1
Zeiss LSM800-02 + Airyscan confocal microscope
20/11/2019
System description:
I.
Upright confocal microscope ZEN blue 2.6 HotFix-8
II.
2 GaAsP +1 AiryScan detector (GaAsP)
III.
Motorized stage for multiple position imaging
IV.
Heating chamber
V.
NO CO
2
mixer
VI.
Multi-slide holder, dish holder, single slide holder
Objectives:
-
Position1: Objective Plan-Apochromat
10x / NA 0.45 Air
(WD=2.1mm)
-
Position2:
Objective Plan-Apochromat
20x / NA 1.0 W
DIC (WD=1.8mm)
-
Position3: Objective Plan-Apochromat
20x / NA 0.8 Air
-
Position4: Objective
Plan-Apochromat
40x/1.4 Oil DIC
(WD=0.13 mm)
-
Objective Plan-Apochromat
63x / NA 1.4 Oil
(not mounted)
-
Objective Plan-Apochromat 40x / NA 1.2 Water (not mounted)
Laser lines: 405, 488, 561, 640 nm
Detectors:
Two very sensitive Gallium arsenide phosphide photomultiplier tube (GaAsP PMTs) detectors
with free choice of the spectral range. (Uses a tunable short pass and band pass filter in front
of the PMTs)
Be very careful with using it to prevent instrument damage! If oversaturated, detectors will
switch off automatically.
1 GaASP
PMT for the
Airyscan
detector that can function as a normal detector in confocal
mode (detection range adjustable by a tunable short pass and a tunable long pass filter, no
bandpass filters)
An ESID detector for transmitted light.
Simultaneous detection of 3 dyes possible.
VII.
Starting up:
1.
Switch on the multiple socket (1) “
Hanging on the right side of the rack
”. Provides power
to the computer and microscope controllers
