Molecular Devices GenePix 4300A User Manual Download | Manualshive

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GenePix

®

4300A/4400A

High-Resolution Microarray Scanner

User Guide

0112-0173 B

October 2010

GenePix.book Page 1 Friday, October 22, 2010 2:59 PM

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Summary of Contents for GenePix 4300A

Page 1: ...GenePix 4300A 4400A High Resolution Microarray Scanner User Guide 0112 0173 B October 2010 GenePix book Page 1 Friday October 22 2010 2 59 PM...

Page 2: ...h usage is intended only to designate those manufacturers products as supplied by Molecular Devices for incorporation into its equipment and does not imply any right and or license to use or permit ot...

Page 3: ...THAT HAVE INTELLECTUAL PROPERTY RIGHTS IN THE POTENTIAL FIELD OF APPLICATION OF THIS INSTRUMENT INCLUDE WITHOUT LIMITATION AFFYMETRIX INC AFFYMETRIX AGILENT AND OXFORD GENE TECHNOLOGY THIS INSTRUMENT...

Page 4: ...4 0112 0173 B GenePix book Page 4 Friday October 22 2010 2 59 PM...

Page 5: ...2 PMT 12 Status Lights 13 Scanner Specifications 14 Site Requirements 15 Chapter 2 Installation 17 Unpacking the scanner and positioning it on the bench 17 Installing the Software 20 Turning on the Sc...

Page 6: ...s 6 0112 0173 B Appendix A Warranty and Service 35 Standard Warranty 35 Out of Warranty Repair Service 35 Optional Service Agreement 35 Technical Support 36 GenePix book Page 6 Friday October 22 2010...

Page 7: ...ration that may cause damage to the instrument device or data if the precautions are not followed Related Documentation The customer documentation for the GenePix 4300A 4400A Microarray Scanner includ...

Page 8: ...Foreword 8 0112 0173 B GenePix book Page 8 Friday October 22 2010 2 59 PM...

Page 9: ...hannels 2 5 m resolution for the 4400A in approximately 60 minutes for all four channels Scanner Features Up to four internally installed laser excitation sources Gradient neutral density wheel permit...

Page 10: ...tube Status lights Figure1 1 GenePix 4300A 4400A Microarray Scanner Protective enclosure The protective enclosure protects the user from exposure to laser radiation high voltage and moving parts WARNI...

Page 11: ...luctuations Furthermore each laser has integrated output monitoring feedback to compensate for fluctuations in photon output ensuring consistency in measured fluorescence The scanner can be configured...

Page 12: ...e emission spectrum of the respective dye designed to reject laser light at OD 8 optical density for each respective laser excitation and reject broad spectrum light at OD 5 For specific information o...

Page 13: ...ner indicate status Device standby Scanning Eject Table1 1 Status lights Item Name Description 1 Device standby The scanner is powered up and ready to scan a slide 2 Scanning The slide is in the load...

Page 14: ...output 16 bit Image format Single or multi image TIFF Computer interface USB 2 0 Scan time Four minutes per channel for full size scan area at 10 m resolution Dynamic range Four orders of magnitude Ro...

Page 15: ...100 to 240 VAC 2A max Space 25 6 inches deep by 16 9 inches wide by 13 4 inches high 65 cm by 43 cm by 34 cm An additional 6 inches 15 2 cm for clearance Bench support Sufficient to support 99 lbs 45...

Page 16: ...Introduction 16 0112 0173 B GenePix book Page 16 Friday October 22 2010 2 59 PM...

Page 17: ...air you must return it to Molecular Devices in the original packaging If the crate has been damaged in transit you must retain it for inspection by the carrier WARNING Shock Hazard In an emergency use...

Page 18: ...e scanner warranty 2 Lift the scanner using the molded lifting handgrips and then place the scanner on a flat level surface away from direct sunlight dust drafts vibration and moisture Carefully move...

Page 19: ...anner and the lab s power outlet Molecular Devices recommends you use a surge protector between the mains power cable and the power outlet Tip For proper ventilation and cooling leave at least six inc...

Page 20: ...stall the GenePix Pro Software CAUTION Do not to insert the security key into the control computer until after the GenePix Pro Software has been installed 4 Install the security key For more informati...

Page 21: ...the Hardware Diagnostics Report every two hours during operation Inserting a Slide The slide holder is a precision component designed to ensure proper focusing and field uniformity Improper handling...

Page 22: ...to the scan position Table2 3 Slider holder Ite m Name Description 1 Slide door In raised position 2 Slide holder In load position 3 Slide Place feature side down 4 Barcode Barcode is facing the front...

Page 23: ...er shutdown of the GenePix 4300A 4400A Microarray Scanner ensures the scanner or software are not damaged To shut down the scanner 1 Close the GenePix Pro Software 2 Turn off the scanner power switch...

Page 24: ...Installation 24 0112 0173 B GenePix book Page 24 Friday October 22 2010 2 59 PM...

Page 25: ...rvice from Molecular Devices representatives or before returning the scanner or any components to Molecular Devices WARNING Biohazardous Material Never perform any maintenance procedures on the scanne...

Page 26: ...lder 2 Close the GenePix Pro Software 3 Click Start Programs Molecular Devices GenePix Pro GenePix Utilities The GenePix Utilities window appears 4 Click Park Scanner for Shipping A message box appear...

Page 27: ...r and positioning it on the bench on page 17 Installing Custom Filters You can install twelve custom filters in the filter wheel When choosing filters it is important to match the optical characterist...

Page 28: ...h on the scanner 2 Insert the 5 32 inch Allen wrench into the filter door latch turn the wrench counterclockwise to release the latch and then pull the filter door down Table3 1 Filter door Item Name...

Page 29: ...from the scanner 4 Rotate the filter wheel until an open access port is visible in the top of the filter cartridge Table3 2 Filter cartridge Item Name Description 1 Filter cartridge 2 Filter cartridg...

Page 30: ...port No filter installed in photo 2 Indicator port Displays filter position number 3 Filter cartridge 4 Filter wheel Thumb wheel located on the edge of the filter cartridge 2 1 3 4 Note If using a fil...

Page 31: ...Make sure the filter is seated properly 7 Place the filter ring in the access port with the grooves facing up Table3 4 Filter arrow pointing down into access port Item Name Description 1 Filter Lower...

Page 32: ...9 To install additional filters repeat steps 4 to 8 10 Position the filter cartridge inside the scanner close the filter door and then insert and turn the 5 32 inch Allen wrench clockwise to secure th...

Page 33: ...pears 4 Select the Installed filter position number then type a Formal Name and an Alternate Name Molecular Devices recommends you use the filter supplier s designation as the Formal Name and an easil...

Page 34: ...door is open The GenePix Pro Software does not recognize the scanner or the slide You can no longer hear the distinctive metal on metal sound that the mechanical interlock makes when it falls into pl...

Page 35: ...Products returned to Molecular Devices for repair should be properly packaged with transportation charges prepaid Molecular Devices will pay for the return shipping of the product to the customer If t...

Page 36: ...our name you can then register your instrument If you require advice on the use of your GenePix 4300A 4400A Microarray Scanner do not hesitate to contact Molecular Devices Technical Support Visit the...

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