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Advanced

®

Osmometer Model 3300 User’s Guide

48

2. Knows of no defects in design or mate-

rials which may cause bodily injury.

3. Will endeavor to advise the user of

changes or improvements in the instru-
ment as they are developed, so that the
user may take steps to improve the
safety and performance of his equip-
ment throughout its useful life.

4. Will replace or repair equipment

according to the guarantee on the
attached warranty.

5. Will cooperate closely in common

defense of any accident involving this
equipment, or third-party suit against
the user or operating personnel, if
advised immediately by the user of the
occurrence of any accident.

THE USER WARRANTS THAT:

1. The instrument will be used reason-

ably.

2. The instrument will be regularly main-

tained according to this manual,
including a log of all service, tests and
repairs performed on the equipment,
and records will be kept of all requests
for repair made to Advanced
Instruments where such repairs were
beyond the ability of local service per-
sonnel.

3. The instrument will not be altered

without written approval from
Advanced Instruments.

4. Advanced Instruments will be notified

immediately if any injury occurs in any
association with the instrument and will
be allowed prompt and thorough exami-

nation of the instrument in question.

5. Advanced Instruments will be held

harmless in cases of injury arising (see
definitions below):

a. Beyond the useful life of the

equipment.

b. From unreasonable use.

c. When Advanced Instruments is

not immediately notified of said
injury.

d. From interpretation of results.

DEFINITIONS

1. 

"

Useful life

"

is:

a. The same as the depreciation life in

the Internal Revenue Service guide-
lines, whether or not the user actual-
ly depreciates the instrument, but not
to exceed 10 years from date of
delivery to the user.

b. Only during the time the equipment

has been maintained on a regular
basis as prescribed by Advanced
Instruments.  If the user is in an
area which has no local service,
Advanced Instruments may require
a local service person (understood
to mean the person actually per-
forming the "hands-on" service of
the equipment) to attend and pass a
reasonable maintenance and repair
course.

c. Only during the time when the user

has not altered the equipment in
any way without written approval
from Advanced Instruments.

Содержание 3300

Страница 1: ...The Advanced Micro Osmometer Model 3300 User s Guide 3305 Rev9 012303 ...

Страница 2: ...dvanced Instruments has reviewed this guide thoroughly All material contained within is believed reliable but the accuracy and completeness are not guaranteed or warranted and are not intended to be representations or warranties concerning the product described Hot Line Service If you have any questions or problems regarding the proper operation of your instrument please contact our Hot Line Servi...

Страница 3: ...ent 1 Step 3 Obtain additional items 2 Step 4 Power up your instrument 2 Step 5 Set your date and time 3 Step 6 Check your initial calibration 3 Step 7 Proceed to instrument operation 4 Chapter 2 Instrument Operation 5 Hazardous material cautions 5 Function of major components 5 Sample handling 10 Standards controls 10 Sample test procedure 11 Sample test errors 12 Recall results 13 Repeatability ...

Страница 4: ...ice maintainance cautions 25 Obtaining service 27 Troubleshooting checks 28 Internal diagnostics 28 Fuse replacement 33 Chamber cleaning 34 Sample plunger replacement and calibration 34 Appendices Appendix A Troubleshooting Table 37 Appendix B Product Specifications 43 Appendix C Regulatory Notices 45 Appendix D Warranty and warranty duties 47 Appendix E Supplemental RS 232 information 51 Appendix...

Страница 5: ... Sample Test Kit 500 tests Sampler Plunger Wires 2 Thermal Printer with Interface Cable Operation Manual Thermal Paper Roll and Printer Power Supply Note Contact customer service to order the appropriate power supply Printer Paper 5 rolls User s Guide Service Manual Serial Cable Software Upgrade Barcode Scanner PART NO 3M0825 330700A 3MA800 3M0828 330555 3D3835 3305 3305SM RS232 Cable 330990 33001...

Страница 6: ...vi Notes ...

Страница 7: ...s US 781 320 9000 elsewhere 781 320 3669 fax DESCRIPTION ClinitrolTM Reference Solution 10 2 mL ampules Protinol 3 Level Protein Control Kit 9 3 mL bottles 3 of each level 5 Value Osmolality Linearity Set 10 2 mL ampules 2 of each value 50 mOsm kg Calibration Standard 10 2 mL ampules 850 mOsm kg Calibration Standard 10 2 mL ampules PART NO 3MA029 3MA028 3LA028 3MA005 3MA085 ...

