Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
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5.2.4 Multidimensional Acquisition Settings
In the Multidimensional acquisition field (Fig. 82), the multidimensionality can be defined by checking
the corresponding boxes.
Fig. 82 Multidimensional input field – Z-Stack (with 10 slices), Time series (with 10 images) and
Regions active
The following dimensions are available:
Z-Stack – Acquisition of an image series in z
Time series – Acquisition of an image series in time
Bleaching – Acquisition of a time series including definition of a region to be bleached.
Tile Scan – Acquisition of an image series with neighboring field of views (FOV).
Positions
– Acquisition of an image series at different positions.
Regions – Imaging or manipulation of defined regions of interest (ROI)
The settings for multidimensional acquisition are accomplished within the tool groups
Acquisition
Parameter for Regions and Multidimensional Acquisition for all other functions. If available the
settings are displayed beside the function, e.g. how many images are taken for the time series (Fig. 82).
Corresponding tool bars within the tool groups only become available if the box of a multidimensional
function is checked; otherwise the tool bar is not displayed (Fig. 83 and Fig. 84). The
graphical display
field above the
Start Experiment
button shows a representation of the experiment type with its
dimensions is show. It also lists the estimated size of the final multidimensional image.
Fig. 83 Acquisition parameter Tool group