Leica SP8-X Abridged Instructions Download | Manualshive

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Wellcome Trust Centre for Human Genetics

Cellular-Imaging Microscopy Core

The Leica SP8-X
SMD Confocal

Abridged INSTRUCTIONS

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Summary of Contents for SP8-X

Page 1: ...Page 1 Wellcome Trust Centre for Human Genetics Cellular Imaging Microscopy Core The Leica SP8 X SMD Confocal Abridged INSTRUCTIONS...

Page 2: ...ed scanning XY Stage including Mark Find and Autostitching Mosaic Modules Passive anti vibration microscope air table AOBS filter free scanhead WLL2 tuneable pulsed single diode laser with 200 lines f...

Page 3: ...rst Log in with the same WTCHG account on both PCs In the Leica LSM software if you set the WWL to Constant Percent at say 10 the actual laser power will vary depending on the laser line selected If y...

Page 4: ...ectives never change from an oil to an air objective when focussed on the specimen under oil you can start with the low power air objective and then move up to the hi power oil objective The large HC...

Page 5: ...Page 5...

Page 6: ...when looking down the microscope press the diochroic filter selection button e g DAPI and then shutter Also use the fluorescence transmitted light toggle button on the bottom left side of the microsc...

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Page 8: ...Page 8 SmartMove XYZ Stage Controller...

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Page 10: ...ight Red LED rocker switch Note this controls power to the PicaQuant PC and both PC monitors 8 The Leica SP8 Master PC monitor will switch on and this will start up the PicaQuant Slave PC automaticall...

Page 11: ...the SP8 Master PC first 6 Shut Down the SP8 Master PC Transfer any data files to the network drives first 7 Turn the Laser Emission key to off There s no argon laser to cool down and the White Light L...

Page 12: ...e any microscope imaging problems or queries just ask a member of the Microscopy Core staff for help There is additional help and advice on our Core web pages http www well ox ac uk microscopy WTCHG L...

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