Страница 8: ...viii Notes ...

Страница 9: ...nt within an equilateral triangle is intended to alert the user to the presence of important oper ating and maintenance servicing instructions in the litera ture accompanying this product The lightning flash with arrowhead symbol within an equi lateral triangle is intended to alert the user to the presence of uninsulated dangerous voltage within the product s enclosure that may be of sufficient ma...

Страница 10: ...ngs marked on the product s electrical ratings label The cord end that connects to the product must have an IEC 60320 connector Plug the product into an approved grounded electrical outlet Do not disable the power cord s grounding plug If an extension cord or power strip is used make sure that the cord or strip is rated for the product and that the total ampere ratings of all products plugged into...

Страница 11: ...ed the speci fied operating environment temperatures Never use this product in a wet area To avoid injury or fire hazard do not operate this product in an explosive atmosphere Do not install or use the product on an unstable non level work sur face Do not operate this product with the covers removed or unsecured xi Safe Use ...

Страница 12: ...xii Advanced Osmometer Model 3300 User s Guide Notes ...

Страница 13: ...entration in other words the number of particles in solution Of the colligative properties measurement of the freezing point allows the concentration of an aqueous solution to be easily determined with great precision The freezing point of pure H2O is precisely 0 010 C One mole of a non dissociating solute such as glucose where the solute does not dissociate into ionic species but remains intact w...

Страница 14: ...y determined Each of the colligative properties has a similar problem and though each of the colligative properties changes in direct proportion to the solute concentration each requires a different mode unit of measure ment Osmolality is a common unit of concentration measurement that can be used to relate all the colligative properties to each other and to other concentration units Because of it...

Страница 15: ...ssued to Augustus Fiske The time over which liquid solid equilibrium develops and is main tained is a function of the speed with which the heat of fusion is liber ated vs the speed it is transferred away or absorbed by the surrounding environment This ratio can be slowed and the equilibrium time stretched to give a distinct plateau height measurable to 0 001 C Sensitive thermistor probes monitor t...

Страница 16: ...logram of pure solvent Osmolality Osmols of solute particles per kilogram of pure sol vent As noted above most ionic solutes do not completely dis sociate Osmolality is a unit of concentration that takes into account the dissociative effect Osmolality is usually expressed in mOsm kg H2O One milliosmol mOsm is 10 3 osmols Osmolality is defined as where ø osmotic coefficient which accounts for the d...

Страница 17: ...ram of water depresses the freezing point by 1 858 millidegrees Celsius m C Supercooling The tendency of a substance to remain in the liquid state when cooled below its freezing point Crystallization Temperature Aqueous solutions can be induced to freeze i e crystallize most reliably when supercooled When super cooled agitating the solution freeze pulse induces crystal formation The crystallizatio...

Страница 18: ...xviii Advanced Osmometer Model 3300 User s Guide Notes ...

Страница 19: ...ecially near the front of the instrument where proper ventilation is needed Electric outlet availability Your instrument will need to operate within five feet of a properly grounded three prong electrical outlet capable of continuously supplying 1 ampere at 200 250V to 1 25 amperes at 100 130V If the instrument is not grounded properly its operation may be impaired and a safety hazard may exist Th...

Страница 20: ...ents immediately for repair or replacement e Fill out the postage paid U S A Only warranty card enclosed Mark the appropriate boxes if you wish to receive additional informa tion Customers outside of the United States may fax the warranty card to 781 320 8181 Step 3 Obtain additional items Soft no lint non ionic paper tissues are needed for wiping the sample cells prior to testing Please be sure t...

Страница 21: ...date and time If you wish you may now change your date and time settings by using the instructions found on page 13 of this user s guide Step 6 Check your initial calibration Your instrument has been carefully calibrated by the manufacturer but to verify that this calibration is accurate within your operating environ ment run tests on the 290 mOsm kg reference solution and or Protinol biological c...

Страница 22: ...n this chapter your instrument is ready for use To learn how to operate your instrument read the next chapter Instrument Operation We strongly recommend that you read the entire second chapter before attempting to operate your osmometer Advanced Osmometer Model 3300 User s Guide 4 ...

