover and side insulation cover for coole...

Страница 172: ...ls Foam Parts Foam Parts Figure 25 Foam Parts Table 28 Foam Parts WHP HVFULSWLRQ 3DUW 1XPEHU Foam kit includes items 2 and 3 G1313 68702 1 Board guides 5041 8395 2 Top foam Order foam kit 3 Bottom foam Order foam kit 2 3 1 ...

Страница 173: ...tatus Light Pipes Figure 26 Power and Status Light Pipes Table 29 Power and Status Light Pipes WHP HVFULSWLRQ 3DUW 1XPEHU 1 Power switch button 5041 8381 2 Light pipe power switch 5041 8382 3 Power switch coupler 5041 8383 4 Light pipe status lamp 5041 8384 1 2 3 4 ...

Страница 174: ...em Parts Figure 27 Leak System Parts Table 30 Leak System Parts WHP HVFULSWLRQ 3DUW 1XPEHU 1 Leak funnel holder 5041 8389 2 Leak sensor 5061 3356 3 Leak plane G1313 44501 4 Leak tubing 120 mm reorder gives 5 m 5062 2463 5 Leak funnel 5041 8388 1 5 4 3 2 ...

Страница 175: ...utosampler Vial Trays and Tray Base WHP HVFULSWLRQ 3DUW 1XPEHU 1 Tray for 100 2 ml vials G1313 44500 2 Halftray for 15 6 ml vials G1313 44503 3 Halftray for 40 2 ml vials G1313 44502 4 Spring G1313 09101 5 Spring stud 0570 1574 6 Tray base for G1313A includes items 4 5 G1313 60002 3 1 2 6 4 5 ...

Страница 176: ...Table 32 Thermostatted Autosampler Vial Trays and Tray Base WHP HVFULSWLRQ 3DUW 1XPEHU 1 Adapter air channel G1329 43200 2 Tray for 100 2 ml vials thermostattable G1329 60001 3 Spring G1313 09101 4 Tray base for G1329A G1389A G2260A includes items 4 5 G1329 60000 5 Spring stud 0570 1574 4 2 1 3 5 ...

Страница 177: ...0714 Blue screw caps 100 pk 5182 0717 Label halftray no PN Vial instruction sheet no PN Wrenches 1 4 5 16 inch 8710 0510 Rheotool socket wrench 1 4 inch 8710 2391 Hex key 4 mm 15 cm long T handle 8710 2392 Hex key 9 64 mm 15 cm long T handle 8710 2394 Hex key 2 5 mm 15 cm long straight handle 8710 2412 ESD wrist strap no PN Finger caps x3 Reorder gives pack of 15 5063 6506 Tray for 40 x 2 ml vials...

Страница 178: ... 5182 0717 Label halftray no PN Vial instruction sheet no PN Wrenches 1 4 5 16 inch 8710 0510 Rheotool socket wrench 1 4 inch 8710 2391 Hex key 4 mm 15 cm long T handle 8710 2392 Hex key 9 64 mm 15 cm long T handle 8710 2394 Hex key 2 5 mm 15 cm long straight handle 8710 2412 ESD wrist strap no PN Finger caps x3 reorder gives pack of 15 5063 6506 Front door cooled autosampler no PN Air channel ada...

Страница 179: ...abel halftray no PN Fitting 5061 3303 Hex Key 8710 0060 Wrench 4 mm both ends 8710 1534 Wrenches 1 4 5 16 inch 8710 0510 Rheotool socket wrench 1 4 inch 8710 2391 Hex key 4 mm 15 cm long T handle 8710 2392 Hex key 9 64 mm 15 cm long T handle 8710 2394 Hex key 2 5 mm 15 cm long straight handle 8710 2412 ESD wrist strap no PN Finger caps x3 reorder gives pack of 15 5063 6506 Torque adapter G1315 450...

Страница 180: ...es 1 4 5 16 inch 8710 0510 Rheotool socket wrench 1 4 inch 8710 2391 Hex key 4 mm 15 cm long T handle 8710 2392 Hex key 9 64 mm 15 cm long T handle 8710 2394 Hex key 2 5 mm 15 cm long straight handle 8710 2412 ESD wrist strap no PN Finger caps x3 reorder gives pack of 15 5063 6506 Front door cooled autosampler no PN Air channel adapter G1329 43200 Tray for 15 x 6 ml vials x2 G1313 44503 Union loop...

Страница 181: ...709 Maintenance Kit G1313 68709 Table 37 Maintenance Kit WHP HVFULSWLRQ 3DUW 1XPEHU 1 Rotor seal Vespel 0100 1853 2 Needle assembly G1313 87201 3 Needle seat assembly G1313 87101 4 Metering seal pack of 2 5063 6589 5 Finger caps pack of 15 5063 6506 ...

