Zeiss LSM800-02 Quick Start Quide Download Page 5

 

 

Tile scan dialog will open 

 

Set desired number of horizontal and vertical tiles 

 

You can choose different regions to scan. Choose regions and press button “+” to add it. 

 

Press “start experiment” 

 

Advanced Set up allows you to do preview scan of region of interest. 
 

 

You can also add multipositions in this tab: 
Important: to make sure that the software images the positions in the order you added them, 
make sure that you DISABLE the feature “tile regions/positions” in the options menu of the 
Tiles bar (see picture on the next page) 
 

 

 

 
 

4.

 

Data Saving: 
 

Save your data to D: data folder. Note that this computer is not backed up. Please move your 
data as soon as to the file server, all the user data will be deleted automatically after one 
month.  
 

Disable this option to keep 

the order of positions during 

imaging! 

Summary of Contents for LSM800-02

Page 1: ...an Apochromat 40x NA 1 2 Water not mounted Laser lines 405 488 561 640 nm Detectors Two very sensitive Gallium arsenide phosphide photomultiplier tube GaAsP PMTs detectors with free choice of the spec...

Page 2: ...the main microscope menu 3 Since this is an upright system changing objectives can only be manually done by rotating the objective revolver Rotation should work very easily when objectives are in the...

Page 3: ...ed dyes are not set to the 1st detector due to the Variable dichroic arrangement If you are using the detector for transmitted light ESID activate ESID represented in the light path scheme Adjust inte...

Page 4: ...ber of pixels and the pixel size according to Nyquist sampling criteria Note More pixel than suggested by the software as optimal rarely make sense On the other hand feel free to decrease the pixel nu...

Page 5: ...s tab Important to make sure that the software images the positions in the order you added them make sure that you DISABLE the feature tile regions positions in the options menu of the Tiles bar see p...

Page 6: ...rked with a home logo as this setting will be used in the next startup of the system and might cause potential damage to GaAsP PMTs 2 Close the ZEN Software 3 Shut down the computer 4 Turn off the coo...

Page 7: ...ser should be avoided at all times Laser safety goggles are situated at all workspaces and should be used in any situation where potential contact of eyes with Laser light of the classes 3B or 4 is po...

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