Страница 23: ...s spilled on or inside the equip ment the user is responsible for taking the appropriate decontamination steps as outlined by the user s national safety council or equivalent agency for hazardous materi als and those policies and procedures established within the user s place of business To avoid injury or fire hazard do not operate this product in an explosive atmosphere Function of major compone...

Страница 24: ...onfiguration More in depth technical details are available in the unit service manual sold separately Processor Board The processor board is the smaller of the two printed circuit boards and contains the Intel 80C186EB central processor two flash EPROM s RAM Realtime clock watchdog circuit and glue logic 6 Figure 1 Model 3300 Components and Controls Display Panel Multifunction Soft keys Operating ...

Страница 25: ... the sampler to the freezing chamber Cooling chamber measurement and control circuits A high reliability thermoelectric module controls cooling chamber tem perature while a thermistor sample probe measures the dynamic tem perature of a sample Test results based on the freezing point of the sample are automatically sent to the instrument s display Display panel Figure 1 The display panel displays T...

Страница 26: ... printer port on the rear panel allows you to use a printer in conjunc tion with this instrument For instructions on using a printer with this instrument see Using a printer with the Model 3300 in this chapter RS 232 port Figure 2 The RS 232 port allows you to output your instrument s data messages to an external device such as a computer and to upgrade your instrument software For more detailed i...

Страница 27: ...ervisor operator keyswitch Figure 2 The supervisor operator keyswitch allows certain instrument functions to be locked The Supervisor keyswitch position is required for re calibration and for access to the following diagnostic menu items Set Probe Bin s Set Serial Rate Serial Comm Set and Select Language If a restricted function is attempted while Supervisor Operator keyswitch is in the operator p...

Страница 28: ...ls Advanced Instruments offers and recommends the use of specific stan dards and controls with your Model 3300 osmometer Each type may be used to assess a different aspect of your instrument s performance For information on obtaining these standards and controls contact Advanced Instruments or an authorized representative Clinitrol 290 Reference Solution Part No 3MA029 We recommend that you run sa...

Страница 29: ... careful not to crack the sample tip 2 Depress the sampler s plunger and insert the sample tip at least inch 6 mm below the surface of the fluid to be tested Gently release the plunger to load a 20 µL sample 3 Visually inspect the sample If there are any large voids or bubbles in the sample expel the sample and load a bubble free sample 4 Blot the sides of the loaded sample cell with a soft no lin...

Страница 30: ...dry chamber cleaner into the sample port and rotate it four or five times in both a clockwise and counterclockwise direc tion Withdraw chamber cleaner and insert the opposite end Rotate the chamber cleaner in the same manner and leave it in the sample port until your next test 11 Remove the used sample tip from the sampler by pressing firmly enough on the sampler plunger to dislodge the tip Discar...

Страница 31: ...eat all samples as well as standards and reference solutions uni formly before the test Microsamples are more susceptible to contamination and evapora tion than larger samples Avoid leaving sample containers open Cold samples are susceptible to condensation warmer samples are susceptible to evaporation Use only the Advanced Model 3300 sampling system Each sys tem comes with specific operating inst...

Страница 32: ...elect they will be filtered out of the calculations To use this option follow this procedure 1 At Osmometer Ready the two multifunction soft keys are labeled CALIB and DIAG respectively Press the soft key labeled DIAG to enter the diagnostic test menu 2 While you are within the diagnostic test menu the soft key labels will change to START and INDEX Use the INDEX soft key to cycle through your choi...

Страница 33: ...ively Press the soft key labeled DIAG to enter the diagnostic test menu 2 While you are within the diagnostic test menu the soft key labels will change to START and INDEX Use the INDEX soft key to cycle through your choices until you see 13 Set Date Time 3 Press START to enter the Set Date Time mode Your instrument will display the current date and time 4 Press the left soft key to select the date...

Страница 34: ... following steps 1 At Osmometer Ready the two multifunction soft keys are labeled CALIB and DIAG respectively Press the soft key labeled DIAG to enter the diagnostic test menu 2 While you are within the diagnostic test menu the soft key labels will change to START and INDEX Use the INDEX soft key to cycle through your choices until you see 3 Set Probe Bin s 3 Press START to enter the selection men...