Страница 182: ...ials Multi Draw Kit G1313 68711 Multi Draw Kit G1313 68711 Table 38 Multi Draw Kit WHP HVFULSWLRQ 3DUW 1XPEHU 1 Seat capillary 500 µl 0 5 mm id G1313 87307 2 Seat capillary 1500 µl 0 9 mm id G1313 87308 3 Union 0100 0900 ...

Страница 183: ...or G1313A 29A 900 µl Injection Upgrade Kit G1363A for G1313A 29A Table 39 900 µl Injection Upgrade Kit WHP HVFULSWLRQ 3DUW 1XPEHU 1 Analytical Head 900 µl G1313 60007 2 Loop Extension 900 µl G1313 87303 3 Union loop extension 5022 2133 4 Needle 900 µl G1313 87202 ...

Страница 184: ...184 Parts and Materials External Tray G1313 60004 External Tray G1313 60004 Table 40 External Tray WHP HVFULSWLRQ 3DUW 1XPEHU 1 External tray G1313 60004 2 Disposal tube G1313 27302 ...

Страница 185: ...s 3390 2 3 integrators 01040 60101 3394 6 integrators 35900 60750 Agilent 35900A A D converter 35900 60750 General purpose spade lugs 01046 60105 Remote cables 3390 integrator 01046 60203 3392 3 integrators 01046 60206 3394 integrator 01046 60210 3396A Series I integrator 03394 60600 3396 Series II 3395A integrator see page 192 3396 Series III 3395B integrator 03396 61010 HP 1050 modules HP 1046A ...

Страница 186: ... 1516 5181 1519 Agilent 1100 module to control module G1323 81600 External contacts Agilent 1100 Series interface board to general purpose G1103 61611 GPIB cable Agilent 1100 module to ChemStation 1 m 10833A Agilent 1100 module to ChemStation 2 m 10833B RS 232 cable Agilent 1100 module to a computer This kit contains a 9 pin female to 9 pin female Null Modem printer cable and one adapter 34398A LA...

Страница 187: ...connection is being made Agilent 1100 to 3390 2 3 Integrators Agilent 1100 to 3394 6 Integrators Connector 01040 60101 Pin 3390 2 3 Pin Agilent 1100 Signal Name 1 Shield Ground 2 Not connected 3 Center Signal 4 Connected to pin 6 5 Shield Analog 6 Connected to pin 4 7 Key 8 Not connected Connector 35900 60750 Pin 3394 6 Pin Agilent 1100 Signal Name 1 Not connected 2 Shield Analog 3 Center Analog ...

Страница 188: ...to BNC Connector Agilent 1100 to General Purpose Connector 8120 1840 Pin BNC Pin Agilent 1100 Signal Name Shield Shield Analog Center Center Analog Connector 01046 60105 Pin 3394 6 Pin Agilent 1100 Signal Name 1 Not connected 2 Black Analog 3 Red Analog ...

Страница 189: ...eries modules The other end depends on the instrument to be connected to Agilent 1100 to 3390 Integrators Connector 01046 60203 Pin 3390 Pin Agilent 1100 Signal Name Active TTL 2 1 White Digital ground NC 2 Brown Prepare run Low 7 3 Gray Start Low NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6 Yellow Power on High NC 7 Red Ready High NC 8 Green Stop Low NC 9 Black Start request Low ...

Страница 190: ... NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6 Yellow Power on High 9 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 4 Key Connector 01046 60210 Pin 3394 Pin Agilent 1100 Signal Name Active TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 3 3 Gray Start Low NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6 Yellow Power on High 5 14 7 Red Ready High 6 8 Green Stop...

Страница 191: ...191 Parts and Materials Remote Cables NOTE START and STOP are connected via diodes to pin 3 of the 3394 connector ...

Страница 192: ...integrator side Otherwise the integrator prints START not ready Connector 03394 60600 Pin 3394 Pin Agilent 1100 Signal Name Active TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 3 3 Gray Start Low NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6 Yellow Power on High 5 14 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected ...

Страница 193: ...ow NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6 Yellow Power on High 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Connector 5061 3378 Pin HP 1050 Pin Agilent 1100 Signal Name Active TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Po...

Страница 194: ...n Low 4 3 Gray Start Low 7 4 Blue Shut down Low 8 5 Pink Not connected NC 6 Yellow Power on High 3 7 Red Ready High 6 8 Green Stop Low NC 9 Black Start request Low 5 Key Connector 01046 60201 Pin Universal Pin Agilent 1100 Signal Name Active TTL 1 White Digital ground 2 Brown Prepare run Low 3 Gray Start Low 4 Blue Shut down Low 5 Pink Not connected 6 Yellow Power on High 7 Red Ready High 8 Green ...

