2
2.
Switch on SYSTEM (2).
3.
Switch on COMPONENTS (3).
4.
Make sure that lasers are on. Laser key should be in position “I”. (4)
5.
Switch on the computer.
6.
Turn on Cool LED (by pressing
Big blue on/off button
), if you need it for visual
examination and don’t forget to close the shutter when you don’t need it or at the end
of your session.
7.
Login: LSMuser (password: Useme!11)
8.
Open
ZEN Blue
Software and press “ZEN System” ->
Restart the ZEN
if it is open!
(preventing bugs and accidental objective crash)S
9.
“Image Processing” is for offline analysis of your images, please do not use it here
VIII.
Visual examination of the sample
1.
Put mechanical switch on the right hand side of the microscope to the ”Eye” position.
2.
Press “Locate” in the main microscope menu.
3.
Since this is an upright system, changing objectives can only be manually done by
rotating the objective revolver. Rotation should work very easily when objectives are in
the “up” position.
4.
For imaging or visual inspection, the objectives must be lowered entirely with the black
wheel in front of the microscope stand.
5.
Please make sure that the chosen objective corresponds to the one shown in the
software. If this is not the case, please restart the system. (Otherwise, your pinhole
and pixel sizes, as well as the sectioning thickness are all incorrect.)
6.
If you wish to view your sample in Brightfield mode, press the “TL” button
7.
For visual examination of fluorescence, press the buttons DAPI, GFP or RFP
8.
Before switching to acquisition mode, close transmitted and reflected light shutters by
pressing ‘off’ and turn off the Cool LED
by pressing ON/Off button
IX.
Acquiring confocal images