Страница 35: ... are within the diagnostic test menu the soft key labels will change to START and INDEX Use the INDEX soft key to cycle through your choices until you see 12 Data Capture 3 Press START to enter the selection menu 4 Select the soft key for the mode you want to use 5 Press the two soft keys simultaneously to lock in your selection 6 Press the two soft keys again simultaneously to return to Osmometer...

Страница 36: ...barcode scanner must be programmed as follows referring to the scanner users guide 10 msec Intercharacter Delay Disable Line Feed Suffix No Parity 1200 BAUD Rate 8 Data Bits When the barcode scanner part 330016 is connected to the Model 3300 the instrument operator may scan an identifying barcode before introducing a sample NOTE The barcode configuration may work with many different scanners but n...

Страница 37: ...her than supplied by Advanced Instruments may result in excessive electromagnetic noise and unintended interference with other devices Using the Model 3300 s RS 232 port The RS 232 port allows you to output to an external device such as a computer and to update your instrument software Almost every item of information displayed by your instrument is also transmitted over the RS 232 port including ...

Страница 38: ... 232 port Such software updates are made available as new features and other improvements are added during the life cycle of the instrument For information on the availability of software updates contact Advanced Instruments Inc or an authorized distributor See Chapter 4 page 23 For directions on performing the RS 232 serial port software upgrade consult the docu mentation supplied with the upgrad...

Страница 39: ...libration points the instrument automatically performs internal calibration The calibration procedure is as follows Note that maintaining and loading accurate uncontaminated standard samples is extremely important 1 At Osmometer Ready press the left soft key labeled CALIB to initiate the calibration process The calibration program will prompt you to run a sample of the 50 mOsm calibration standard...

Страница 40: ...mples at either calibration level the calibration procedure has failed and you should carefully repeat the procedure If you experience Calibration Not Complete more than once you have a repeatability problem and should consult Chapter 4 for troubleshooting and service information Calibration notes The Model 3300 will retain its previous calibration data until it com pletes a re calibration and the...

Страница 41: ...lay Test Canceled beep once then display Calib Canceled and beep again The existing calibration will not be affected If you accidentally press a function soft key during a series of regu lar tests the same procedure will terminate the calibrate mode with out affecting the calibration and normal testing may be continued 23 Calibration ...

Страница 42: ...Advanced Osmometer Model 3300 User s Guide 24 Notes ...

Страница 43: ...shock exces sive temperatures or mechanical hazards Performing service or maintenance not detailed in the User s Guide with or without a Service Manual should only be done by a qualified service technician Never restrict airflow into or out of the product Occasion ally check the air vents for blockage Wipe the exterior of the product with a soft damp cloth as needed Using cleaning products other t...

Страница 44: ...equipped with operator accessible fuses If a fuse blows it may be due to a power surge or failure of a component Replace the fuse only once If the fuse blows a second time it is probably caused by failure of a component part If this occurs refer service to qualified service personnel Always replace the fuse with one of the same rating voltage and type Never replace the fuse with one of a higher cu...

Страница 45: ...roblem ready You should use a telephone as close to your instrument as possible to facili tate making recommended diagnostic checks If you need to order parts or service a purchase order from your purchasing agent will be neces sary After Hot Line diagnosis your service technician may assist you in making minor repairs over the phone dispatch a local service represen tative or have you ship your i...

Страница 46: ...ument is designed in such a way that any incomplete task will be associated with an error message many self explanatory that will help you discover the source of your problem You can find all error messages and descriptions of what they mean in Appendix B Internal diagnostics The diagnostic menu allows any of a series of tests to be run to check one functional subsystem of the instrument or to per...

Страница 47: ...to test the block probe the sample cooling assembly or the sample probe On entry the A D tests display the target sample cooling block tempera ture the channel being read and the current channel reading or duty cycle The display is in the form off Blk NNNN NN ohm where off indicates that a target temperature has not yet been selected Blk indicates that the block probe channel is being tested NNNN ...

Страница 48: ...ure to run the test 1 On entry the probe bin test will display Probe Bin Test YES Ready CANCEL 2 Press YES to start the test When YES is pressed the display changes to Insert 50 mOsm Sample CANCEL 3 To continue load a sample from a freshly opened ampule of Advanced 50 mOsm standard and run the test using the sample test procedure detailed in Chapter 2 4 Cooling Sample is displayed as in a normal t...