Страница 195: ...ent 1100 Series modules The other end depends on the instrument to be connected to Agilent 1100 to 3392 3 Integrators Connector 18584 60510 Pin 3392 3 Pin Agilent 1100 Signal Name BCD Digit 10 1 BCD 5 20 11 2 BCD 7 80 3 3 BCD 6 40 9 4 BCD 4 10 7 5 BCD 0 1 5 6 BCD 3 8 12 7 BCD 2 4 4 8 BCD 1 2 1 9 Digital ground 2 15 5 V Low 6 Key ...

Страница 196: ... 1 BCD 5 20 2 2 BCD 7 80 3 3 BCD 6 40 4 4 BCD 4 10 5 5 BCD 0 1 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5 V Low Connector 18594 60520 Wire Color Pin Agilent 1100 Signal Name BCD Digit Green 1 BCD 5 20 Violet 2 BCD 7 80 Blue 3 BCD 6 40 Yellow 4 BCD 4 10 Black 5 BCD 0 1 Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground White 15 5 Vt Low ...

Страница 197: ...to general purposes CAN Cable Both ends of this cable provide a modular plug to be connected to Agilent 1100 Series module s CAN bus connectors Connector G1322 81600 Color Pin Agilent 1100 Signal Name White 1 Ground Brown 2 Pressure signal Green 3 Yellow 4 Grey 5 DC 5 V IN Pink 6 Vent Agilent 1100 module to module 0 5m lg 5181 1516 Agilent 1100 module to module 1m lg 5181 1519 Agilent 1100 module ...

Страница 198: ...urpose Agilent 1100 Series Interface Board to general purposes 5 10 15 1 11 6 RQQHFWRU RORU 3LQ JLOHQW 6LJQDO 1DPH White 1 EXT 1 Brown 2 EXT 1 Green 3 EXT 2 Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT 3 Blue 7 EXT 4 Red 8 EXT 4 Black 9 Not connected Violet 10 Not connected Grey pink 11 Not connected Red blue 12 Not connected White green 13 Not connected Brown green 14 Not connected White yellow 156 Not...

Страница 199: ...adapter Use the cable and adapter to connect Agilent Technologies instruments with 9 pin male RS 232 connectors to most PCs or printers Agilent 1100 module to PC RS 232 Cable Kit 34398A Instrument DCD RX TX DTR GND DSR RTS CTS RI DCD RX TX DTR GND DSR RTS CTS RI PC 1 2 3 4 5 6 7 8 9 1 2 3 4 5 6 7 8 9 DB9 Male DB9 Female DB9 Female DB9 Male ...

Страница 200: ...ables Recommended Cables For point to point connection not using a network hub use a twisted pair cross over LAN cable P N 5183 4649 10 feet long For standard network connections using a hub use category 5 UTP cables P N G1530 61480 8 m long ...

Страница 201: ...6 6 Introduction to the Autosampler An introduction to the standard micro and preparative autosamplers and thermostatted autosamplers ...

Страница 202: ...use with the thermostatted autosamplers The half size racks trays are not designed for an optimal heat transfer when they are used with a thermostatted autosampler The autosamplers transport mechanism uses an X Z Theta movement to optimize vial pick up and return Vials are picked up by the gripper arm and positioned below the sampling unit The gripper transport mechanism and sampling unit are driv...

Страница 203: ... available Control of the vial temperature in the thermostatted autosampler is achieved using an additional Agilent 1100 Series module the ALS thermostat Details of this module are given in the Agilent 1100 Series thermostatted autosampler Supplemental Manual Figure 30 Overview of the Autosampler Switching Valve Needle Transport assembly ASM board Power supply Sampling Unit Vial Tray Gripper Arm A...

Страница 204: ... the mainpass position at the end of the sampling sequence The sampling sequence occurs in the following order 1 The injection valve switches to the bypass position 2 The plunger of the metering device moves to the initialization position 3 The gripper arm moves from the home position and selects the vial At the same time the needle lifts out of the seat 4 The gripper arm places the vial below the...

Страница 205: ...bile phase flows through the autosamplers metering device sample loop and needle ensuring all parts in contact with sample are flushed during the run thus minimizing carry over Figure 31 Mainpass Position When the sample sequence begins the valve unit switches to the bypass position Figure 32 Solvent from the pump enters the valve unit at port 1 and flows directly to the column through port 6 Figu...

