3Brain BioCAM X, Руководство пользователя

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BioChip with dirty surface
Symptoms
This scenario can occur when testing a BioChip with no biological preparation. The chip might not have
been properly cleaned or has not been used for a long period. In this situation, several electrodes on
the array could exhibit either higher level of noise or signals with fake peaks. In Brainwave, you typically
see several spots (single electrodes) with red values on the overall array (left image) . Single electrode
raw traces can have either high noise levels (centre image: bottom traces are more noisy than average
traces on top) or peaks at a sustained pace (right image). Most of the peaks have a shape quite different
from that of a real spike, but some might still look similar.
Causes
BioChip CMOS surface is dirty, which makes some electrodes unstable.
Possible scenarios and remedies
# Scenario Remedy
S9 BioChip electrodes are dirty of debris and/or
more likely of dust particles. While the chip is connected and is filled with
saline solution, start acquiring and visualizing
incoming data with BrainWave to have a feed-
back on whether the problem is solved.
As a first step, try pipetting with a small plastic
pipette by placing the tip roughly at 45° and
very close to the CMOS central area.
Be very
careful not to touch the CMOS with the tip .
If this does not help, you can also try to use
a soft brush directly on the CMOS surface by
gently brushing repeatedly until all the instable
electrodes get recovered.