Страница 49: ...of the scanner for your symbologies 3 Press CANCEL or both keys to exit to the test menu Display Print Test This menu item is a simple check of the display serial port and printer if connected On entry a single line of characters will be displayed and sent to the serial and parallel ports The characters displayed have been chosen to exercise every dot in the character matrix This makes it possible...

Страница 50: ...ey to indicate proper operation Event Record On entry this menu item enables downloading the internally stored event record to both the serial and parallel ports When ready press YES to download Press CANCEL or both soft keys and release them simultaneously to exit to the menu Toggle the INDEX soft key to select another menu item or press both soft keys and release them simultaneously to exit from...

Страница 51: ...hould start up as normal Chamber cleaning The cooling chamber and probe are easy to keep clean and dry by faith fully following the operating instructions for cleaning the freezing chamber after each test If traces of standards or biological samples are left in the sample chamber however the task will be more difficult and damp cleaning will probably be required Two indicators that damp cleaning m...

Страница 52: ...er tip of the sampler every 500 tests or every package of sample cells To replace the plunger tip use the following procedure 1 Unscrew the calibration gauge and key 2 Rotate the sampler shaft until the calibration setscrew appears beneath the access hole in the side of the sampler body 3 Place the key end of the calibration gauge in the access hole and turn counter clockwise to loosen the setscre...

Страница 53: ...ollowing procedure 1 Place a new sample tip on the sampler 2 Unscrew the calibration gauge and key 4 Insert the key end of the calibration gauge into the sample tip 5 Visually inspect the posi tion of the end of the sampler plunger tip and the end of the calibration key There should be no gap between the two 6 If necessary reset the sampler plunger wire as described above 35 Figure 5 Sample plunge...

Страница 54: ...36 Advanced Osmometer Model 3300 User s Guide Notes ...

Страница 55: ...problem with the internal switch that initiates the test Try turning the instrument on and then off If the problem persists please consult an Advanced Instruments Model 3300 Service Manual or contact Advanced Instruments for Hot Line Service Most often poor repeatability comes as a result of poor technique or not following procedure Be sure all sample tips are clean as well as the sampler itself I...

Страница 56: ...icates that your cooling system is not functioning properly Contact Advanced Instruments for Hot Line Service This message indicates that the event record stored in memory has been corrupted Try restarting your instrument If this does not solve your problem Contact Advanced Instruments to obtain Hot Line Service Try restarting your instrument If this does not solve your problem contact Advanced In...

Страница 57: ...u that a new software version has been installed since you last powered the instrument or that the information stored in parameter RAM has been corrupted The instrument will reload factory defaults This message indicates that you need to re calibrate your instrument Re calibrate close ly following the instructions in Chapter 3 If the error message repeats after successful re calibration obtain Hot...

Страница 58: ...pre freeze message usually appears as a result of an unclean freezing chamber Clean your chamber as described in Chapter 4 and be sure that the chamber is cleaned after each test If this does not solve your problem you should check the probe bin number and obtain service if nec essary Switch the instrument off then on Check the sample probe by running the A D tests If the error message does not pe...

Страница 59: ...ecessary the block and probe bin numbers If you heard no impact the problem most likely lies with the solenoid impactor Retry the test and if necessary contact Advanced Instruments for Hot Line Service This message indicates a problem with your solenoid impactor Try restarting your instrument If this does not solve your prob lem contact Advanced Instruments for Hot Line Service This message will a...

Страница 60: ... instrument If this does not solve your prob lem contact Advanced Instruments for Hot Line Service This message indicates that your instrument was unable to complete the test in the allot ted time Be sure that your operator tech nique is sound and retry the test If the problem persists check your block probe number If you need more assistance call for Hot Line Service ...

Страница 61: ... VAC 100 Watts Integral lithium cell two years minimum 20 µL Single sample 2 line digital display mOsm kg H2O 0 to 2000 mOsm kg H2O Within 1 of straight line between calibration points of 50 and 850 mOsm Within 1 5 above 850 mOsm 1 mOsm kg H2O 2 mOsm kg H2O 1 S D between 0 and 400 mOsm kg H20 0 5 1 S D between 400 and 2000 mOsm kg H2O DTE EIA 232 V 24 RS 232 parallel printer and optional barcode s...