Страница 206: ...to the sample loop Figure 33 Figure 33 Drawing the Sample When the metering unit has drawn the required volume of sample into the sample loop the needle is raised and the vial is replaced in the sample tray The needle is lowered into the needle seat and the injection valve switches back to the mainpass position flushing the sample onto the column Figure 34 Figure 34 Mainpass Position Sample Inject...

Страница 207: ...ive metering device and injection valve NOTE The replacement sampling unit excludes the injection valve and metering head assemblies The sampling units for the standard the thermostatted the micro and the preparative autosamplers are different Figure 35 Autosampler Sampling Unit Metering device Injection valve Needle drive ...

Страница 208: ... pushes the sapphire plunger against the tension of the spring into the analytical head The base of the plunger sits on the large bearing of the drive nut which ensures the plunger is always centered A ceramic ring guides the movement of the plunger in the analytical head The home position of the plunger is sensed by an infra red sensor on the sampling unit flex board while the sample volume is de...

Страница 209: ...ection valve Two microswitches monitor switching of the valve bypass and mainpass end positions No valve adjustments are required after replacing internal components Table 43 Injection Valve Technical Data Standard Micro Preparative MBB Motor type 4V 1 2A stepper motor 4V 1 2A stepper motor 4V 1 2A stepper motor Seal material Vespel Tefzel available Vespel PEEK Stator material Ceramic PEEK Head co...

Страница 210: ...or the gripper assembly for sample vial transport The rotational movement of the motors is converted to linear motion X and Z axes by toothed belts connected to the drive spindles The rotation theta axes of the gripper assembly is transferred from the motor by a toothed belt and series of gears The opening and closing of the gripper fingers are driven by a stepper motor linked by a toothed belt to...

Страница 211: ...r position errors automatically e g if the gripper is accidentally moved out of position when loading vials into the vial tray The initialization positions of the moving components are sensed by reflection sensors mounted on the flex board These positions are used by the processor to calculate the actual motor position An additional six reflection sensors for tray recognition are mounted on the fl...

Страница 212: ...F counters Each counter increments with autosamplers use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Each counter can be reset to zero after maintenance has been done The autosamplers provides the following EMF counters Injection valve counter This counter display the total number of switches of the injection valve since the l...

Страница 213: ... instrument performance indicates maintenance is necessary make note of the values displayed by the injection valve and needle movements counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs...

Страница 214: ...lers The switches are preset to a default address see Table 50 on page 232 which is recognized once after power on The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal Agilent 1100 Series module data transfer and synchronization The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies i...

Страница 215: ...onnect or reconnect the autosampler to ALS thermostat cable when the power cords are connected to either of the two modules This will damage the electronics of the modules The power input socket accepts a line voltage of 100 120 or 220 240 volts AC 10 with a line frequency of 50 or 60 Hz There is no voltage selector on your autosamplers because the power supply has wide ranging capability There ar...

Страница 216: ...216 Introduction to the Autosampler Electrical Connections ...

Страница 217: ...7 7 Theory of Operation Theory of operation of mechanical hardware electronics and instrument interfaces ...

Страница 218: ...sport mechanism sampling needle metering unit and high speed injection valve These devices are controlled by a versatile electronics design based upon a 68000 family processor which also contains battery backup RAM flash ROM a real time clock and several communications options ...

Страница 219: ...ort Flex Board 6HQVRU 7 SH 1XPEHU RI 6HQVRUV 3RVLWLRQ 0RYHPHQW 6HQVHG Reflection Sensor 6 Vial tray identification Reflection Sensor 1 Gripper initialization Reflection Sensor 3 Transport assembly Initialization Table 45 Sampling Unit Flex Board 6HQVRU 7 SH 1XPEHU RI 6HQVRUV 3RVLWLRQ 0RYHPHQW 6HQVHG IR light sensor 1 Metering device home reference position Reflection sensor 2 Needle end positions ...

Страница 220: ...ctors on this board and the 8 bit configuration switch which is used to configure the address for the GPIB communication baud rate for RS 232 transfer and so on Also the ASIC controls and drives module specific functions and reads static status signals Leak Converter Solvent leaking from the autosampler cools down the PTC This changes the resistance of the PTC causing the leak converter to generat...

Страница 221: ...thium Batteries Information on page 282 Autosampler Specific Electronics The autosampler specific functions provided by the electronics are closed loop control of four axis vial handling servos electric valve control Needle unit control metering device control Transport Unit Control The transport drive electronics use current controlled pulse width modulation PWM to drive the X Z θ and gripper mot...

Страница 222: ... of Operation Autosampler Main Board ASM reflection light sensors are located Six light sensors are used for vial tray identification one for gripper decoding and three for decoding of the initialization position ...

Страница 223: ...sing of the valve motor is done by two microswitches Two reflection light sensors are used to detect the end positions of the needle arm One photo sensor is required to detect the home position of the metering device Two hall sensors detect correct closure of the door needle arm movement is interrupted if the door is open All the sensors are mounted on one flex board The flex board and motors are ...

Страница 224: ...r Circuits Needle Drive 2A Phase Valve Drive 4 5A Phase Mixing Drive 0 6A Phase Metering Drive 2A Phase Needle End Position Sensors 2 Metering Home Position Sensor Safety Hall Sensors 2 Sampling Unit Distribution Board Valve End Position Sensors 2 Sampler Main Board Via sampling unit flex board ...

Страница 225: ...theta 1 Z Axis X Axis Sampling Unit Metering Device Injection Valve Core common Resources DSP theta 2 Limit switch signals 8 bit data 5 bit address Encoder feedback data Quadrature decoder Serial data Encoder clocks and direction FPGAchip select 5 bit address 8 bit data TPU signals I O signals ...

Страница 226: ...communication capabilities GPIB CAN LAN and RS 232C memory management ability to update the firmware of the main system Main System Its properties are the complete communication capabilities GPIB CAN LAN and RS 232C memory management ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common functions like run s...

Страница 227: ...onventions are xxxx vvv DLB where xxxx is the product number e g 1313 for the G1313A Autosampler and vvv is the revision number for example 380 is revision 3 80 For instructions refer to your user interface NOTE Update of main system can be done in the resident system only Update of the resident system can be done in the main system only Figure 41 Firmware Update Mechanism Resident System Main Sys...

Страница 228: ...ies autosampler and four external contacts The external contact closure contacts are relay contacts The maximum settings are 30 V AC DC 250 mA fused There are general purpose cables available to connect the BCD output see BCD Cables on page 195 and the external outputs see External Contact Cable on page 198 to external devices Table 47 Optional Interface Boards HVFULSWLRQ 3DUW 1XPEHU BCD Board G13...

Страница 229: ...commended to add the LAN board to the detector with highest data rate NOTE The LAN board can only be used together with a main board version G13XX 66520 DAD MWD VWD Pump ALS or G13XX 66500 FLD RID and above a Agilent ChemStation software revision A 06 01 or above Processor interface Board identification BCD register External contacts Line driver 12 4 x 250 mA BCD connector External contact connect...

Страница 230: ...t connection not using a network hub use a twisted pair cross over LAN cable P N 5183 4649 10 feet long For standard network connections using a hub use category 5 UTP cables P N G1530 61480 8 m long Table 48 LAN Boards JLOHQW 2UGHU 1XPEHU 6XSSRUWHG QHWZRUNV J4106A Ethernet 802 3 RJ 45 10Base T J4105A Token Ring 802 5 DB9 RJ 45 10Base T J4100A Fast Ethernet Ethernet 802 3 RJ 45 10 100Base TX BNC 1...

Страница 231: ...tors Figure 6 on page 27 WARNING Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations see External Tray G1313 60004 on page 184 Table 49 Agilent 1100 Series Interfaces Interface Type Pumps Autosampler DA Detector MW Detector FL Detector VW Detector RI Detector Thermostatted Column Compartment Vacuum Degas...

Страница 232: ...wire serial bus system supporting high speed data communication and real time requirement Remote Interface The APG remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as common shut down prepare and so on Remote control allows easy connection between single instruments or systems to ensure coordinated analysis wit...

Страница 233: ...e line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal level is defined as standard TTL levels 0 V is logic true 5 V is false fan out is 10 input load is 2...

Страница 234: ... problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish an...

Страница 235: ...GPIB connector The RS 232C is designed as DCE Data Communication Equipment with a 9 pin male SUB D type connector The pins are defined as follows Table 52 RS 232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 9 In RI RS 232 Cable Instrument DCD RX TX DTR GND DSR RTS CTS RI DCD RX TX DTR GND DSR RTS CTS RI PC 1 2 3 4 5 6 7 8 9 1 2...

Страница 236: ...h set of parameters for example for GPIB RS 232C and so on will be changed Once the change has been completed the instrument must be powered up again in order to store the values in the non volatile memory In the non volatile memory the parameters are kept regardless of whether you turn the instrument off and on again They will be kept until the same Table 53 8 bit Configuration Switch Mode Select...

Страница 237: ...dresses If you just want to change the GPIB address and need a detailed procedure refer to the Installing Your Agilent ChemStation System handbook Default GPIB address is set to the following addresses where 0 means that the switch is down and 1 means that the switch is up Table 54 Default Addresses for Agilent Series 1100 Modules 0RGXOH GGUHVV LQDU GGUHVV Pump 22 0 0 0 1 0 1 1 0 FLD 23 0 0 0 1 0 ...