Страница 62: ...h Height Weight Net Shipping Warranty Certification 5 to 80 E H non condensing 30 seconds from power on 60 seconds per sample inches centimeters 10 5 27 13 33 15 5 39 pounds kilograms 16 7 3 25 11 4 One year limited warranty on workmanship and all parts except glass plastic and parts warranted by their makers ...

Страница 63: ...onal or international approvals to this product Consult the distributor or dealer for more information and documentation Connections to this product must be made with shielded cables Use of non shielded cables may violate RFI EMI limits Symbol Conventions This symbol indicates conformity to relevant European directives This symbol indicates the product was tested to conform to relevant Canadian an...

Страница 64: ...of This product meets the intent of Directive 89 336 EEC Conformity EMC for Electromagnetic Compatibility Compliance was demonstrated using the following standards as listed in the Official Journal of the European Communities Consult the Declaration of Conformance certificate shipped with the product for the latest update EN 61326 1998 Group 1 Class B Electrical Equipment for Measurement Control a...

Страница 65: ...onsibilities which constitute contractual warranties and conditions between the seller and the user for the maximum benefit and useful ness of the instrument ADVANCED INSTRUMENTS WARRANTS THAT IT 1 Has produced equipment equal to or exceeding that of any competitive product in the same price range in standards of design material and workmanship ...

Страница 66: ...without written approval from Advanced Instruments 4 Advanced Instruments will be notified immediately if any injury occurs in any association with the instrument and will be allowed prompt and thorough exami nation of the instrument in question 5 Advanced Instruments will be held harmless in cases of injury arising see definitions below a Beyond the useful life of the equipment b From unreasonabl...

Страница 67: ...corrected repairs d Only for the purpose stated in the instructions provided with the instrument e In which the equipment has been maintained according to the instructions provided 3 Immediate notification is a Recognition that time is of the essence when any accident mal practice or product liability arises which involves Advanced Instruments equipment b Notification to Advanced Instruments immed...

Страница 68: ...Advanced Osmometer Model 3300 User s Guide 50 Notes ...

Страница 69: ...eturn 0D Hex Line Feed 0A Hex Note that your instrument is only capable of outputting information At this time there is no protocol for talking to the instru ment Sample RS 232 Setup As a typical example of a communications program setup the following instructions will describe the process necessary for using your instru ment in conjunction with Hyperterminal for Windows 95 and a null modem cable ...

Страница 70: ...ngs menu 6 Chose TTY for the Emulation option 7 Select ASCII setup 8 Set the Sending option to Echo Typed Characters Locally 9 Set the Receiving option to Wrap Lines that Exceed Terminal Width You should at this point see all instrument output in the Hyperterminal window of your computer screen Advanced Osmometer Model 3300 User s Guide 52 Null Modem Cable Shield connects to metalized connector ho...

Страница 71: ...Model Model 3300 Serial Number Software Revision Sample Probe Number Block Probe Number 53 Service Log Appendix F Date Problem Symptom Action ...

Страница 72: ...Advanced Osmometer Model 3300 User s Guide 54 Date Problem Symptom Action ...

Страница 73: ...0 crystallization temperature xvii D data capture 17 date 15 diagnostic test menu 13 diagnostics 28 display print test 31 E error messages 28 37 errors 12 event record 32 F freezing point xiii xv freezing point depression xiii freezing point plateau xvii fuse replacement 32 fuses 8 28 H heat of fusion xv xvii Hyperterminal 51 I installation 1 K key test 32 L language 17 linearity set 11 M melting ...

Страница 74: ... repeatability 13 RS 232 port 8 19 51 S sample freeze error 40 sample plunger 34 sample pre freeze 40 sample preparation 10 sample probe 7 sample test procedure 11 sampler 7 select language 17 service 25 service log 53 set date time 15 set probe bin s 16 settings 15 solenoid test 32 specifications 37 standards 10 statistics 14 supercooling xiv xvii supervisor operator keyswitch 9 T test time out e...

Страница 75: ... Chapter Name ...

Страница 76: ... Advanced Osmometer Model 3300 User s Guide ...

Страница 77: ... Chapter Name ...

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