Страница 238: ... Use the following tables for selecting the setting which you want to use for RS 232C communication The number 0 means that the switch is down and 1 means that the switch is up One start bit and one stop bit are always used not selectable Table 55 Communication Settings for RS 232C Communication Mode Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baud rate Data Bits Parity Table 56 Baud Rate Settings Switches...

Страница 239: ... data stored in the non volatile memory Exceptions are diagnose and repair log books which will not be erased If you use the following switch settings and power the instrument up again a forced cold start has been completed To return to normal operation set switches back to your GPIB or RS 232 configuration settings Stay Resident Settings Firmware update procedures may require this mode in case of...

Страница 240: ...l operation If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident part that is it is not operable as a detector It only uses basic functions of the operating system for example for communication To return to normal operation set switches back to your GPIB or RS 232C configuration settings Table 60 Stay Resident Settings Mode Select...

Страница 241: ...a closed assembly no onsite repair possibility The power supply provides all DC voltages used in the module except for the voltages supplied by the lamp power supply to the deuterium and tungsten lamps in the detectors The line voltage can vary in a range from 100 120 or 220 240 volts AC 10 and needs no manual setting ...

Страница 242: ...r Supply MPS Blockdiagram 3 6 V s u p p l y 2 4 V s u p p l y 5 V s w i t c h i n g r e g u l a t o r l i n e a r v o l t a g e r e g u l a t o r s 3 6 V n o t u s e d 2 4 V 4 5 A 5 V 3 A 1 5 V 3 A 1 5 V 3 A l i n e v o l t a g e p r i m a r y c i r c u i t s M P S s e n s e ...

Страница 243: ... removed An over temperature sensor in the main power supply is used to turn off output voltages if the temperature exceeds the acceptable limit for example if the cooling fan of the instrument fails To reset the main power supply to normal operating conditions turn the instrument off wait until it is approximately at ambient temperature and turn the instrument on again The following table gives t...

Страница 244: ...244 Theory of Operation The Main Power Supply Assembly ...

Страница 245: ...8 8 Introduction to the Control Module Introduction to the screens available for operation of the Agilent 1100 Series autosamplers with the control module ...

Страница 246: ...Control Module firmware revision B 01 01 LC Module firmware revision 3 8x NOTE In case the control module s display seems to be frozen hang up due to a communication problem on the CAN bus unplug the control module from the LC module and reconnect ESC Return to previous screen and scroll through top layer views Analysis Settings m Open context sensitive menus i Information help Enter Store changed...

Страница 247: ...lysis screen Screens available from the Analysis screen The Analysis screen This is the wake up screen if the Agilent 1100 Series autosampler or thermostatted autosampler is the only configured Agilent 1100 module It is used to enter the most common ALS method parameters ...

Страница 248: ...he Control Module Screens available from the Analysis screen The m key allows access to the context sensitive menu Setup view leads you to add sections for additional Agilent 1100 modules Restart re boots the control module ...

Страница 249: ...splay as well The number of parameters on the display are restricted as additional modules are added A maximum of 4 modules are shown automatically If more modules are in the system you have to chose in Setup view The number of parameters on the display are restricted as additional modules are added The m key gives access to a context sensitive menu e g when the cursor is positioned in the excitat...

Страница 250: ...Settings key you open a pull down menu where you can select the ALS module Settings Within the Settings you can change the ALS parameters and with a different set of parameters available through the F1 5 keys F7 key resets the ALS to default values F8 opens a window to turn on the thermostat ...

Страница 251: ... m key for the context sensitive menu The Status command pulls up a monitor screen displaying details of injections and the sample tray Reset will initialize the autosampler Use F1 key More opens a pull down menu Selecting More and Settings pull down menu you can enter special ALS setpoints ...

Страница 252: ...pull down menu you can enter different optimization modes Settings Timetable With the F2 key Timetable you can list the timetable for the ALS Press F7 key Insert to add entries or F6 key Delete to remove entries Press the F7 key Insert to add timetable events Use the F6 key Done to view the entered lines of the timetable ...

Страница 253: ...y for the context sensitive menu It gives you additional tools for the timetable Settings Injection Program With the F3 key Inj Program you can change the settings for the analog outputs Press the F7 key Insert to add program events Use the F6 key Done to view the entered lines of the timetable ...

Страница 254: ...nsitive menu It gives you additional tools for the timetable The F8 key Validate will check the injector program for errors Settings Thermostat With the F4 key Thermostat you can set the autosampler thermostat temperature Settings Run times With the F5 key Runtimes you can change the stop time and the post run time ...

Страница 255: ...ilent 1100 ALS is configured stand alone Signal plot Press F6 key Plot to enter the plot screen available also from the Analysis and System screen Here you can observe the on line signal s To add additional on line signals maximum 3 press F6 key Select If more than one signal is configured see next use the 1 2 3 number key to switch between the signals ...

Страница 256: ...u Press F6 key Select Here you can add additional on line signals maximum are 3 Additional signals could be also pressure or temperature signals from other modules Use the Right Left arrows to switch between Available and Selected Signals Use the F8 key Move to enter available signals into the box for selected signals or vice versa ...

Страница 257: ...method in the mdodule s The PC Card key is only active when a PCMCIA card is inserted in the control module Use F2 key PC Card to save a method on a PCMCIA card Use the Right Left arrows to switch between PC Card and Instrument window Use the UP Down arrows to select the method Use the F7 F8 keys Copy to enter available signals into the box for selected signals or vice versa Press the m key to ope...

Страница 258: ...ivities in the system System Control Use the F1 key Control to select the Autosampler Here you receive information about the not ready conditions if needed F2 key Reset does a re initialization of the ALS Use the F8 key On Off to turn on the thermostat The F1 key Release Vial releases a vial held in the gripper the F2 key Home Gripper returns the gripper to the home position and the F3 key Park Gr...

Страница 259: ... Esc key to receive Views on the F5 key Choose System from the pull down menu Use the F2 key Configure to select the ALS Here you define further special setpoints for the ALS operation Use the F1 key Interfaces to access the interface settings if required Use the F2 key Trays to view the detected sample trays ...

Страница 260: ...receive Views on the F5 key Choose System from the pull down menu Use the F4 key Records to select the ALS Errors are reported either into the System Log F2 or Error Log F3 System Error Log Use the F2 key System Log or F3 key Error Log to look for errors For troubleshooting reasons they can be printed or saved to a file on the PCMCIA card pressing the m key ...

Страница 261: ...rly Maintenance Feedback Use the F1 key EMF to set EMF parameters Choose menu item 1 Setup limits to select the number of injection valve cycles or number of needle movements into the needle seat at which you want to receive a warning If a set limit has been exceeded a message box will pop up This will not stop a sequence or run the information is intended only for planning of maintenance activiti...

Страница 262: ...om the PCMCIA card RESnnnn DLB and press execute If you want to update the main firmware press F7 key Transfer to turn the module into the resident mode LED on module should blink yellow Use the Esc key to receive Views on the F5 key Choose System from the pull down menu Use the F3 key Records to select the ALS Use the F5 key FW Update to enter the Update section Select the a file from the PCMCIA ...

Страница 263: ... screen Maintenance activities Use the Esc key to receive Views on the F5 key Choose System from the pull down menu Use the F3 key Records to select the ALS Use the F4 key Maint log to view and edit the maintenance logbook Use the F7 key Add to add new maintenance activities ...

Страница 264: ...ews on the F5 key Choose System from the pull down menu Use the F3 key Tests to select the ALS Several tests are available to test the Agilent 1100 autosampler Additional test are listed in the function box Press the m key to open the context sensitive menu Menu item 3 Schematics will popup the ALS and ALS thermostat diagram ...

Страница 265: ...265 Introduction to the Control Module Diagnostics and Tests ...

Страница 266: ...266 Introduction to the Control Module Diagnostics and Tests Tray and Transport Alignment Use the F1 key Align to align the autosampler tray or transport ...

Страница 267: ...ton and Arm Use the F2 key Change to start the maintenance procedures for changing the autosampler needle piston and gripper arm Signals Use the F8 key Signals to display the signals available from the ALS thermostat Press F6 key Setup Here you can select which signals are shown ...

Страница 268: ...268 Introduction to the Control Module Diagnostics and Tests ...

Страница 269: ...9 9 Specifications Performance specifications of the Agilent 1100 Series Standard Micro and Preparative Autosamplers ...

Страница 270: ...handling low voltages in maintenance areas error detection and display Injection range 0 1 100 µl in 0 1 µl increments Up to 1500 µl with multiple draw hardware modification required Replicate injections 1 99 from one vial Precision Typically 0 5 RSD of peak areas from 5 100 µl Typically 1 RSD of peak areas from 1 5 µl Minimum sample volume 1 µl from 5 µl sample in 100 µl microvial or 1 µl from 10...

Страница 271: ...g low voltages in maintenance areas error detection and display Injection range 0 1 900 µl in 0 1 µl increments recommended 1 µl increments Up to 1800 µl with multiple draw hardware modification required Replicate injections 1 99 from one vial Precision Typically 0 5 RSD of peak areas from 5 2000 µl Typically 1 RSD of peak areas from 2000 5000 µl Typically 3 RSD of peak areas from 1 5 µl Minimum s...

Страница 272: ... microvials Carryover Typically 0 1 without automated needle wash Typically 0 05 with external needle cleaning and 1 µl injection volume Sample viscosity range 0 2 5 cp Recommended pH range 1 0 8 5 solvent with pH 2 3 should not contain acids which attack stainless steel Upper pH range is limited by fused silica capillaries Material in contact with solvent Stainless steel sapphire PTFE PEEK fused ...

Страница 273: ...areas error detection and display Injection range 0 1 900 µl in 0 1 µl increments recommended 1 µl increments Up to 1800 µl with multiple draw hardware modification required Up to 5000 µl with multiple draw hardware modification required Replicate injections 1 99 from one vial Precision Typically 0 5 RSD of peak areas from 5 2000 µl Typically 1 RSD of peak areas from 2000 5000 µl Typically 3 RSD o...

Страница 274: ...274 Specifications Performance Specifications ...

Страница 275: ...10 Legal Safety and Warranty Information ...

Страница 276: ... be uninterrupted or error free Limitation of Warranty Onsite warranty services are provided at the initial installation point Installation and onsite warranty services are available only in Agilent service travel areas and only in the country of initial purchase unless buyer pays Agilent international prices for the product and services Warranties requiring return to Agilent are not limited to th...

Страница 277: ... PROFITS WHETHER BASED ON CONTRACT TORT OR ANY OTHER LEGAL THEORY Responsibilities of the Customer The customer shall provide 1 access to the products during the specified periods of coverage to perform maintenance 2 adequate working space around the products for servicing by Agilent personnel 3 access to and use of all information and facilities determined necessary by Agilent to service and or m...

Страница 278: ...n accordance with the law of your country Please consult your local Agilent office for the period of the warranty for shipping instructions and for the applicable wording of the local warranty Warranty services are included as specified for Analytical products and options purchased concurrently provided customer is located within a Agilent defined travel area Agilent warranty service provides for ...

Страница 279: ...erating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instru...

Страница 280: ...ument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting Safety Symbols Table 67 shows safety symbols used on the instrument and in the manuals Table 67 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer...

Страница 281: ... damage to the equipment Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A caution alerts you to situations that could cause a possible loss of data Do not proceed beyond a caution until you have fully understood and met the indicated conditions ...

Страница 282: ...s not allowed Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries WARNING Lithiumbatteri Eksplosionsfare ved fejlagtig håndtering Udskiftning må kun ske med batteri af samme fabrikat og type Lever det brugte batteri tilbage til leverandøren WARNING Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt ...

Страница 283: ...d for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position 70 dB Sound Pressur...

Страница 284: ...ch are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCl3 O2 2COCl2 2HCl This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropyleth...

Страница 285: ...s on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the Agilent 1100 series modules for download ...

Страница 286: ...286 Agilent Technologies on Internet ...

Страница 287: ...screens 250 Signal Plot screens 255 Status screens 255 System screen 258 Tests and Diagnostic screens 264 Thermostat screen 254 Timetable screens 252 D damaged packaging 17 delay 38 delay volume 35 38 delivery checklist 17 DRAW 46 draw speed 46 E EJECT 46 eject speed 46 electronics 218 ASIC 220 ASM board 220 battery 221 electronic fuses 221 fan drive 221 firmware 226 sample transport control 221 s...

Страница 288: ...ted autosampler accessory kit contents 20 microswitches 219 missing parts 17 multi draw option 202 N needle drive 207 208 numbering of vials 32 O optimizing performance automated needle wash 46 bypassing the autosampler 35 delay volume 46 delay volume adjustment 46 early maintenan 46 inject valve seal 46 low volume capillary kit 35 46 minimizing delay volume 35 38 P park arm 84 park transport asse...

Страница 289: ...ings 238 S safety information on lithium batteries 282 sample trays 32 numbering of vial positions 33 sampling sequence 204 sampling unit 207 seals metering seal 165 167 sensors 219 shipping 17 35 84 simple repairs 107 site requirements 14 specifications 16 17 270 stack configuration 23 25 rear view 23 25 stator 209 status indicator 50 status indicators 51 step commands 89 step functions 50 steppe...

Страница 290: ...290 Index ...

Страница 291: ......

Страница 292: ...tatted Micro and and Preparative Autosamplers The manual describes the following installing the autosamplers optimizing performance troubleshooting and test functions repairing the autosamplers parts and materials introduction to the autosamplers screens of the local control module specifications safety and warranty ...

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